... and open source software, hardware, wetware, technology development, etc). Second, the potential hire...job satisfaction (especially for scientists and technical people) is linked to engagement and access to ...you would like to head for a career in pharma or biotech, this is one case where the topic of your research...

...visible part behind these changes: the backend technology that powers the new website search. Tackling ... resources, blog posts or reagent items.  The technology behind Addgene’s new search  Like millions of...PI labs and institutions. There are many more technical details that make our new search engine unique...

... sharing, there will always be an interest in protecting intellectual property. Figuring out that balance...year and we have a very dominant presence in the biotech sector in both patent law and general life science...Young, LLP, auditing insurance, finance and high-tech industries. He has earned both an MBA in finance...

...acids in the domain). Yan et al. from Arbor Biotechnologies found that RspCas13d accessory protein RspWYL1...functional in multiple species (read Arbor Biotechnologies' press release about their Cas13d deposit here...Addgene. Learn more about research at Arbor Biotechnologies. Additional Resources on the Addgene Blog:...

... creativity in employing a variety of cloning techniques and troubleshooting methodologies drew me to ...antibodies are commonly used in research laboratories to detect the different subclasses, but there are also secondary...use of subclass specific secondary antibodies to detect multiple targets with different mAbs in the same...

...biology techniques. What’s all the fuss? Well, ChIP’s complexities come not from the techniques used, but...immunoprecipitation (ChIP) is an extremely useful technique that provides insight into protein:DNA interactions...

...excitation by a laser, which is then read by a detector panel in the machine. Fluorophores are light sensitive...different signaling molecule, that all need to be detected in the same assay. This is one of the most complex...signal "spilling over" into another channel's detection range. Though you can compensate for this when...

... IgG2, IgG3, and IgG4. IgE IgE antibodies help protect against parasites but also play a role in allergies...secretions (e.g. tears, saliva, mucus) where it protects mucosal membranes. This isotype is most commonly... using monoclonal antibodies Read about other techniques that you can use antibodies for in our Intro ...

...heterotrimeric G protein complex that can be used to detect G protein activation via bioluminescence resonance...response in vivo (Paek et al., 2017). In this technique, the receptor fused to APEX2 catalyzes the biotinylation...are then identified with mass spec. Using this technique termed “GPCR-APEX” the Kruse lab was able track...

...fluorescent molecule. A higher brightness increases the detected signal and therefore high brightness is an important...brightness that does not fold well or cannot be detected in a microscopy setup has zero practical brightness...excitation wavelength, available emission filters, and detector sensitivity) and the biological system (temperature...

...Reversing the orientation of the direct repeat protects the (+) stranded lentivirus RNA from Cpf1-mediated... is one of the latest developments in CRISPR technologies.  It’s a simple and effective method with multiple...CRISPR–Cpf1 Asing a Single crRNA Array." Nature Biotechnology 35.1 (2016): 31-34. Pubmed PMID: 27918548. 2...

...features with gamma-retrovirus including genome architecture, host integration, and its association with ...Schambach A. Gammaretroviral vectors: biology, technology and application. Viruses. 2011 Jun;3(6):677-713...An Overview of the Application of Viruses to Biotechnology. Viruses. 2021 Oct 14;13(10):2073. DOI: 10.3390...

...gaps and add to them as techniques evolve. Blog posts get out of date as techniques and knowledge changes... useful information on scientific literature, technical skills, and experiences from thousands of laboratories...

...CRISPR technology has changed how scientists edit and control genes, but according to the Broad Institute's...activate essentially any gene,” she says. The technical leap from mutating and deactivating a gene or ... require access to next generation sequencing technology. These libraries are lentiviral in nature, ...

...to essentially any DNA sequence desired. This technology allows you to create a pair of designer proteins...on traditional homologous recombination. That technology had been used for decades to generate knock-out...That’s the revolutionary aspect: TALENs take a technology that was difficult for all but the most experienced...

...Biomedical Engineering, 2020. PubMed PMID: 31937940. Detection of neural activity using a red fluorescent genetically...encoded voltage indicators (GEVIs) are proteins that detect and output the voltage changes in neural activity..., has increasingly been recognized for its biotechnological applications including conversion of CO2 into...

... by the reliance on pH-based biosensors used to detect the maturation of neutral vesicles to more acidic...For further reading on fluorescent microscopy techniques, the first author of this article, Dr. Douglas...with classical gene overexpression and knockout techniques. Find the plasmids from Aranda-Díaz et al. Aranda-Díaz...

... an antibody that is directly conjugated to a detection substrate, such as a fluorophore, or a conjugated...do you know about the vendor and the vendor’s technical support? An antibody from a vendor that you use...frequently, with a responsive and knowledgeable technical support team, is a safer bet than one from a large...

