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We narrowed to 397 results for: abo.4

Showing: 181 - 200 of 397 results

  1. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...SSC-H / SSC-A gate would work just as fine (Figure 4). As H and A scale in proportion (while H and W do...                                                                                                         B.    Figure 4: (A) Using the proportional relationship between FSC-H...lymphocytes in a mouse tumor sample You made it! So much about the theoretical background. Let’s dive into the ...often choose a looser gate here. I am not worried about including irrelevant events in my analysis, as these...population, acquired from the previous gating pipeline, about 53% of my cells are dendritic cells and ~13% are...ve covered in this blog post. First, you learned about the types of scattering that occur when a cell is...

  2. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...fluorescent under acidic conditions, with pKa values below 4. Examples include mTurquoise2 (pKa = 3.1), tagRFP ... biology 27 (2015): 39-45. PubMed PMID: 26087123. 4. Kumagai, Akiko, et al. "A bilirubin-inducible fluorescent...deleting residues. Only about 10 amino acids can be deleted from the C-terminus and about 5 from the N-terminus...cylinder with a length of 4.2 nm and a diameter of about 2.4 nm (Hink et al., 2000). The complete beta-barrel...based on cofactor binding proteins as described above. The smallest tags are peptides that are engineered...

  3. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...mechanisms that cause plasmids to be incompatible. 4. RNA Extraction Without A Kit by Leah Schiesow We... highlighted reagent repositories and other collaborative efforts in the scientific community to speed...store my plasmid in TE, Tris, or water? And what about temperature? Find the details in this blog post....dominates DNA quantification discussions, but what about the other methods to quantify DNA? Here we cover...Incompatibility by Leah Schiesow If you're thinking about hosting multiple plasmids in one cell, it might ...

  4. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...to open scientific research data around the world. 4)      The Annals of Internal Medicine published systematic...study probably doesn’t say as much about open access as it does about the state of peer review. 8)     ...University Open Data Access (YODA) Project, “a collaborative endeavor guided by aspirations to promote open...

  5. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...targeting Golgi, ER, and endosome membranes) (Figure 4). The authors used SuperPLD to manipulate phosphatidic...fluorescent labeling via click chemistry.    Figure 4: SuperPLD hydrolyzes membrane PC to PA. A) OptoPLD... Check out our Optogenetics Guide to learn more about how these modules work, and read on to learn how...to the cell membrane isn’t powerful enough, what about altering the chemical composition of the membrane...

  6. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    ...topics from the Science by the Pint series. Chapter 4: The SITN Podcast, Sit'N Listen - In their podcast...To learn more about the STIN's podcast, email [email protected]    For more info about starting your...evens where scientists can grab a beer and chat about their work at a local bar. It doesn't have to be...1: Introduction - Vini and Amy tell us a little about themselves. Chapter 2: What is Science in the News...science communicators. In this chapter we learn about some of their events - science seminars, science...the lab and working with SITN. She points to collaboration and delegating as keys achieving success. Chapter...your own SciComm organization and to learn about some of SITN's other initiatives, email [email protected]...

  7. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ...: mouse, fruit fly, yeast, zebrafish, and worm. 4. Early Career Researcher Toolbox: Free Tools for Making...mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang Learn about mNeonGreen, mTFP1, and mWasabi and find plasmids...Broccoli, Spinach, and Corn, for example. Learn more about the aptamer soup in this blog post. 5. Five Popular...

  8. Molecular Biology Reference

    Type
    Guide
    ...which is transcribed from DNA. DNA is comprised of 4 nucleotides or bases, adenine, thymine, cytosine, ... into a double stranded helix. The order of these 4 nucleotides makes up the genetic code and provides...sequencing for plasmid verification DNA is made up of 4 bases, adenine, thymine , cytosine, and guanine. The...of interest. In vitro DNA replication requires the 4 nucleotides, a DNA polymerase enzyme, the template...addition to the normal A, T, C, or G nucleotide. The 4 standard bases are tagged with a different fluorophore...by DNA and RNA into proteins. DNA is comprised of 4 nucleotides Adenine (A), Thymine (T), Cytosine (C)...efficient and the machine cannot distinguish between the 4 labelled nucleotides. Epitope tags on the other hand...

  9. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    ...names of everyone at Addgene, and send random sets of 4 people out to lunch.   I think these random lunches...for the company, but also talking about sports, movies, and just about anything. As our organization grew... with our western blots. Thoughts about sharing Addgene is all about helping scientists share between ...culture of openness and trust that comes from caring about your colleagues on a personal level, and employees...often cite lunch as one of their favorite parts about working at Addgene. We’re a small non-profit, so...indication of the stiff competition or says something about my cooking.   This is a creative (and delicious)...for people in different labs to chat informally about their challenges, successes, and expertise. Who ...

  10. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...Addgene. 1 Lungu et al., Chem Biol. 2012 Apr 20;19(4):507-17. 10.1016/j.chembiol.2012.02.006. 2 Guntas...expression of cDNAs and shRNAs with a reporter gene, or (4) multiple sgRNAs with Cas9 for genome engineering....Sladitschek HL and Neveu PA. PLoS One. 2015. Apr 24;10(4):e0124958. LOC8-ing mitochondrial fusion with a...system was also designed for a short screening time (<4 hours) in order to avoid complicating effects from...improving the strength of the promoters used; and 4) optimizing expression of both the light sensor and...The proteins encoded in this set range in size from 4 to 156 kDa, enabling the user to characterise the ...new plasmid tool from the past year? Let us know about it in the comments or shoot us an e-mail at blog...

