We narrowed to 835 results for: ang

...-884. PubMed PMID: 12718874. Yang, Junjie, Weihong Jiang, and Sheng Yang. "mazF as a counter-selectable...systems have been used as biological tools for a wide range of applications in molecular biology or in vivo ...production of MazE, the toxin becomes active. Shen Yang’s lab made use of mazF as a counterselectable marker...100% stability after five days. Jeff Hasty and Sangeeta N. Bhatia also used the hok/sok toxin-antitoxin... Jennifer Tsang...

...light scattered by that cell at a low angle (FSC) or large angle (SSC) (Figure 1). FSC values depend on...is scattered forward at a low angle (FSC) or to the side at a large angle (SSC). FSC and SSC depend on ...software in order to generate signals in a reasonable range. If colleagues in your group already have experience...ll notice that while the height of your events changes, the separation pattern of positive vs. negative...live population from my live-dead staining. (B) Changing the y-axis from SSC to FSC can alter the positioning...

... Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., & Zhang, F. (2013). Multiplex genome engineering...mutation. “Baby KJ” was diagnosed with a rare and dangerous metabolic disease shortly after his birth. Within...was able to tolerate bystander edits—unintended changes to nearby target bases. Up to 5 bystander adenine...However, all bystander edits produced synonymous changes, meaning the new codon corresponded to the same... editors to problematic mutations would need to change to target different mutations in different patients...j.cell.2015.12.041  Musunuru, K., Grandinette, S. A., Wang, X., Hudson, T. R., Briseno, K., Berry, A. M., Hacker...

...sensor relies on a change in localization to the cell membrane and not a direct change in the fluorescent...biosensor that has a binding affinity close to the range of concentrations expected in your samples. Higher...operating at the outer edge of the sensor’s dynamic range. With this in mind, Ed Boyden and colleagues set...family of fluorescent potassium indicators with a range of binding affinities (Torres Cabán et al. 2022)...sensors with lower binding affinities to broaden the range of applications. Image reproduced from Torres Cabán... B) LiLac responses are highly resistant to pH changes. Image adapted from Koveal et al. 2022, under CC-BY...

...10.1186/1471-2105-7-85Zhu G; Chang Y; Zuo J, Dong X, Zhang M, Hu G, Fang F. Fudenine, a C-terminal truncated...effects, cation concentrations and a temperature range) to predict PCR melting curves and dynamic melting... the board. If the samples or sample conditions change, the multiplexed protocol may have to be re-optimized..., which gives an RQ value representing the fold change of each sample compared to your control. But as...Wong, 2018.    🔥Hot Tip🔥 Negative fold changes in ΔΔCT are represented by RQs between 0 and 1.... during analysis can allow you to see this fold change as a positive difference (RQ>1), which may be easier...

...Cary Valley, Shreya Vedantam, Michelle Cronin, and Angela Abitua Thanks to a team effort from the Addgenies...Negative Selection for Plasmid Cloning by Jennifer Tsang There are many strategies for finding your clones...Proteins: mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang Learn about mNeonGreen, mTFP1, and mWasabi and ...Precision and Flexibility to CRISPR Editing by Jennifer Tsang Prime editing is a new CRISPR technique from David...insertions, deletions, and all possible base-to-base changes.   1. Plasmids 101: Transformation, Transduction... Jennifer Tsang...

...resolution. In this setup, gels have an increasing range of acrylamide content from top to bottom allowing...allowing a mix of proteins with a broad size range to be separated on a single gel.  Immobilizing proteins ...process is very effective in transferring a broad range of protein sizes but takes hours to overnight.  ...available dry blotting systems efficiently transfer a range of protein sizes in minutes without any transfer...being used and may require some trial and error (Yang et al., 2012).  Choosing your primary antibody Once...addition to protein abundance, westerns detect changes to a protein’s post-translational modifications...4350–4354 . https://doi.org/10.1073/pnas.76.9.4350 Yang P-C, Mahmood T (2012) Western blot: Technique, theory...

...undergoes a conformational change in response to the molecule it detects. This change then generates a reporter...sensing domain induces changes in a fluorescent signal based on ligand presence or changing cellular conditions... calcium induces CaM/RS20 interaction, thereby changing the FP’s structural organization and activating... or transcriptional activation, conformational changes, translation, and protein translocation. Targeting...

...positive outcome if the employee sees that real change is possible in your eyes so their confidence is...feedback is a very effective way to bring about change in behaviors and habits in a nonthreatening way... would you do differently? Beware of indirect language You must be honest about performance problems.... I see is managers who hint at or use indirect language to talk about real performance issues. Watch this...this little video to learn more about indirect language from Steven Pinker. As a manager you must be able...There is place in human discourse for indirect language, but it is not the way to go when giving feedback...

..., Wu X, Jiang W, Marraffini LA, Zhang F. Science. 2013 Jan; 339 (6121): 819–23. Mali P, Yang L, Esvelt...other CRISPR subtypes. Still, the large and dynamic range of CRISPR systems between and within prokaryotic...http://en.wikipedia.org/wiki/File:Crispr.png Barrangou R, Horvath P. Science. 315, 2007 Jan; 1709–1712...CRISPRdb. Date: 2013-09-18. Makarova K, Haft DH, Barrangou R, Brouns SJJ, Charpentier E, Horvath P, Moineu...

