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We narrowed to 535 results for: val

Showing: 181 - 200 of 535 results

  1. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...created your fusion protein, it's important to validate its function relative to the untagged, wild-type...wild-type protein. You may, for example, validate that the fusion protein localizes properly by comparing the..., and John H. McDonald. "A practical guide to evaluating colocalization in biological microscopy." American...

  2. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ... that, when expressed, normally encourage cell survival, growth and proliferation, while tumor suppressors... those of the pancreas and colon. Despite the prevalence of Ras mutations in a variety of cancers, Ras...different mutant versions of Ras that are most prevalent in human cancers, and synthesizing compounds to...

  3. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...some items vary, for example, plasmid kits are avaliable for less than than it would cost to buy each plasmid...DH5a, but others may require strains such as ccdB survival (for Gateway plasmids).  We also like to store...plasmid, and which experimental systems it was validated in.   Please keep in mind that all of the information...

  4. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...Co-expression of multiple genes is valuable in many experimental settings. To achieve this, scientists...peptides instead of an IRES when stoichiometrically equivalent levels of expression are required, we should ...construct that expresses multiple unique genes at equivalent levels upstream of an IRES fluorescent reporter...

  5. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...experiences and that it often provides incredibly valuable insight into one’s work. As a community, we must...are designed specifically to allow scientists to evaluate how small changes in vector administration can...enormous amount of data potentially available to evaluate AAV vector performance. Yet, most of the technical...

  6. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ... disease and not another genetic disease more prevalent or life-threatening (cystic fibrosis, hemophilia...trial started, and 10 more years to obtain FDA approval and make history in the form of Luxturna™. A ...manufacturing of viral vectors to support trials for prevalent diseases - to name a few. CRISPR-based tools ...

  7. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...generally referred to as Antigen Retrieval. I chose not to get into antigen retrieval here because it’s not quite...proteins. Cross-linking is a chemical reaction that covalently joins two molecules and it is great for preserving...

  8. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...from that country.   While written resources are valuable, there does come a time when a picture is worth... favorite measure of our educational resources’ value is feedback from the researchers who use them. The...website survey on addgene.org. Currently, we’re evaluating our depositor process to identify and reduce ...

  9. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...molecules from a variety of sample types (Engvall 1971). Scientists value ELISAs for their accuracy, specificity...the level of target antigen in a sample. While Engvall et al. originally developed the ELISA assay to ...

  10. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...pooled lentiviral libraries. A) Amplification, validation, and lentiviral generation of a pooled library...complexity of pooled libraries, NGS is the best way to validate your amplified library and verify that you have...lentiviral production, transduction, screening, and validation of CRISPR libraries. The Genome Engineering using...

  11. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... to share these invaluable reagents with the scientific community. Note: Cary Valley and Mary Gearing... Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment Check Out Our CRISPR...

  12. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ... foster a culture of inclusivity. Work with and value people who think differently from you and you will... share ideas. Ideas had over lunch are just as valuable! In 2019, Addgene implemented a company-wide New...come up with any ideas obviously worth pursuing, evaluate the “bad” ones. Bad ideas are easier to work with...

  13. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...position requirements. For example, a KDEL ER retrieval sequence only functions at the extreme C-terminus...will fold and that the fluorescence pattern is equivalent to the distribution of FP-fusion proteins. Our.... Pubmed Central PMCID: PMC2150754. Resources Avaliable at Addgene Read Our Blog Post on Protein Tags...

  14. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...or disagreeable, you recognize their feelings as valid. Listening: In one of my favorite books, Michael... means the other party will feel punished or invalidated. Both compromise and arbitration might remedy...bringing this asset to your job, you can become a valuable asset yourself.     Fig 2: A diagram of...

  15. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ... https://doi.org/10.1016/j.celrep.2017.06.064  Carvalho LS, Turunen HT, Wassmer SJ, Luna-Velez MV, Xiao...Xiao R, Bennett J, Vandenberghe LH (2017) Evaluating Efficiencies of Dual AAV Approaches for Retinal Targeting.../mt.2009.280 Hirsch ML, Li C, Bellon I, Yin C, Chavala S, Pryadkina M, Richard I, Samulski RJ (2013) Oversized...

  16. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...anticipated restriction analysis results (left) and validation PCR followed by Restriction Fragment Length Polymorphism...introduced restriction site is also duplicated. Validating your mutagenized plasmids Once constructs containing...confirmed to be free of primer duplications), final validation is performed by sequencing functional regions...

  17. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...spectrometry suggest that phosphorylation is far more prevalent than previously appreciated. PhosphoSitePlus, ...as described below) provides researchers with a valuable tool for understanding the contribution of phosphorylation...glutamate which allows the system of interest to be validated first through the production of full-length protein...

  19. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ... about you. It’s worth taking time to honestly evaluate your own style of work and communication before...acknowledge good work, allocation of meaningless tasks, removal of responsibility, repeated reminders of blunders...lab for you is out there if you take the time to evaluate the options critically. How will it feel when ...

  20. Finding Your Perfect Job After University

    Type
    Blog Post
    ...tumour suppressor. This summer role was incredibly valuable because it was a set duration of only three months...previous experience working in Industry was really valuable; I had received all the training and experience... required a scientist for a 6 month project to validate some genomic changes, so this arrangement was ...
Showing: 181 - 200 of 535 results