We narrowed to 324 results for: cloning

If you’re using CRISPR to make a genome edit, how do you know if your experiment was successful? Try using fluorescent CRISPR reporters like SRIRACCHA and GEmCherry2.

Interview with Professors Eric Mendenhall and Richard Myers about their CRISPR- based system for tagging any endogenous gene of interest.

Find information on Addgene's collection of plasmid-based antibodies, including monoclonal antibodies, scFvs, and nanobodies.

Learn about the many fluorescent protein tools enabled by the nanoLuc luciferase.

List of plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE plasmids.

Find plasmids and resources for FRET experiments to measure protein interactions or conformation changes.

Find materials created at Addgene, including empty backbones, control vectors, viral packaging and expression plasmids, and more.

Twenty years have gone by fast! Join us to share some highlights of Addgene and our community as we close out our 20th anniversary celebration.

Explore Addgene's collection of luciferase plasmids for gene expression assays and bioluminescent reporters.

Learn how you can use Green Listed to design gRNAs for custom CRISPR screens.

Learn about the pros and cons of biased and unbiased CRISPR genotyping methods.

Learn about Addgene's QC process and how and why we maintain a high standard of sequence accuracy across our plasmid collection.

Read how the Wu lab is screening over 3,000 antibodies in mice and human brain IHC to verify antibodies for addition to the mAb3D atlas website.

Learn how to use the dTAG system to inducibly degrade your protein of interest.

This post provides an overview on how transcription terminates in prokaryotes and eukaryotes.

Find information on CRISPR mammalian expression systems and delivery methods. Use the table to quickly compare between the methods.

Browse a diverse set of wild-type malate dehydrogenase genes and watermelon mutant clones for your course-based undergraduate research experience (CURE) projects.

an overview of qPCR and ddPCR copy number variant assays.

Mobile-CRISPRi is a set of modular CRISPRi tools that can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration and is easily transferred via conjugation.

Learn some best practices to keep your mammalian cell lines free of contaminants and your experiments running smoothly.