... The authors share a wealth of data on ... The language is ... The general representation of data is.....couple of sentences - even if written in a clumsy language make it much easier for me to go back and finish...about the paper after my initial read, adjust my language in the first (and clunky) version of my general...general comments and adjust them if my opinion changed after this more detailed look.        Encourage...try to stay away from the loaded and judgemental language. In each peer-review, I try to mention what I ... Tester, where she focuses on (1) salt-induced changes in root-to-shoot ratio in Arabidopsis, (2) expression...

...awarded to study 31 rare diseases,” said Claudia OuYang, BeHEARD Co-Director in a release. The research...developing metastatic melanomas and other cancers. Joshua Wang (bottom right), also of the Wistar Institute, says... broadly. “This [award] is pretty great for us,” Wang said. “We were planning on and looking at a bunch...number of stressors, including emotional stress or changes in temperature.  “The disease was first described...a rare disease that can cause hearing loss and changes in coloring of the hair, skin, and eyes. The researchers...of Waardenburg syndrome (WS4) by Pediatrician Duangrurdee Wattanasirichaigoon carried different mutations...

...they change color as they shift from "off" to "on" in the presence of calcium. This color change can be...imaging neurons, Griesbeck adds that this color change has a particular advantage for observing events...sensitivity. As reported in Nature Methods, the FRET changes of these Twitch sensor variants were optimized ... way to make a FRET sensor with a large dynamic range,” he says. Merkx recently applied this “trick” to...red-shifted FRET sensor for zinc using variants of mOrange2 and mCherry as donor and acceptor domains. He ...

...This post was contributed by guest blogger Eleanor Wang, a Research Specialist in Patrick Hsu's lab at UC...don’t tell us everything there is to know is a strange and exciting revelation. Science in any field can...were inspired by the imminent threat of climate change to study plant and marine biology, environmental...and while doing scientific research. It’s okay to change your mind about what exactly you want to do—I started...can think of to ask! You can DM me on Twitter @EleanorWang. I will do my best to answer or connect you with... help! Thank you to our guest blogger! Eleanor Wang is currently a Research Specialist in Patrick Hsu...other things too. You can follow her on Twitter @EleanorWang.     References and resources References Canning...

...membrane potential in response to visible light (Zhang et al., 2011). What do microbial rhodopsins and ...electrophysiological methods (Deisseroth, 2011; Mei and Zhang, 2012; Towne et al., 2016). Optogenetic neuromodulation...neural activity (Bernstein and Boyden, 2011; Mei and Zhang, 2012). For example, blue light activates channelrhodopsins...Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit mapping." Journal of Neuroscience 28.28 ...237-243. PubMed PMID: 20882. Mei, Yuan, and Feng Zhang. "Molecular tools and approaches for optogenetics.... PubMed PMID: 12665802. SSharma, Suveena, and Jinfang Zhu. "Immunologic applications of conditional gene...PMID: 28772120. PubMed Central PMCID: PMC5830081. Zhang, Feng, et al. "The microbial opsin family of optogenetic...

...followed suit. Two Addgenies, Susanna Bachle and Yijun Zhang, recently attended virtual conferences (TAGC and...and ASGCT, respectively) and Addgenie Angela Abitua hosted a virtual Reproducibility for Everyone workshop...host will see the highly voted questions,” says Zhang. “Usually the moderator starts by asking the speaker... hosted in breakout rooms, or even on social media.Zhang was able to view posters without presenters “...to start. “There was not much time to network,” Zhang says. “The schedule was pretty busy, but after the... Jennifer Tsang...

...bioreporter spontaneously changes from green to orange such that non-growing cells are orange, slow growing cells... But the shape and metabolism of leaves can be changed by light and temperature and leaf autofluorescence...cells are orange-green, and fast growing cells are green. A second colonizer, labelled with a constitutively... Jennifer Tsang...

...meetup.com! Many thanks to our guest blogger Angela Kaczmarczyk! Angela Kaczmarczyk is a co-organizer at BosLab...2015, there were six of us. We were initially strangers but we all shared a passion for DIY Biology and...biohacking courses. Our members pursue a diverse range of projects at BosLab. Some are entrepreneurs using...RNA with small molecules. Other members’ projects range from hacking probiotics to filming educational lab... Angela Abitua...

...B978-012198382-6/50026-1 Wang R, Xiang S, Feng Y, Srinivas S, Zhang Y, Lin M, Wang S (2013) Engineering production...for specificity and sensitivity, for example by changing certain amino acids to improve its stability. ...single protein that can recognize the target protein (Wang et al., 2013). These proteins are also plasmid-derived.../10.1016/j.bios.2016.04.091 Cruse JM, Lewis RE, Wang H, Geziena MT, Steven GE, Lorna J. (2004) ANTIGENS...

... a broad pH range. The four biometric probes successfully targeted endosomes under a range of pH conditions...and RNA expression - but what if you just want to change protein levels? Until recently, no such tool existed...proliferation. These cells also did not contain changes in expression of TRIM21-associated genes, indicating...transcription factors in series and independently changes inducer levels. That is, if transcription factor...expression of a reporter protein, it is possible to change the amount of noise in reporter gene expression...