...certain number of days and tracking average transit time for each courier we contracted with so we can always...Culture Collections Lentiviral Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial ...

...cargo Optogenetic proximity labeling and TransitID Tracking cellular history with protein ticker tape Teaching... bioRxiv – doi: 10.1101/2023.02.07.527548.    Tracking cellular history with protein ticker tape By Brian...et al. (2023). Time-tagged ticker tapes for intracellular recordings. Nature Biotechnology, 41(5), 631...

...General expression mRNA Tetracycline response element promoter Inducible with Tetracyline or its derivatives...promoter with low basal activity and several tetracycline operators. Transcription can be turned on or...

...useful to avoid such procrastination: Remove distractions: Probably the easiest way to do this is to leave...internet and all it’s enticements are far too distracting. (As a side note, I’d recommend the book, “Bored...more fantastic reasons to remove yourself from distractions every once in awhile.) Reframe the task in a...

...thrive in laboratory settings. One of its most attractive features is its stability and easily obtainable...ability to regenerate, the axolotl has several attractive characteristics that make them an ideal model...for human disease research. One of their most attractive features are their eggs. Frog eggs are large,...

... signal is boosted and enables single molecule tracking in living cells. The HA frankenbody is an HA tag...expressed on T cells to make CAR T cells. The extracellular scFv portion of the receptors recognizes an ...an antigen of interest while the intracellular T cell receptor portion of the CAR facilitates signal transduction...

...like lamellipodia or filopodia formation, cell contraction, or other morphological changes, depending on...tools to control formation of lamellipodia and contractile stress fibers, and recently developed a full ...selection in mammalian cells used here could be an attractive strategy for engineering other enzymes. Conclusion...

...therapeutics, which is also gaining a lot of new traction lately (1, 2), depends on knowledge of the structure...storage. After overnight growth, scrape up, pool and extract plasmid DNA directly from the colonies. Transform...solution containing your favourite bacterial protein extraction reagent (BPER from Thermo works well) with some...

...microglia. This property makes the lentivirus an attractive choice for a gene transfer vector. Lentiviral...used inducible lentiviral vector system is the tetracycline (Tet)-regulated system. You can choose either...promoter in your transfer vector. In the absence of tetracycline or a derivative such as Doxycycline (Dox), the...

...culture through various sources that are difficult to trace. These include the laboratory personnel, the serum...mycoplasmas, contamination by other bacteria or extracellular fluorescent signals produced by these bacteria...fluoroquinolone ciprofloxacin, ciprobay, zagam, baytril, tetracycline, etc. are available for mycoplasma removal from...

...the sensitive FLEx-split TVA receptor used for tracing neural pathways was 8.5 x 10e10 GC/mL. The lot ...Adeno-Associated Viral Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678. https://doi.org...Jin L, Lea NE, Wickersham IR (2020) Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations...

...By generating PA-FPs fused to intracellular proteins, scientists can track single protein localization,...Photoactivatable GFP tagging cassettes for protein‐tracking studies in the budding yeast Saccharomyces cerevisiae...

.... Other dyes may prove challenging to stain intracellular compartments in in vivo model systems, so extra...green, red, and far-red dyes is important for intracellular fluorescence live imaging ​​(Hayashi-Takanaka...fission, fusion, biogenesis, and mitophagy, can be tracked using dyes like Rhodamine 123 (R123) (Liu et al...

...normal kit protocols.  Yeast plasmids – If you’re extracting plasmids from yeast, instead of bacteria, a few...kits — there are many more kits out there for extracting DNA from tissues, blood, virus, or other samples...samples, for PCR and gel cleanup, and for extracting RNA! (That’s a blog for another time, however!) Where...

... separately inducible by L-arabinose and anhydrotetracycline (aTet), respectively. pMAZ-SK contains an...et al., 2015). pCRISPomyces-1 includes Cas9, a tracrRNA, and a CRISPR array, while pCRISPomyces-2 contains...presence of the inducer, in this case anhydro-tetracycline (aTc). By cloning in two tandem copies of the...

...barcodes and scanners to keep track of reagents in your lab. With barcode tracking, you will always be able...

...Rabies and Neuronal Tracing” blog post! You can also browse tools for AAV tracing, made from rabies plasmids...

...Rabies and Neuronal Tracing” blog post! You can also browse tools for AAV tracing, made from rabies plasmids...

...of support and structure, prevent science from attracting and keeping diverse talent in science related...prioritize ongoing work.  If we’re going to continue attracting and launching science talent we must ensure that...

...important of those is to always look closely at the trace file (or chromatogram) of the sequencing results...sequence (no Ns in sight). If you never looked at the trace you would be happy. But look closer, the overlapping...