We narrowed to 315 results for: bon;
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TypeBlog Post...only occur when galactose replaces glucose as the carbon source. Student results have shown that most, but...
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Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog Post...(about 2.7kb total)—is inserted into a plasmid backbone for delivery to the cells. As a precautionary ... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...Encodes one insert, encodes gRNA/shRNA, or empty backbone. Purpose Answer “What does this plasmid ... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post...eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins (RNPs) failed to induce off-target editing... -
Plasmids 101: FLEx Vectors
TypeBlog Post...Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck NB. A directional strategy for... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog Post...before the event... Choose a moderator with a backbone. It is nice to have a subject area expert, but... -
Plant Plasmids and Resources
TypeCollection...top Empty Backbones The table below highlights plasmids that can be used as empty backbones for your gene...CRISPR Cloning Transcription Factors RNAi Empty Backbones Fluorescent Proteins Other Resources Background...different functional variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR resources...expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice. Includes a protocol (Link opens...includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ... -
Polymerase Chain Reaction Overview and Applications
TypeBlog Post...the dsDNA template to a point where the hydrogen bonds break between the base pairs. This results in the... -
Plasmids 101: Protein Expression
TypeBlog Post... include ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free expression systems... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post...diluted from 10X and containing both Hepes and bicarbonate) is brought to room temperature and placed in... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post...Protein Timers Browse All Fluorescent Protein Empty Backbones Check Out Our Fluorescent Protein Guide ... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post...what do you want to make? References: Jerome Bonnet et al. "Amplifying genetic logic gates." Science... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...is irreversible — FP fragments become covalently bonded by associating with one another — which means it... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post.... S. cerevisiae) Plasmid Type: Choose an empty backbone if you intend to clone your own insert, or use... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post...enCh IP technology using recombinant CRISPR ribonucleoproteins." Genes to Cells 21.4 (2016): 370-377. PubMed... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog Post...periplasmic space to be oxidized and form disulfide bonds (Frenzel et. al., 2013). If you’re using a mammalian... -
DNA Purification Without a Kit
TypeBlog Post...extraction protocol) disrupts the DNA’s hydrogen bonds with water molecules and coaxes it into sticking... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...immediate co-transformation with linearized vector backbone. DpnI digestion - 1 μl DpnI is added to the... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post...Maleimide-labeling occurs when a maleimide group forms a covalent bond with a sulfhydryl group (–SH), such as that found... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post...eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins (RNPs) failed to induce off-target editing...