...developmental biologists have to extract RNA from their organism of interest, use the RNA to create a complementary... in  the labeling specific cell types or entire organs in planarians. For example, the gene Piwi labels... which is similar to PR-T4P, is available from Addgene. If you use these published protocols and want ...genes, we encourage you to deposit them back with Addgene and expand the number of genes that other classrooms... PMCID: PMC304099. Additional Resources on the Addgene Blog Arabidopsis in Education Learning Synthetic...Girardia sp., Dugesia dorotocephala, Phagocata morgani, and Phagocata gracillis. There are 11 different...strain that is designated as DE3. For Phagocata morgani and Phagocata gracillis, the process is a little...

...Foundation's Linda Kahl explains the Addgene collaboration this way: "Addgene is one of the leaders in distributing...to have Addgene as a professional public-benefit partner who shares our core values." Addgene spoke with...bioeconomy. Addgene: What do these BIOFAB and BIL gates kits – the first to be made available via Addgene and ...a co-founder of the BioBricks Foundation, an organization working to advance biological engineering openly... built from transcriptors are now available at Addgene through the Biobrick Public Agreement (BPA) Plasmid... promises and associated sequence information. Addgene has partnered with the BioBricks Foundation to ...We have found an amazing and valued partner in Addgene," Endy added. "The phrase we like to use is 'the...

...More…” menu. You can also follow companies and organizations to see their news and job postings. 4) Use ...connections can connect you to people at that organization. This type of connection can help you gather...network and it will reward you. Resources on the Addgene Blog Find tips for online social networking for...that is not relevant to business or volunteer organizational work. LinkedIn is not the platform for politics...

...proteins are retained in the interphase and bottom, organic layer. The top aqueous layer is extracted and RNA...layer to avoid carryover of DNA from the bottom, organic layer. Another trick is to precipitate the RNA ...10.1038/nprot.2006.83 Additional resources on the Addgene blog Read blog posts related to plasmid protocols...for purifying DNA without a kit Resources on Addgene.org Check out our molecular biology protocols Find...

... a signaling pathway in the context of a whole organism, but also allows for precise activation of a kinases..., but they need to be expressed in a tissue or organism that blue and violet light can penetrate. How ...PMCID: PMC4097317. Additional Resources on the Addgene Blog Learn More about Photoactivatable Fluorescent...Control Protein Activity with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable...

...While C. elegans are often thought of as simple organisms , they have many levels of complexity, especially...Makerspaces like ours can also make accounts with  Addgene. We now have the incredible CRISPR/cas9 tools at...collection of samples and sequencing is being organized and funded by someone else (KittyBiome has been...

...terms in an Addgene plasmid search and found the results confusing, I’m sure you’ll find Addgene’s newly launched...Additional resources on the Addgene blog Learn more about other features on Addgene's website ... better way to search First, check out the new Addgene homepage. You may have noticed the search field...indicate that their plasmids are available from Addgene in the Methods & Materials or elsewhere in their...from the James Trimmer Lab published in eLife. Addgene is currently distributing 94 plasmids expressing...someone has already created and deposited it with Addgene. Combining search terms with filters lets you drill...to apply a wide range of filters isn’t new to Addgene’s plasmid search. The new design of the plasmid ...

...want to pick a topic and have no one show up. At Addgene, we wanted to think more closely about the wealth...: Discussing next-generation sequencing at the Addgene bioinformatics journal club! What happens during...figure. Some follow the same figure by figure organization with a different person responsible for each...messaging but we as scientists often prefer data. At Addgene we rely on polls and surveys. A mix of closed (...most likely the primary drivers of frequency. At Addgene, we opted to start with one per month. When to ...journal clubs should be well planned and carefully organized. Most importantly, they should be regularly assessed...own journal club! Additional resources on the Addgene blog Read our blog posts centered around scientific...

...analyst, I negotiate with global organizations that partner with Addgene. What are the most important issues...training in a variety of transferable skills. At Addgene, we've set out to fill this gap by highlighting...with your colleagues within and outside of your organization. In my non-academic roles, some examples of ...issues for our partners and how can Addgene work with them to ensure we create a win-win situation? Learning...Richard D. Lewis Additional resources on the Addgene blog More from the transferable skills guide: ...management Managing a team Leadership Resources on Addgene.org Check out our careers page Watch our career ...

...’re bringing you a brand new third edition of Addgene’s CRISPR 101 eBook. This new edition of the eBook...Genome Editing: Modifying the DNA sequence of an organism’s genome usually for a research or applied goal...CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce either a gRNA, a nuclease, or both. Applications...

