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We narrowed to 466 results for: organization Addgene

Showing: 181 - 200 of 466 results

  1. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...mutagenizing a population (typically cells or small organisms) and then assaying for a functional or phenotypical... events. To put it another way, only cells or organisms with the desired phenotype will survive and be.../10.1002/iub.165.  Additional resources on the Addgene Blog CRISPR libraries Genome-wide screens with...

  2. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...antiparallel configuration), typical structures known as Organized Smooth Endoplasmic Reticulum (OSER) whorls are...Besides the data, new plasmids and/or cells or organisms that are engineered can be shared to simplify ...based biosensors.     Additional Resources on the Addgene Blog Which Fluorescent Protein Should I Use? Choosing... Protein Biosensors Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find...

  3. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...Additional Resources on the Addgene Blog Read Our Tips to Keep Your Lab Notebook Organized Learn about Software... making similar mistakes in the future. Stay organized Set up a table of contents in the top directory...Software Matchmaker Additional Resources on Addgene.org Find Protocols for Your Next Experiment Find...

  4. Lab Automation at Addgene

    Type
    Blog Post
    ...to laws concerning genetically modified organisms. Since Addgene is very proud to help scientists share...times easier. We’ve certainly felt this way at Addgene so we’ve been putting some work into finding robots... many more robots than you ever knew existed! Addgene recently started automating a few protocols to ... hour. Automating vial fiIling The majority of Addgene plasmids are shipped as agar stabs in glass vials... easily programmed for a variety of lab needs. Addgene uses it to fill as many vials as it can in the ...labels than your hand and help keep things more organized. Cap tubes with a micronic cap sealer. This is...avoid sore fingers. There are other process that Addgene would love to move forward and automate. For instance...

  5. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...information to Addgene Material Transfer Agreement (MTA) approval  Send plasmids to Addgene  Quality control... field, and contact Addgene. We may require that you send your samples to Addgene pre-transformed in this... to appear in your Addgene account under My Plasmids, we need to know your Addgene account username. Shipping...  Additional Resources on the Addgene Blog Find Articles Citing Addgene Plasmids Searchable and Sortable...the spreadsheet to deposit@addgene.org with the following information: Addgene account username. In order...Suggest A Plasmid You'd Like to Find at Addgene Resources on Addgene.org Start Your Deposit Deposit FAQ Which...tech transfer team will communicate with your organization’s technology transfer office or other authorized...

  6. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    ...feature interviews with real Addgenies who have a variety of roles at Addgene.  You’ll hear all about their.... Along with our role as a plasmid repository, Addgene aims to be a vital educational resource for scientists...mind, we are very excited to debut our brand new Addgene Videos page! Videos are a powerful tool for communicating...scientific knowledge. On this new page, we have neatly organized the educational video content from our website...or to answer any questions you might have about Addgene. The page is divided into three main sections: ...feature screen-capture footage to help you navigate Addgene’s vast online resources. Our most popular How-To...stage of the selection and checkout process on Addgene’s website: We’ll be continuously updating this...

  7. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ...materials. Addgene will then apply for the license for distribution to the requester’s organization. A non-refundable...Addgene is excited to announce we now offer an export license application for rabies materials! Some ... export-controlled materials in the USA, where Addgene is located.  The export license, if granted, allows... export control license, once approved, allows Addgene to ship plasmids or viral vectors containing sequences...processing costs. Once the application is approved, Addgene then ships the material following our normal process... If you have any questions about this or other Addgene shipping and ordering processes, don’t hesitate...contact our friendly customer support team at help@addgene.org.  Curious about how rabies plasmids are used...

  8. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...integrins (cell-adhesion proteins) targeting different organs. This means that exosomes not only promote microenvironment...microenvironment’s malignancy but also incite different organs to become malignant. Exosomes also contain miRNA...PubMed PMID: 20036813. Additional resources on the Addgene blog Read our cancer related blog posts Learn ...  Find plasmids for your research Learn about Addgene's viral service ..., et al. "Tumour exosome integrins determine organotropic metastasis." Nature 527.7578 (2015): 329. PubMed...about the tumor microenvironment Resources on Addgene.org Visit our cancer research plasmids and resources...

  9. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ... field. Resources at Addgene Browse Addgene's RNAi resources Browse Addgene's CRISPR resources Read ...countries in number of plasmid requests from Addgene. Addgene - Canton Nucleic Acids Forum 2015 from Joanne...was pleased to be able to participate when the organizers of the Canton Nucleic Acids Forum (CNAF) 2015...conference invited me to attend and speak about Addgene’s experience helping scientists share plasmids, ...variety (see slides from my talk below). Largely organized by Dmitry Samarsky, and a very welcoming team ... talk to so many scientists who were potential Addgene requestors and depositors. China is in one of the...conference was kicked off by Nobel Laureate (and Addgene depositor) Craig Mello. Taking his groundbreaking...

  10. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ....2024.100519 More resources from Addgene Plasmids 101: Modular Cloning Addgene's CRISPR Guide Plasmids 101: ...been looking for just the right CRISPR vectors on Addgene and found instead ones that were… pretty close,...Fragmid components are available for request on the Addgene website, both as individual plasmids and as the...component available on the Fragmid website, or through Addgene, has worked well in the hands of the Doench lab...available. Table 1: Fragmid destination vectors Organism Delivery Type Destination Vector Mammalian...select the features of your vector, which are: Organism (Mammalian or drosophila) Delivery type (lentivirus...the ID to look up the component plasmids on the Addgene website. Step 4: Naming your assembled plasmid...

