...development has caught up with it. The peer review process has been challenged with many closely spaced CRISPR... are trying to solve, and finally how we might proceed in the future. When we think of designing experiments...These functions help speed up the sgRNA design process by removing repetitive tasks and/or by providing...providing features to help with the post-design process such as primer and plasmid design. Examples:  “multiplex...

...Transformation grade DNA  Transformation is the process of inserting exogenous DNA in a microorganism. ...contaminants that have been used during the purification procedure. Good indicators of DNA purity are the absorbance...transfection grade DNA. Transfection is the process of inserting an external plasmid in mammalian cells...

...  I applied to Addgene and began the interview process with Amy, who let me know in March that I successfully...crunch and knew that if we were to restart the procedure we would not have enough time. During the following...This report shared my work by breaking down the process of each day, my background, observations, etc. ...

... Addgene has no investors or stock. All of our proceeds go into running the repository and serving scientists...curious what specifically drives the decision making process at Addgene, just look to our core values. These...deposit and request plasmids (via web interface, processes, distribution, and customer service) Addgene provides...

...Award highlights TTOs that accelerate science by processing agreements quickly! If your TTO receives a Golden...transfer professionals: Describe to your TTO how unprocessed agreements slow down your research; provide specific...all future requests/deposits. This reduces the processing time of request MTAs and deposit agreements to...

... What is a transduction?  Transduction is the process of transferring foreign nucleic acid into a cell.... Transductions are similar to transfections, processes which introduce foreign nucleic acid into cells...   Figure 1: Transduction to expression process. A viral particle enters a host cell, enters the...

...Autophagy (Greek for “self-eating”) is a process by which cytoplasmic material, including organelles,...lysosomes for degradation. Autophagy is a dynamic process which involves autophagosome synthesis, delivery...inherent limitations because it turns a dynamic process into a static measurement and it provides limited...

...hike in. Navigating challenges: collecting and processing environmental samples Since the high temperatures...samples at the hot spring. Hiking all day and processing samples by night was fun but also very tiring...And it was exciting – one night while trying to process samples at the picnic table before dark, a bison...

...production process while still ordering viral vectors. Virus production can be a frustrating process that requires...developing ourselves as a knowledge base for the processes of viral production and testing so that researchers...

...background and/or connections to even begin this process. I am the cofounder and CEO of miLab, a Boston-...innovative ideas in all facets of the entrepreneurial process in order to develop those ideas into commercial...people for guidance.  Before even beginning the process of launching a company, you need to determine whether...

...and effective. We do that through a standardized process of good research practice: computer simulations...’s experiments prove otherwise, the scientific process does seem to be working. I would argue that one...He and the CRISPR Babies is that the scientific process, as imperfect as it may be, does work when it is...

... is to quickly generate a single cell line and proceed to use it for experiments.” - Alex Chavez, researcher...scientists, producing lentivirus is not always an easy process. The cloning capacity of lentiviral vectors (~8.5... genes involved in a wide variety of cellular processes. While these are widely requested and incredibly...

...fact, precise light-based control of biological processes is highly useful in other fields, including synthetic...to directly activate and inactivate biological processes. Chemical effectors are suboptimal for a number...intensity and wavelength can be used to study dynamic processes. Conversely, varied input patterns may also be...

...polymerize, allowing the slower exonuclease activity to proceed. By including only one type of dNTP in the reaction... can decide exactly how far the T4 exonuclease proceeds: at the first occurrence of the chosen nucleotide...: Schematic of SLIC. To start the SLIC cloning process, a gene of interest is PCR amplified to add 5′ ...

...easier with more perspectives contributing to the process Senior people (of which there are never enough,...during the meeting.  Scientists do better with process, so don’t just get together and chat. Have an agenda...started. Have regular meetings. Experiment with the process. Have each person write a Development Plan. Set...

...scientist at Addgene. Cloning can be quite an arduous process. The PCR could fail to produce a product, the transformation...can go wrong! With all the steps in the cloning process, there are also many ways to troubleshoot the cloning...Addgene. In the lab he develops new assays and processes. Outside the lab, he enjoy the outdoor in all ...

... inserting electrodes into the brain. But this process is time consuming and expensive. Thus scientists... to spatially and temporally control cellular processes in vivo. Many photoactive proteins exist in nature...interactions can control subcellular localization processes in vivo. They deposited the resulting optogenetic...

...effect of protein synthesis and degradation. These processes control the abundance of proteins in cells. Homeostatic...incorporation of puromycin into peptide chains." Proceedings of the National Academy of Sciences 51.4 (1964...puromycin of amino acid incorporation into protein." Proceedings of the National Academy of Sciences 45.12 (1959...

...These each have different goals, formats, and processes, and they provide different amounts of access ...journal-based peer review is usually a confidential process among a few individuals.  Early-career researchers...errors, with a lack of transparency in most review processes. Preprints and their feedback, in contrast, are...

...the two strands are ligated, resulting in the reciprocal crossover event. This event can have three general...the stop codon is excised, and gene expression proceeds. One popular lox-stop-lox plasmid is from Tyler... remove the marker after the initial selection process. By floxing the selection marker, Cre can be used...