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We narrowed to 125 results for: lenti crispr

Showing: 101 - 110 of 125 results

  1. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...Has Been Used in Cancer Lineage Tracing Read How CRISPR Can Be Used in Genome-Wide Screening Browse 28 ... from other researchers, and cloned them into lentiviral vectors to generate a kit which is now available... cloned all the cDNAs into a single consensus lentiviral expression vector and attached a DNA barcode ...

  2. Neurodegeneration Research Collection

    Type
    Collection
    ...Dhekne et al. Elife. 2023 Oct 24. See More CRISPR Tools Find CRISPR pooled libraries available from Addgene...neurodegeneration research. Disease Info Plasmid Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors...about the blood brain barrier integrity, and using CRISPR screens to understand more globally how neurons.... Target neural oxytocin receptors using an AAV-CRISPR/Cas9 strategy for gene editing across divergent... Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian...plasmid pooled libraries . New and Noteworthy: Use a CRISPRi system to target alpha-synuclein. Sastre et al....

  3. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...It's a human CRISPR inhibition library that inhibits over 18,000 human genes and is used for genome-wide... of the AAV and lentiviral preps we have released in the last few months: Lentiviral preps for expressing...plasmids into lentiviral preps to speed up COVID-19 research even further. The following lentiviral preps are...few weeks. CRISPRi pooled library The Dolcetto pooled library is now available as a lentiviral prep. It'... GFP dependent Cre and FLPo And more!  New lentiviral preps SARS-CoV-2 protein expression The Krogan...2xStrep-IRES-Puro: Expression of the SARS-CoV-2 ORF3a Lentiviral preps for expressing the E protein (pLVX-EF1alpha-SARS-CoV...genome-wide inhibition screening. Find the Dolcetto lentiviral prep here Control AAV Control AAV allow researchers...

  4. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular ... may recall our first 20th anniversary story on CRISPR, where we dove fairly deeply into the different...

  5. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...integrate (unless you used a transposon system, CRISPR targeted strategy, etc.) and episomal expression...perform the transduction with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired DNA to... some viruses integrate into the host genome (lentivirus and retrovirus) while others are non-integrating...Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene Viral Services...

  6. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ... CRIPSR User (By a 1st Time CRISPR User) Tips for Titering Your Lentivirus Preps Additional Resources...different types of tubes: the ones we use to store lentivirus and the ones we use to store AAV. Our results...effect on the pH of the samples stored in our lentivirus tubes (Fig. 1). However, once thawed, there was... lentivirus...

  7. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...the recent release of the Spanish version of our CRISPR 101 eBook and the upcoming release of the second...comprehensive transcription factor library, in lentiviral vectors. At 3500 plasmids, this represents the...

  8. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ...downregulation via RNAi or gene editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency...brain region of interest. AAV or lentivirus?  Both AAV and lentiviral vectors are used for intracerebral...wider diffusion is required. On the other hand, lentiviral vectors are superior packaging vectors for larger...astrocytes, and AAV4 targets mostly astrocytes. Lentiviral vectors are pseudotyped in most cases with VSV-G...injection. Find more details about choosing AAV or lentiviral vectors in our previous blog posts.  Viral vector...1013 vg/ml (vector genomes per milliliter) and lentiviral vectors around 108 IU/ml (infectious units per...

  10. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...Therapeutics and is an Addgene depositor. KSQ uses a CRISPR-Cas9 platform to systematically test the function...Harvard Medical School, he developed the first lentiviral microRNA-based system for polymerase II-regulated...
Showing: 101 - 110 of 125 results