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We narrowed to 332 results for: cloning

Showing: 181 - 200 of 332 results

  5. Fluorescent Proteins: FRET

    Type
    Collection
    ...their properties. Where available, we have listed cloning backbones and control plasmids. Donor Acceptor ....8 6.2 3.1 pmTurquoise2-N1 , pCS2+mNeonGreen-C Cloning Vector , mNeonGreen-mTurquoise2 CyPet YPet 435 ....80 592 128,000 0.45 6.4 4.7 pCS2+mNeonGreen-C Cloning Vector , pcDNA3-mRuby2 , mNeonGreen-mRuby2-FRET...mVenus, and Amber (mVenus Y67C) FRET standards and cloning backbones Koushik et al. (2006) Cerulean, Venus...two-hybrid FRET Positive and negative controls and cloning backbones for mTurquoise2-mVenus(L68V) Goedhart...yield of 93% Positive and negative controls and cloning backbones for various FRET pairs with mTurquoise2...mTurquoise2 Positive and negative controls and cloning backbones for red, yellow, and turquoise FRET pairs...

  6. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ... Protocols Subcloning Plasmid Cloning by Restriction Enzyme Digest (aka Subcloning) You may also like...Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental...your recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid...test digest later to verify that the insert was cloned in the correct orientation. If you cannot find ...sites to flank your insert : You can use PCR Based Cloning and add restriction sites to the ends of your oligos...of your recipient plasmid using Annealed-oligo Cloning . If you are lucky enough to have multiple options...instructions for your competent cells. For most standard cloning, you can transform 1-2μl of your ligation reaction...

  8. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ...commonly used in molecular cloning techniques, such as PCR or restriction cloning . It is also used to quickly...typically involves ∼500 ng of DNA, while molecular cloning often requires 1 µg of DNA. The total reaction ...sufficient. For digests with >1 µg of DNA used for cloning, it is recommended that you digest for at least...finding an enzyme that cuts your vector's multiple cloning site (MCS), but does not cut your insert, you could...prevent re-circularization of the vector if you are cloning in an insert. CIP (calf alkaline phosphatase) or...

  9. Luciferase Plasmid Collection

    Type
    Collection
    ...popular empty backbone plasmids into which you can clone your regulatory element or gene of interest into... of 5' promoter/enhancer regions using Gateway cloning. Jorge Ferrer 16539 pBV-Luc Firefly Insertion of...Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2-ccdB-Nanoluc NanoLuc...Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival. Mikko Taipale 87078 pLenti6.2-Nanoluc-ccdB...Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X Firefly...Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 106253 pLS-mP-Luc Firefly Insertion... can be used for screening of transformants in cloning process. Lynne Postovit 64034 pGL4Luc-RLuc Firefly...

  10. Viral Vectors 101: Transductions

    Type
    Blog Post
    ... Then, your desired DNA to be integrated can be cloned into the vector that you have selected. The nucleic...

  19. CRISPR Plasmids - Tagging

    Type
    Collection
    ...currently unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target... target sequence, as well as design and clone in the homology arms for the donor plasmid. The Mendenhall... also provided their protocol for homology arm cloning: Mendenhall & Myers FETCh-seq Protocol 134.7 KB... complexes. This vector serves as a backbone to clone the left and right homology arms. More details on...al. Cell Rep. 2015 . Alternatively, cDNAs can be cloned directly into this vector and targeted to the AAVS1...include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt Unc-32::GFP targeting vector...

  20. Gibson Assembly Protocol

    Type
    Protocol
    ... Protocols Gibson Cloning Gibson Assembly Cloning You may also like... Cloning Methods...Assembly Cloning! Protocols...biologists now use this method extensively. Why Gibson Cloning? No need for specific restriction sites. Join almost...
Showing: 181 - 200 of 332 results