... & Small Molecule Fluorophores. Addgene Blog. https://blog.addgene.org/better-dyeing-through-chemistry-small-molecule-fluorophores... is now the most popular self-labeling tag in Addgene’s collection. CLIP-tag: Also in 2008, Kai Johnsson...Various Many Complicated   Applications Organic dyes are typically both brighter and more photostable...at a price, though. Purchasing lots and lots of organic dyes or specialized ligands is more expensive than...are the most popular self-labeling enzymes in Addgene’s collection, so you may be able to get a plasmid...ligands brings up bad memories of undergraduate organic chemistry lab, don’t worry – many SNAP-tag and ...10.1021/acs.biochem.1c00258 More resources from Addgene Better Dyeing Through Chemistry Illuminating Choices...

... also find information on using Addgene’s website, the latest Addgene news, and awards that we offer there...Say hello to the new Addgene blog! Same URL, same great articles to help you with your experiments, but...with over 600 articles, we needed a better way to organize and present the vast collection of material so...articles you want to find without even leaving the Addgene blog! Just click on the search bar at the top of...Be the first to know about new articles on the Addgene blog by subscribing to instant alerts below.   ...author pages, where you can read more about the Addgenie behind the blog post and find more of their work...interview. Get in touch by emailing us at blog@addgene.org or tell us in the comments below. We’ll track...

... a wide range of organisms and cell lines. In C. elegans, one of the model organisms which I use to study...perform pha-1 co-conversion are available through Addgene. In 2019, Farboud et al. described another similar...To see more CRISPR tools for C. elegans, visit Addgene’s C. elegans CRISPR plasmids page. Future perspectives...methods – could greatly streamline editing in other organisms and systems, allowing rapid progress in a wide...about genome editing with CRISPRs? Check-out Addgene's CRISPR Resources Learn about gRNA design and...

...culture that will hopefully help you keep a well organized lab and contamination free environment for successful...many times and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage...consistent growth rate. For every minute spent organizing, an hour is earned (Benjamin Franklin)! It’s ...Second  Edition), 2012. Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian...should keep in mind that UV light only kills microorganisms that it strikes directly. UV light exposure..."Ostreopsis cf. ovata induces cytoskeletal disorganization, apoptosis, and gene expression disregulation...Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your research Find...

...fluorescent when ectopically expressed in most organisms. Furthermore, fusing FPs to another protein usually...Additionally, many other FPs have been found in other organisms. Blue 424 - 467 nm Cyan 474 - 492 nm Green...More examples of these biosensors can be found at Addgene. Split FPs – some FPs (e.g. GFP, Venus) can be...More Helpful Websites & Resources: FP guide at Addgene Interactive Visualization of Fluorescent Protein...

... structures give the cell its shape, organize cellular organelles, and are necessary for movement and ...of your protein of interest with known markers. Addgene has assembled a collection of fluorescent fusion... Plasmid Collections Fluorescent Proteins Organelle Markers Fluorescent Proteins: Subcellular Localization...fusion proteins that are targeted to various organelles or subcellular structures. You should fuse your protein...General Structures The cell contains a variety of organelles and subcellular structures each with their own...table below to find markers of general cellular organelles and structures. ID Plasmid Structure Targeting...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...experiment? What if after receiving the plasmid from Addgene you could directly start your digest, PCR, or transformation...collection is highly diverse in terms of target organisms (from bacteria to mammals), cloning techniques...Suggestion Form.  Additional resources on the Addgene blog Browse blog posts from our Plasmids 101 blog...the various ways to quantify DNA Resources on Addgene.org Learn about our other beta projects Read our...

...Developing Addgene’s Brand and culture Chapter 7 - Building Addgene as a global organization (now with ...Resources from the Addgene Blog Addgene's a nonprofit? Get an Inside Look at Addgene's NGS Quality Control... In this episode of the Addgene podcast, we sit down with Addgene’s Founders - Melina Fan, Ken Fan, and...interview with Addgene's founders Podcast breakdown Intro Chapter 1 - Who are Addgene's Founders? Chapter...Chapter 2 - The process of founding Addgene, where the idea for Addgene came from and how the idea got off...current roles at Addgene and beyond - Ginkgo, Tufts Launchpad, and more Chapter 10 - Addgene's Future, the ...efforts at Addgene as the Chief Financial Officer and Chief Operating Officer. Prior to Addgene, Kenneth...

...Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s incredibly important for scientific...distribution and disseminate knowledge around the globe. Addgene’s progress in international distribution As you ...are making great progress toward spreading the Addgene community into developing regions like South America...the countries that have requested plasmids from Addgene since our inception in 2004. In the near future...to more collaborations with Seeding Labs and organizations like it. Additional Resources Find out how...SeedingLabs or on Facebook Additional Resources on the Addgene Blog Learn About Our Recent Visit to China Read... Institutions that Deposit their Plasmids with Addgene ...

... produce all of the differentiated cells in an organism, including the embryonic and extraembryonic cell...quantities in specific “stem cell niches” found in many organs in the adult human body. These generate specialized...PMCID: PMC3347549. Additional Resources on the Addgene Blog pCXLE Toolkit Stem Cell Models for Disease...Differentiate the Stem Cell Field Resources on Addgene.org Visit Our Stem Cell Page Find Plasmids for Reprogramming...