...others are dubious enough that they don’t give it a try. Optimization Why is GCE so complicated? Well, while... Expansion: A Brief History and Perspective. Biochemistry, 60(46), 3455–3469. https://doi.org/10.1021/...

...open-source modular optical toolbox openUC2 are trying to overcome this hurdle by taking these limitations...

...example where a selection would work is if you are trying to identify genetic conditions which convey resistance...

...Resources on the Addgene Blog Learn How Scientists Are Trying to Shape the Future of Research Learn How Notions...

...originally came about as Bhang and co-workers were trying to develop a way to quantify the take rate of mouse...Novartis because she wanted first-hand experience in industry research. When she came to Novartis, she was happily...

...toolbox you are putting together?  Voigt: We’ve been trying to get everything to the scale of building synthetic...is that Cas9 is toxic. When cells carry CRISPR circuitry, it can slow their growth. Something has to be...program you can imagine. The Apollo 11 mission circuitry that took a spaceship to the moon was built on...We did demonstrate that you can use the CRISPR chemistry for a NOR gate. But there are a couple of problems...

...autophagy of your protein of interest. Are you ready to try out Rosella? Both the cytosol-targeted and mitochondria-targeted...

... parents. If both of the mutations that you are trying to cross are located on homologous chromosomes ...

...could bind not only the endogenous proteins you are trying to block but your primary antibody as well.  Next...

...Other Conjugates to Know It would be overwhelming to try to cover all of the possible antibody conjugates ...like fluorescence microscopy, flow cytometry, or spectrophotometry. The range of fluorophores available...typically used in microscopy-based assays like immunohistochemistry, though they are also a common choice for...al., 2017). Metal isotopes are useful for mass cytometry (Bendall, et al., 2012). And, you can also conjugate...purposes.  Coupling up - antibody conjugation chemistry  Whatever the conjugate, the molecules must somehow...Chattopadhyay PK (2012) A deep profiler’s guide to cytometry. Trends Immunol 33:323–332. https://doi.org/10.1016...

...role at Addgene is “Words are important!”  If you try any of these approaches in your lab or workplace,...

...have many levels of complexity, especially when trying to explore the links between genetics and anatomy...

... next? First, check the submission deadline and try not to panic if it's due in a few days, weeks, or...

... a sample of possible dilutions. You may want to try higher/lower dilutions depending on your downstream...

... purposes. What do I do if my PCR isn't working? Try adding 1µl of 25mM MgCl 2 and/or 1µl DMSO to each...

...efficiency. Before constructing your own FRET probe, try searching PubMed for articles describing the FRET...

...depends on several factors: The protein you are trying to express How much protein you need   Your plans... its use as a model organism in genetics and biochemistry. When using S. cerevisiae, researchers often...

...can grow in spite of a hostile Penicillium fungus trying to kill them off. While antibiotic resistance-mediated...

...site. Or, if you are visiting a local classroom or trying to explain your science to the general public, ...

...getting sub-optimal plasmid yields from your prep, try our tips below! How to optimize your plasmid prep...