  11. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...facing towards or away from each other.  Figure 4: How to design a conditional allele with Easi-CRISPR...Biol. 18 (2017) (1):92. DOI: 10.1186/s13059-017-1220-4 PubMed PMID: 28511701 PubMed Central PMCID: PMC5434640... on CRISPR Mouse Models Learn about Homology Directed Repair Learn about Cas9 Ribonucleoprotein (RNP) ... a variety of species, including the standard laboratory mouse. It’s also made targeted insertions relatively...process of conventional gene targeting is lowered to about 2-3 months. But certain models remain more difficult... a mouse embryo to create chimeric mice. This laborious process takes ~8-10 months. With CRISPR (B), you...directly into a zygote and obtain a knockout mouse in about 3 months. Image courtesy of Wenning Qin. Single-stranded...

  12. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... Jan;103(1):303-313. doi: 10.1007/s00253-018-9485-4. Epub 2018 Nov 3. PMID: 30392122. Pfeiffer, Barret... system in Caenorhabditis elegans.” Nat Methods 9(4) (2012): 391-5. PubMed PMID: 22406855. PubMed Central...can be used in the repressible manner described above, as well as the inducible manner detailed in our...necessary to have all 3 of the different binary systems above if they all behave very similarly?” The simple answer... at the same time.  The 3 binary systems listed above are orthogonal, meaning that they do not affect ...Srikrishnan. “Introduction and expression of genes for metabolic engineering applications in Saccharomyces cerevisiae...

  13. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ...The Addgene-Seeding Labs plasmid grant is a collaborative endeavor providing plasmids to researchers in... of Zimbabwe, Zimbabwe Dr. Idah Sithole-Niang (above, left) is an Full Professor in Biochemistry with... CRISPR effect observed in bacteria. Learn more about the different CRISPR applications in plants.     ...Rodrigo Arturo Rivera Solís, Associate Professor (above, far right), thrives to make affordable biosensors...detect glyphosate via H2O2, one of the reaction’s metabolites. One useful genetically encoded H2O2 probe is... to the Addgene blog for updates and future collaborations. Many thanks to all of the awardees, all who...Additional Resources on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out...

  14. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...estimated that very little dystrophin correction (about 4%) is needed to see muscle improvement, with only...2015 Dec 31. pii: aad5143. PubMed PMID: 26721684. 4. Reardon S. Gene-editing summit supports some research...diseases we’d like to treat with CRISPR. As seen above, DMD can be treated with “one-size-fits-most” NHEJ-mediated.... Precision genome editing faces the challenges above and more. One chief challenge is upregulating HDR...

  15. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...are described in Nielsen et al (3) and Lou et al (4). Despite choosing proper register parts and designs...Science352.6281 (2016): aac7341. PubMed PMID: 27034378. 4. Lou, Chunbo, et al. "Ribozyme-based insulator parts...certain cellular behaviors, but little is known about the order or timing of these factors. Recombinase-based... three methods of state interrogation mentioned above (Sanger sequencing, qPCR, and fluorescent reporter-based...Bac Vectors Can Be Used for Genome Editing Learn about Michael Koeris' Journey from Grad Student to SynBio...

  16. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...ELISA, and our laboratory experience with peptide/protein analyses using ELISA. Peptides 72:4–15 . https:... theory, and trouble shooting. North Am J Med Sci 4:429 . https://doi.org/10.4103/1947-2714.100998 Additional... the thickness of the band provides information about the relative abundance of the protein in the sample...

  17. Antibody Guide

    Type
    Guide
    ...small multiplex experiments 4-color flow panel: BV421, FITC, PE, and APC 4-color microscopy panel: DAPI...protects mucosal membranes Dimer with a valency of 4 IgA1; IgA2 IgD Coexpressed with IgM on the surface... As a guideline, most antibodies can be stored at 4 °C for short-term storage. For long-term storage they...conjugated to fluorophores should never be stored below 4 °C. Certain isotype subclasses, such as IgG3, aggregate...aggregate after thawing and therefore should be kept at 4 °C. Record and track lot numbers for all antibodies...typically use HRP as a signaling molecule. Figure 4: A direct sandwich ELISA, which uses a capture antibody...are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments, each recognizing...

  18. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...that require a bachelor’s degree if you have a PhD. 4) Customize cover letters and even resumes The cover... your strengths in your resume and don't be shy about asking for help. The worst thing that can happen...

  19. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...order to protect their DNA from host cell nucleases (4). To further complicate matters, even when sequenced...PMID: 26761028. PubMed Central PMCID: PMC4728577. 4. Schmitz, Jonathan E., et al. "Rapid DNA library construction...our planet, but we still know collectively little about how they infect and influence the evolution of their...

  20. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    ...knocked out in the insulin signaling cascades in only 4 months compared to a year or greater with older technologies...be used in people until we learn more about safety and more about the how to use genome edited stem cells...because Jennifer did her graduate work one floor above mine in the lab of Dr. Jack Szostak, who went on...ceremony honored Charpentier and Doudna, who talked about their research leading up to the discovery of the...Charpentier met at a 2011 conference and decided to collaborate on what was a “curiosity” driven project. Both...importance of the strong, cross-disciplinary collaboration they shared to the work they did together. They...
Showing: 181 - 200 of 397 results