...  HuBMAP Consortium Healthy human cells Imaging (IHC, ICC, IF, etc.)  Data repository From ... Knowledge-Base  Any IBEX multiplex tissue imaging Data repository See blog post mAb3D Atlas...

...addgene.org to learn more. A great submission includes: Images that clearly show viral spread and cellular tropism... a functional vector such as a channelrhodopsin Images showing the injection site as well as the target...

...of 304 open access publishers. By the time the magazine went to press, no less than 157 of those journals...let us know your favorite ways to share science. Image credit: Judy Breck, via flickr  ...

...cells for ctrA gene expression using various dCas9. Image adapted from Guzzo et al., 2020. Caulobacter ... Figure 2: The components of Casilio-ME1. Image from Taghbalout et al., 2019. Fusing epigenetic...

...ColabFold (Mirdita et al., 2022). B, Representative images of HEK293T cells and neurons expressing nLightG...for Me?  In Living Color: The Skinny on In Vivo Imaging Tools Resources on Addgene.org Find Biosensor...

...highlights written as 8 tweetable facts. Note: The images used in this post were created using data from ...scientific domains tweeted by biologists were: Nature, Sciencemag, Wiley, Cell and Scientific American Figure ...

...of 1.2 per megabase in mammals), leading to DNA damage and developmental aberrations. In multiple systems...10.1038/ni0707-665. PubMed. Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells. ...

...source of mouse-derived single-domain antibodies. Image created in BioRender and adapted from Xu, et al..../fimmu.2017.01589 Jin B, Odongo S, Radwanska M, Magez S (2023) Nanobodies: A Review of Generation, Diagnostics...

...routinely deposited in Addgene!” As you can imagine, answering plasmid requests can be a time-consuming...researchers from far corners of the world. “It’s easy to imagine how hard it is to distribute thousands of plasmids...

...Corning 25-015-CI) 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...sample were loaded in wells B01-H01. As shown in the image (Figure 1) and table below, the concentration of... to the lower dilutions (blue). As shown in the image (Figure 2) below, the concentration of RPP30 positive...

... of producing fluorescent proteins and enzymes. Image from Moore et al., 2021.   This TX-TL system... genes known or suspected to be involved in DNA damage response and DNA repair.  A new CRISPR pooled library...

...experiments directly from Addgene. How Addgene is Reimagining Antibody Sharing Addgene is building our antibody...includes frequently cited antibodies for use in brain imaging (Andrews et al., 2019), validated in both western...

...bacteria will be stained red or pink, as shown in the image below. An example of Gram positive (purple...purple) and Gram negative (red/pink) staining. Image credit: Mark Perkins https://www.flickr.com/photos/occbio...

... more have been critical for pandemic research. Image from Reproducibility for Everyone. Case study...Comparative tropism, replication kinetics, and cell damage profiling of SARS-CoV-2 and SARS-CoV with implications...

...brightest resting fluorescence and can be used for imaging small neuronal processes (dendrites and axons)....maintaining high peak fluorescence) and is useful for imaging signaling activity in large populations of densely-labeled...

...of the most important steps in the design of an imaging experiment.  The choice is non-trivial, however...Choosing Your Fluorescent Proteins for Multicolor Imaging Resources on Addgene.org Browse all fluorescent...

...location of the ribosome, TRICK (Translating RNA Imaging by Coat protein Knock-off) instead takes advantage... M., et al. “Translation. An RNA biosensor for imaging the first round of translation from single cells...

...you have done fluorescent imaging, then you probably have experience using image analysis software. If you...

...are sensors available for other ions including magnesium, potassium, and zinc. These ion sensors can be...of them have even been expanded for multiplexed imaging for advanced monitoring of different factors, in...

... Figure 1: The SRIRACCHA reporter construct. Image from Wen et al., 2017. Once you’ve introduced...fluorescence.  Figure 2: The GEmCherry reporter. Image from Højland Knudsen et al., 2018. So how does...