We narrowed to 379 results for: Lor

1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   Blog Post

   ... apramycin, blasticidin, bleocin (zeocin), chloramphenicol, erythromicin, gentamicin, hygromycin, kanamycin...

2. Viral Vectors 101: Calcium Sensors

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   Blog Post

   ...trafficked and regulated. In this blog we will explore some of the most commonly used as well as newly...

3. Viral Vectors 101: Chemogenetics

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   Blog Post

   ...Roth's and Takafumi Minamimoto's lab called deschloroclozapine (DCZ) shows promise as a CNO alternative ...

4. Plasmids 101: The Wide World of Natural Plasmids

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   Blog Post

   ... on the Addgene blog as scientists continue to explore Mother Nature’s toolbox for new and exciting molecular...

5. Antibodies 101: Stripping and Reprobing Western Blots

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   Blog Post

   ..., respectively. Chromogenic detection produces colored precipitates that permanently stain the membrane...

6. Hot Plasmids and Viral Preps - January 2021

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   Blog Post

   ...specific tight affinity glutamate sensor from the Loren Looger Lab now available in AAV1. Get GFAP.SF-iGluSnFR.A184S-AAV1...

7. Early Career Researcher Toolbox: Finding Relevant Papers

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   Blog Post

   ...papers later. Pro tip Learn more about authors by exploring author pages (for example, Melina Fan’s author...

8. Cpf1 Update: Comparison to Cas9 and NgAgo

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   Blog Post

   ...insertion mediated by Cpf1-generated overhangs. Exploring the efficiency of these two processes will help...

9. Plasmids 101: Terminators and PolyA signals

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   Blog Post

   ... Julian Taylor-Parker...

10. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post

    ...levels of complexity, especially when trying to explore the links between genetics and anatomy, which are...

11. Plasmids 101: 5 factors to help you choose the right cloning method

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    Blog Post

    ...more than one right answer, so don’t be afraid to explore multiple approaches to find out which one works...

12. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    Blog Post

    ...cloacae, a bacterium that’s part of normal human gut flora. Guides targeted essential and non-essential genes...

13. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post

    ...cassette in one single viral particle. The earliest explorations of the use of AdV for in vivo CRISPR-Cas delivery...

14. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    Blog Post

    ...after a few washes, binding is detected using a colorimetric reaction. After the ELISA step, the narrowed-down...

15. Transferable Skills: Negotiation

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    Blog Post

    ...manager to gain experience in the area I want to explore while also ensuring that my work gets completed...

16. Viral Vectors 101: Voltage Indicators

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    Blog Post

    ... JFs can cross the blood brain barrier and many colors of JFs are available to swap into the system. Single...

17. Hot Plasmids: Winter 2025

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    Blog Post

    ... gene to confer resistance to the herbicide chlorsulfuron (Homo line 2) or wild type (WT), two weeks after...

18. Degrading DNA with Cascade-Cas3

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    Blog Post

    ...., Ramos, D., Hibshman, G. N., Wright, J. T., & Taylor, D. W. (2023). Structural snapshots of R-loop formation...

19. 3D Printing Meets CRISPR Cas9

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    Blog Post

    ...articles have been filled with impressive, full-color images of proteins. But they are still flat. To ...

20. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post

    ...knock-ins, but this possibility is still being explored. More broadly, Sakuma et al.’s use of MMEJ represents...

21. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    Blog Post

    ...State of Science could be a jumping off point to explore future rabbit holes. For the non-scientists in ...

22. Viral Vectors 101: The Retroviral Lifecycle

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    Blog Post

    .... PubMed Central PMCID: PMC355630 Faras, A. J., Taylor, J. M., Levinson, W. E., Goodman, H. M., & Bishop...

23. Uncovering Ancestral Bias in CRISPR Research

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    Blog Post

    ...Cancer Institute, and Koch Institute, where an exploration into the effects of ancestry and germline sequence...

24. A Guide to Starting Your Own Journal Club

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    Blog Post

    ...have even considered. These questions should be tailored to the group and format but should almost universally...

25. The Importance of a Fun Workplace: Company Culture at Addgene

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    Blog Post

    ...better together.I realize I may have painted a rose-colored-glasses-esque picture of how everyone is kind and...

26. Protocol - pLKO.1 – TRC Cloning Vector

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    Protocol

    ...contains: 10g tryptone 5g yeast extract 10g sodium chloride 15g agar Prepare LB agar solution by dissolving...

27. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    Blog Post

    ...effects of guide length on off-target cuts was explored using full-length (19-20 bp) or short (17-18 bp...

28. Save Time with Transient Plant Leaf Transformations

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    Blog Post

    ...cannot be studied in hairy roots, as functional chloroplasts and leaf specific cell types are missing in ...

29. Designing Your Chalk Talk for the Academic Job Interview

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    Blog Post

    ... fresh set of erasable markers. You’ll have the colors you want and you know they will work. Stabilize...

30. CRISPR-mediated Plant Base Editors

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    Blog Post

    ...base editing. They first created a defective chloramphenicol resistance gene (CamR) by introducing a point...