We narrowed to 324 results for: cloning

...Video Restriction Cloning Assemble plasmids by restriction digest and ligation Cloning by PCR Generate ...single reaction Ligation Independent Cloning (LIC) Scarless cloning with Type II restriction enzymes and...and T4 polymerase pLKO.1 - TRC Cloning Vector Cloning protocols for using the pLKO.1 vector, a backbone...can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations... the Lab Bench Basic Molecular Biology Plasmid Cloning Virus Antibodies Intro to the Lab Bench Introductory...tissues or cells without a commercial kit Plasmid Cloning Protocols for constructing and analyzing your plasmids...restriction sites by PCR Modification by Annealed Oligo Cloning Add a short stretch of DNA to a plasmid Gibson ...

...PCR - or restriction enzyme -based cloning to test individual clones before use of more expensive forms...digested with restriction enzymes common to multiple cloning sites, will result in similar sized bands, thus...Insert Orientation by Restriction Digest If you clone an insert into a vector by a single restriction ... enzyme, you will need to verify that it has be cloned in the correct orientation - this can be done by...restriction digest. Although this is never an ideal cloning strategy, sometimes it cannot be avoided. If you...therefore the only thing left to do is identify the clone(s) in which the insert is in the correct orientation... want to know how to differentiate between two clones that differ only in the orientation of the insert...

...Changing a Multiple Cloning Site (MCS) Adding short tags - ex: HA or Flag Cloning shRNAs Background For...using oligo overlap cloning. Protocols... Protocols Plasmid Modification by Annealed Oligo Cloning Plasmid Modification...Modification by Annealed Oligo Cloning You may also like... Restriction Digest of Plasmid DNA DNA Ligation Bacterial...Bacterial Transformation Summary Oligo overlap cloning can be used anytime you need to add a short stretch...NdeI, PacI, AscI, and MfeI. With oligo overlap cloning, you can design a set of oligos containing your...design overlapping oligos that once annealed can be cloned directly into the overhangs generated by restriction...

...target species, target name, host organism, isotype, clone details, vendor, method, tissue preservation, target...

...Formosa has deposited six plasmids with a multiple cloning site in place of the epitope tag for creation of...

...pLKO.1 TRC-Cloning Vector I.2 Recipes I.3 Warranty information Back to Top A. pLKO.1-TRC Cloning Vector A...these bases are important for cloning the oligos into the pLKO.1 TRC-cloning vector. Forward oligo: 5’ CCGG...GTATAGCTTAACGTAGGCATT 3’ Back to Top C. Cloning Oligos into pLKO.1 The pLKO.1-TRC cloning vector contains a 1.9kb stuffer...digests. Digest pLKO.1 TRC-cloning vector with AgeI. Mix: 6 μg pLKO.1 TRC-cloning vector (maxiprep or miniprep...Sequence of pLKO.1 TRC-Cloning Vector Click here to see the sequence of pLKO.1 TRC-cloning vector. The vector...Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting... Protocols pLKO.1 Protocol pLKO.1 - TRC Cloning Vector Addgene Plasmid 10878. Protocol Version ...

...Plasmids Browse our selection of empty vectors for cloning in shRNAs. Most plasmids are in a lentiviral backbone... Additional Resources Addgene Resources pLKO.1 cloning vector protocol Other Addgene viral protocols Lentiviral...

...easy creation of long hpRNA with Gateway-based cloning and straightforward positive selection of plants...assembly of intron-spliced hpRNAs through Golden Gate cloning. Artificial micro RNA and short hairpin RNA The...

...Options”, including adding adapter sequences for cloning purposes.  Press run and wait for the generation...this purpose.  The gRNA oligos are subsequently cloned into your vector of choice using the adapter sequences...

...plasmids The transfer plasmid is the vector you clone your gene of interest into and is an important feature...Lentiviral production and transduction Once you have cloned your gene of interest into lentiviral transfer ...

...should be avoided whenever possible. Other popular cloning strategies for the assembly of synthetic parts ...systems such as MoClo . Check out our Molecular Cloning Techniques Guide for an overview of the most common...

... passing. We’ve recently piloted Addgene’s new cloning grade DNA service, and viral vector distribution...

...ITRs does not get packaged in the AAV vector. The cloning capacity between the ITRs is ~4.4 kb. ITRs are ...

...resources on the Addgene blog Learn about Gateway cloning Read about genome engineering in flies Learn more...

...assembled with other elements using golden gate cloning. This toolkit is designed to be used across many...

...thanks to their small size of 12–15 kDa Ease of cloning and expression from plasmids, due to only needing...

...Pathway Clone Collection 2.0: Set of vectors from the Esposito Lab for use with the Gateway® Cloning Platform...Pathway Ras Pathway You May Also Like: Ras Pathway Clone Collection 2.0 mTOR Pathway p53 Pathway Background...

... Protocols Subcloning Plasmid Cloning by Restriction Enzyme Digest (aka Subcloning) You may also like...Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental...your recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid...test digest later to verify that the insert was cloned in the correct orientation. If you cannot find ...sites to flank your insert : You can use PCR Based Cloning and add restriction sites to the ends of your oligos...of your recipient plasmid using Annealed-oligo Cloning . If you are lucky enough to have multiple options...instructions for your competent cells. For most standard cloning, you can transform 1-2μl of your ligation reaction...

...commonly used in molecular cloning techniques, such as PCR or restriction cloning . It is also used to quickly...typically involves ∼500 ng of DNA, while molecular cloning often requires 1 µg of DNA. The total reaction ...sufficient. For digests with >1 µg of DNA used for cloning, it is recommended that you digest for at least...finding an enzyme that cuts your vector's multiple cloning site (MCS), but does not cut your insert, you could...prevent re-circularization of the vector if you are cloning in an insert. CIP (calf alkaline phosphatase) or...

...proteins and empty backbones for expression and cloning of fusion proteins, along with key parameters to...506 517 93 5.7 10 min Monomer pCS2+mNeonGreen-C Cloning Vector - Mammalian Expression Return to top Yellow...Zebrafish/Xenopus/Worm/Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression mCitrine-pBAD...mOrange 548 562 49 6.5 2.5 h Monomer pET mOrange LIC cloning vector (u-mOrange) - Bacterial Expression pCS2+...pmScarlet_C1 - Mammalian Expression pCS2+mScarlet-C Cloning Vector - Mammalian Expression mScarlet-I 569 593...587 610 16 4.5 15 min Monomer pcDNA3 mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry ...Zebrafish/Xenopus/Worm/Sea urchin pET mCherry LIC cloning vector (u-mCherry) - Bacterial Expression pFA6a-link-yomCherry-SpHis5...