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We narrowed to 326 results for: cloning

Showing: 181 - 200 of 326 results

  1. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...plasmids The transfer plasmid is the vector you clone your gene of interest into and is an important feature...Lentiviral production and transduction Once you have cloned your gene of interest into lentiviral transfer ...

  2. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...pLKO.1 TRC-Cloning Vector I.2 Recipes I.3 Warranty information Back to Top A. pLKO.1-TRC Cloning Vector A...these bases are important for cloning the oligos into the pLKO.1 TRC-cloning vector. Forward oligo: 5’ CCGG...GTATAGCTTAACGTAGGCATT 3’ Back to Top C. Cloning Oligos into pLKO.1 The pLKO.1-TRC cloning vector contains a 1.9kb stuffer...digests. Digest pLKO.1 TRC-cloning vector with AgeI. Mix: 6 μg pLKO.1 TRC-cloning vector (maxiprep or miniprep...Sequence of pLKO.1 TRC-Cloning Vector Click here to see the sequence of pLKO.1 TRC-cloning vector. The vector...Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting... Protocols pLKO.1 Protocol pLKO.1 - TRC Cloning Vector Addgene Plasmid 10878. Protocol Version ...

  3. CRISPR Plasmids - Tagging

    Type
    Collection
    ...currently unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target... target sequence, as well as design and clone in the homology arms for the donor plasmid. The Mendenhall... also provided their protocol for homology arm cloning: Mendenhall & Myers FETCh-seq Protocol 134.7 KB... complexes. This vector serves as a backbone to clone the left and right homology arms. More details on...al. Cell Rep. 2015 . Alternatively, cDNAs can be cloned directly into this vector and targeted to the AAVS1...include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt Unc-32::GFP targeting vector...

  9. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...promoter. WPRE has been removed to increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express...well as the Cre and Flp recombinases. Genes can be cloned into these transfer vectors using shuttle vectors...pAdx-CMV-LacZ is uniquely suited to simplify the cloning process as it can be used in blue-white screening...Xgal/IPTG plates — if your insert is successfully cloned into this vector, transformants will instead form...

  10. Lentivirus Plasmids

    Type
    Collection
    ....1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid; includes a stuffer for easy cloning Root 14748...shRNA. See article for detailed information about cloning & additional plasmids. Aebischer and Trono 11795...cassette (promoter and gene of interest) can be cloned into the plasmid. Nolan 12257 pWPXL 2nd EF-1alpha... the gene of interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in...

  11. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ... Protocols Subcloning Plasmid Cloning by Restriction Enzyme Digest (aka Subcloning) You may also like...Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental...your recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid...test digest later to verify that the insert was cloned in the correct orientation. If you cannot find ...sites to flank your insert : You can use PCR Based Cloning and add restriction sites to the ends of your oligos...of your recipient plasmid using Annealed-oligo Cloning . If you are lucky enough to have multiple options...instructions for your competent cells. For most standard cloning, you can transform 1-2μl of your ligation reaction...

  12. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ...commonly used in molecular cloning techniques, such as PCR or restriction cloning . It is also used to quickly...typically involves ∼500 ng of DNA, while molecular cloning often requires 1 µg of DNA. The total reaction ...sufficient. For digests with >1 µg of DNA used for cloning, it is recommended that you digest for at least...finding an enzyme that cuts your vector's multiple cloning site (MCS), but does not cut your insert, you could...prevent re-circularization of the vector if you are cloning in an insert. CIP (calf alkaline phosphatase) or...

  16. Viral Vectors 101: Transductions

    Type
    Blog Post
    ... Then, your desired DNA to be integrated can be cloned into the vector that you have selected. The nucleic...
Showing: 181 - 200 of 326 results