  11. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources on the Addgene blog Learn...about Addgene's Lab Tips video series Resources on Addgene.org Find lab protocols on the Addgene website...Since rolling out our viral service in 2016, Addgene has shipped thousands of AAV and lentiviral preps... purity of all AAV viral lots. Aligning with Addgene’s mission to “accelerate research and discovery ...improve your experiment results! Subscribe to the Addgene YouTube page! AAV purification by iodixanol gradient...cellular level instead of having to work with a whole organism and can help scientists produce vital tools in...website Watch Addgene's video collection ...

  12. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ...developmental biologists have to extract RNA from their organism of interest, use the RNA to create a complementary... in  the labeling specific cell types or entire organs in planarians. For example, the gene Piwi labels... which is similar to PR-T4P, is available from Addgene. If you use these published protocols and want ...genes, we encourage you to deposit them back with Addgene and expand the number of genes that other classrooms... PMCID: PMC304099. Additional Resources on the Addgene Blog Arabidopsis in Education Learning Synthetic...Girardia sp., Dugesia dorotocephala, Phagocata morgani, and Phagocata gracillis. There are 11 different...strain that is designated as DE3. For Phagocata morgani and Phagocata gracillis, the process is a little...

  13. RNA Extraction Without A Kit

    Type
    Blog Post
    ...proteins are retained in the interphase and bottom, organic layer. The top aqueous layer is extracted and RNA...layer to avoid carryover of DNA from the bottom, organic layer. Another trick is to precipitate the RNA ...10.1038/nprot.2006.83 Additional resources on the Addgene blog Read blog posts related to plasmid protocols...for purifying DNA without a kit Resources on Addgene.org Check out our molecular biology protocols Find...

  14. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...More…” menu. You can also follow companies and organizations to see their news and job postings. 4) Use ...connections can connect you to people at that organization. This type of connection can help you gather...network and it will reward you. Resources on the Addgene Blog Find tips for online social networking for...that is not relevant to business or volunteer organizational work. LinkedIn is not the platform for politics...

  15. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ... a signaling pathway in the context of a whole organism, but also allows for precise activation of a kinases..., but they need to be expressed in a tissue or organism that blue and violet light can penetrate. How ...PMCID: PMC4097317. Additional Resources on the Addgene Blog Learn More about Photoactivatable Fluorescent...Control Protein Activity with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable...

  16. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...While C. elegans are often thought of as simple organisms , they have many levels of complexity, especially...Makerspaces like ours can also make accounts with  Addgene. We now have the incredible CRISPR/cas9 tools at...collection of samples and sequencing is being organized and funded by someone else (KittyBiome has been...

  17. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    ...Foundation's Linda Kahl explains the Addgene collaboration this way: "Addgene is one of the leaders in distributing...to have Addgene as a professional public-benefit partner who shares our core values." Addgene spoke with...bioeconomy. Addgene: What do these BIOFAB and BIL gates kits – the first to be made available via Addgene and ...a co-founder of the BioBricks Foundation, an organization working to advance biological engineering openly...gates built from transcriptors are available at Addgene as a BIOFAB kit comprised of well-characterized... promises and associated sequence information. Addgene has partnered with the BioBricks Foundation to ...We have found an amazing and valued partner in Addgene," Endy added. "The phrase we like to use is 'the...

  18. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...terms in an Addgene plasmid search and found the results confusing, I’m sure you’ll find Addgene’s newly launched...Additional resources on the Addgene blog Learn more about other features on Addgene's website ... better way to search First, check out the new Addgene homepage. You may have noticed the search field...indicate that their plasmids are available from Addgene in the Methods & Materials or elsewhere in their...from the James Trimmer Lab published in eLife. Addgene is currently distributing 94 plasmids expressing...someone has already created and deposited it with Addgene. Combining search terms with filters lets you drill...to apply a wide range of filters isn’t new to Addgene’s plasmid search. The new design of the plasmid ...

  19. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...want to pick a topic and have no one show up. At Addgene, we wanted to think more closely about the wealth...: Discussing next-generation sequencing at the Addgene bioinformatics journal club! What happens during...figure. Some follow the same figure by figure organization with a different person responsible for each...messaging but we as scientists often prefer data. At Addgene we rely on polls and surveys. A mix of closed (...most likely the primary drivers of frequency. At Addgene, we opted to start with one per month. When to ...journal clubs should be well planned and carefully organized. Most importantly, they should be regularly assessed...own journal club! Additional resources on the Addgene blog Read our blog posts centered around scientific...

  20. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... & Small Molecule Fluorophores. Addgene Blog. https://blog.addgene.org/better-dyeing-through-chemistry-small-molecule-fluorophores... is now the most popular self-labeling tag in Addgene’s collection. CLIP-tag: Also in 2008, Kai Johnsson...Various Many Complicated   Applications Organic dyes are typically both brighter and more photostable...at a price, though. Purchasing lots and lots of organic dyes or specialized ligands is more expensive than...are the most popular self-labeling enzymes in Addgene’s collection, so you may be able to get a plasmid...ligands brings up bad memories of undergraduate organic chemistry lab, don’t worry – many SNAP-tag and ...ligand to use for your experiment, and explore Addgene's collection to find self-labeling fusion proteins...
Showing: 181 - 200 of 466 results