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We narrowed to 255 results for: tre promoter

Showing: 181 - 200 of 255 results

  1. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...its paralog RC2H2 are RNA binding proteins that promote RNA decay and their upregulation is correlated ...little was one minute BASU generated a strong streptavidin signal (~250-fold enrichment) when compared ...

  3. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...Program at Harvard Law School. He has continually promoted the cause of increasing data collection about ...Symposium Boston 2015 you can register here or live stream the event on October 22nd and 23rd here. Many ...

  4. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...orientation. This allows for multiple DNA components (promoters, genes, terminators, etc) to be assembled in the...ligation process is close to 100% efficient. Another strength of Golden Gate cloning is its scalability. Unique...

  6. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ... had multiple integration events. In addition, promoter choice can also impact transgene expression. The...transduced with serial dilutions of a viral prep, treated with the antibiotic, and colony-forming units quantified...

  7. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...surprise since the PAM flexibility could in theory promote off-target binding. The activity of SpRY was validated...precise targeting (give or take a bp or two upstream or downstream) for most gene targeting experiments. Between...

  8. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...decreases. Hypermethylation of CpG islands in the promoter region of tumor suppressor genes can repress their...study and treat complex or multifactorial diseases, enabling faster diagnosis and treatment and improving...

  9. PhD Applications After COVID

    Type
    Blog Post
    ...universities stressful or simply not feasible. Through the virtual systems created and promoted during COVID...fit for you without the financial and logistical stress that accompanies traveling for campus visits. This...have to worry about traveling during an already stressful time, and I was more relaxed during both sets ...their end. Though the quick changes in plans was stressful in and of itself, the flexibility allowed through...using the virtual world to your advantage can strengthen your application and help you decide if a program...

  10. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...complexed with a gRNA that directs them to the promoter of a gene of interest, they have been shown to...conventionally used SpCas9 (derived from the bacterium Streptococcus pyogenes). Consequently, fewer genetic tools...complex. When this complex is directed to a target upstream of the transcriptional start site (TSS) of a gene...competent to cut DNA. When directed to a site downstream of the transcriptional start site of a gene of...

  11. dTAG - You're it!

    Type
    Blog Post
    ... al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 2017. 171(7):1573-1588.... Test dTAG molecule toxicity in new cell lines Treat parental cells (or cells expressing a control --...

  13. Hot Plasmids - October 2020

    Type
    Blog Post
    ...express hybrid Cas9-Cas12a gRNAs under a single U6 promoter. The paralog and dual-targeting hybrid gRNA library...Addgene Sc++ is a codon-optimized, engineered Streptococcus canis Cas9 nuclease that has a broad 5′-NNG-...

  15. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...gene expression in C.elegans. In this system a promoter controls the expression of a GAL-4 driver that...that can bind to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In ...

  16. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...walk into any supermarket now without seeing a promotion for antibiotic-free, farmed meats. We can focus...antibiotics. When we use antibiotics to treat infections, we are treating microbes with compounds similar to...will be more difficult to treat later. A similar situation can occur if you treat a bacterial infection with...more different classes, or types, of drugs used to treat them. Very often, this is what it means to be a ...often very difficult and very time consuming to treat. Learn How Antibiotic Resistance Genes Are Used ...antibiotics in livestock farming Lengthy courses of treatment with antibiotics These scenarios expose bacterial...the population and making them more difficult to treat in the future. Patients not finishing the whole...

  17. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...activity Regulating Acr proteins by inducible promoters, light, or small molecules could achieve rapid...meningitidis (Nme) (Pawluk et al., 2016). While Streptococcus pyogenes Cas9 (SpyCas9) is the most used and...system. 5. Phage therapy Phage therapy uses phage to treat bacterial infections as an alternative to antibiotics...

  18. Zhang Lab CRISPR Page

    Type
    Collection
    ...Truncated MeCP2 promoter-driven SpCas9; for neuronal expression 60958 : PX552; U6 promoter-driven; for sgRNA...tyroxine binding globulin (TBG, PX602 [#61593] ) promoter, and a U6-driven single guide RNA. The vector ...codon-optimized SpCas9, driven by the truncated MeCP2 promoter (pAAV-pMecp2-SpCas9-spA) for expressing Cas9 in...FA, Hsu PD, Lin CY, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang...CRISPR-Cas9 complex. Konermann S*, Brigham MD*, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

  19. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...on the Addgene Blog Origin of Replication The Promoter Region – Let's Go! Browse All of Our Plasmids ...in cells treated with Plasmid A is similar to the expression level of Gene X in the untreated cells—but...be determined empirically.  Negative controls: untreated cells, empty vector control, and non-targeting...is observed). In any plasmid-based experiment, untreated cells should be included as these provide the ...

  20. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...an enhanced pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  Figure 1...dynamics of the motor protein kinesin-1, the Twelvetrees Lab took advantage of the self-labeling tags ...Turner, E.D., Abdelhamid, M.A.S., Craggs, T.D., & Twelvetrees A.E. (2024) Kinesin-1 is highly flexible and ...
Showing: 181 - 200 of 255 results