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  1. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...human GPCRome. Nat Struct Mol Biol 22:362–369 . https://doi.org/10.1038/nsmb.3014 Paek J, Kalocsay M, ...Peroxidase-Catalyzed Proximity Labeling. Cell 169:338-349.e11 . https://doi.org/10.1016/j.cell.2017.03.028 Roth BL (2016...DREADDs for Neuroscientists. Neuron 89:683–694 . https://doi.org/10.1016/j.neuron.2016.01.040 Takeda S,...human genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016/s0014-5793(02)02775-8 Additional...

  2. Hot Plasmids - February 2022

    Type
    Blog Post
    ...Genes Cells. 2021. https://doi.org/10.1111/gtc.12893, Zhao et al. Nat Comm. 2019. https://doi.org/10.1038...domains.  Santilli, R.T., et al. Sci Rep (2021). https://doi.org/10.1038/s41598-021-96051-x Engineered...therapy.  Xiaoshu X, et al. Molecular Cell. 2021. https://doi.org/10.1016/j.molcel.2021.08.008. ...

  3. RNA Extraction Without A Kit

    Type
    Blog Post
    ...Biophysica Acta (BBA) - Enzymology 341:484–488. https://doi.org/10.1016/0005-2744(74)90240-x Chirgwin ...enriched in ribonuclease. Biochemistry 18:5294–5299. https://doi.org/10.1021/bi00591a005 Chomczynski P, Sacchi...Extraction. Analytical Biochemistry 162:156–159. https://doi.org/10.1016/0003-2697(87)90021-2 Chomczynski...twenty-something years on. Nature Protocols 1:581–585. https://doi.org/10.1038/nprot.2006.83 Additional resources...

  4. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...the National Academy of Sciences 104:5163–5168 . https://doi.org/10.1073/pnas.0700293104 Field J, Nikawa...epitope addition method. Mol Cell Biol 8:2159–2165 . https://doi.org/10.1128/mcb.8.5.2159 Galvan A, Raper J...DREADD s in monkeys. Eur J Neurosci 50:2801–2813 . https://doi.org/10.1111/ejn.14429 Kimple ME, Brill AL,...Purification. Current Protocols in Protein Science 73: . https://doi.org/10.1002/0471140864.ps0909s73 Additional...

  5. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...and Environmental Microbiology, 67(8), 3440–3444. https://doi.org/10.1128/AEM.67.8.3440-3444.2001 Fogarty... in the human gut. Cell, 187(5), 1206-1222.e16. https://doi.org/10.1016/j.cell.2024.01.039 Garcillán-Barcia... Plasmid classifications. Plasmid, 126, 102684. https://doi.org/10.1016/j.plasmid.2023.102684 Shareck,...Critical Reviews in Biotechnology, 24(4), 155–208. https://doi.org/10.1080/07388550490904288 Additional Resources...

  6. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...hours.  Sheets, MB, et al., ACS Synth Biol. 2020. https://pubs.acs.org/doi/10.1021/acssynbio.9b00395 O’Brian...’Brian, SP, et al., Biotechnol J.  2014. https://pubmed.ncbi.nlm.nih.gov/24390935/ Hypercompact genome...from CRISPR-Casɸ. Pausch et al., Science 2020, https://pubmed.ncbi.nlm.nih.gov/32675376/ mGold, the ...molecules.  Lee, J. et al., Science Advances 2020. https://doi.org/10.1126/sciadv.abb7438 The CRISPR corner...

  7. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...HEK 293 cells. The FASEB Journal, 16(8), 869–871. https://doi.org/10.1096/fj.01-0995fje. Tan, E., Chin, ...Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389/fbioe.2021.796991. Urabe, M., ...2 vectors. Human Gene Therapy, 13(16), 1935–43. https://doi.org/10.1089/10430340260355347. Vaughn, J. ...lepidoptera; noctuidae). In Vitro, 13(4), 213–217. https://doi.org/10.1007/bf02615077....

  8. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ... Cell. 2020. https://doi.org/10.1016/j.cell.2020.08.012  Starr et al Science. 2021. https://doi.org/10.1126...Preger et al., Journal of Biological Chemistry 2020. https://doi.org/10.1074/jbc.RA120.01289     Novel protein...biosynthetic pathways.   Cheah et al., bioRxiv 2021. https://doi.org/10.1101/2021.01.30.428974   The CRISPR...

  9. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ... BLAST: ExPASy - http://www.expasy.org/genomics/sequence_alignment EMBL-EBI - http://www.ebi.ac.uk/services...services http://www.ebi.ac.uk/Tools/webservices/#multiple_sequence_alignment_msa http://www.ebi.ac.uk...

  10. DNA Purification Without a Kit

    Type
    Blog Post
    ...Purification Method Using Glass Syringe Filters. PloS one. https://doi.org/10.1371/journal.pone.0007750  Vogelstein... of the United States of America, 76 2, 615-9 . https://doi.org/10.1073/pnas.76.2.615  Zhou, Y., Zhang...Purification and Gel Extraction Kits. Scientific Reports. https://doi.org/10.1038/s41598-018-30316-w  Zou, Y., Mason...and microbes in under 30 seconds. PLoS biology. https://doi.org/10.1371/journal.pbio.2003916...
Showing: 101 - 110 of 246 results