...experiments that require multiple antibodies that each detect a different antigen. To pre-adsorb the antibodies...section), it may be advantageous if you are trying to detect a protein in low abundance or whose conformational...Overlooked benefits of using polyclonal antibodies. BioTechniques 65:127–136. https://doi.org/10.2144/btn-2018...

...it, his lab at the Massachusetts Institute of Technology has been “working on new experimental and theoretical... collision that’s taking place between CRISPR technology and synthetic biology, the tools he’s making ...all heading? Voigt: The top products out of biotechnology today are things like butanol - very simple ...

...updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology is constantly evolving. Variants of Cas proteins...be engineered to “armor” therapeutic phages to protect them from pathogenic bacteria’s CRISPR-Cas system... applications: natural brakes for CRISPR-Cas technologies. Nat Methods 17:471–479 . https://doi.org/10.1038...

... school when she was hired full time as a Lab Technician. “I was so tired of being in school so I just... is not true at all. Racheal Komuhendo, a Lab Technician, dreamed of becoming a doctor. After taking a...circumstance. She was working as a research and technical assistant, when her life changed––she had a daughter...

...of our mouse modeling blog series, we covered techniques that can be used to introduce genetic modifications...conditional knockin or knockout mice. While these techniques are each useful for introducing one modification...) and using PCR with the appropriate primers to detect your targeting vector or gene of interest. 2. Breed...

... of us learned in biology is that the body’s architecture and plumbing are important. We started with ...oxygenates the body. However, in neuroscience, this architecture is frequently still an open question. The connections...powerful development in neural tracing. Using these technologies in combination with molecular tools (e.g., optogenetic...

...there were unusual patterns of DNA methylation detected in a human disease: cancer (Feinberg & Vogelstein...the original methylated strand is diluted to undetectable amounts: ergo, DNA demethylation! With optimization...repeat and scFv–TET1 catalytic domain fusions. Nat Biotechnol 34, 1060–1065. https://doi.org/10.1038/nbt.3658...

...very easy — our tech transfer team will communicate with your organization’s technology transfer office... ship the samples to Addgene and contact your technology transfer office about the MTA. Pro Tip! Download...

...There can be no doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field... obstacles for future CRISPR therapies CRISPR technology is rapidly evolving, and many of the challenges...taken advantage of what our current delivery technologies are suited for. For example, lipid nanoparticle...

...antibodies and found that across suppliers there was a detection bias of IgG2a>IgG2b>IgG1 (Manning 2012).    ... the anti-mouse secondary antibodies used for detection bind to non-target immunoglobulins naturally present.... Chapter 15 Postembedding Immunocytochemical Techniques for Light and Electron Microscopy. In Methods...

...described above, and not any particular cloning technique. Though rarer, you can find MoClo that relies ...assembled into many combinations. Even with current technologies, it can be extremely hard to predict how a single... if you want to learn more about this cloning technique! Many thanks to Rob Hurt for his help on this ...

...biological tasks, both of which are factors in the technical challenges they can present. Many researchers ...hail Marys that have helped me troubleshoot some technical obstacles when working with virus: 1. Viral vectors... quantification as measured with branched-DNA technology." Journal of clinical microbiology 37.6 (1999...

...predicted off-target sites, Cpf1 does not mediate detectable off-target cleavage. Most gRNAs directed low-...specificities of Cpf1 endonucleases in human cells. Nat Biotechnol. 2016 Jun 6. PubMed PMID: 27272384 Find plasmids...-Cas Cpf1 nucleases in human cells." Nature Biotechnology (2016). PubMed PMID: 27347757. Find plasmids...

... Figure 1 (modified from Schnutgen et al. Nat Biotech 2003): Basic FLEx switch will express BFP in the... Figure 2 (modified from Schnutgen et al. Nat Biotech 2003): In the presence of Cre, GFP expression is... able to distribute materials containing FLEx technology. Read the genOway press release for more information...

...degradosome, which contains enzymes that cut RNA not protected by secondary structure. It is thought that poly... tail is important for stability of the mRNA, protection from degradation, and is integral to the nuclear...Controls Gene Expression Learn about New Cloning Techniques Read all of our Plasmids 101 Posts Resources...

...plasmid, using the fragment exchange (FX) cloning technique which uses Type IIS restriction sites (Geertsma...protein of interest. The Seeger lab used the ELISA technique described in their protocol because it is less...protein is added and after a few washes, binding is detected using a colorimetric reaction. After the ELISA...

...understanding of the fast, deadline-driven pace of biotech. In other words, industry postdocs already have...Biogen, Amgen, New England Biolabs, Regeneron, and Genentech, among others, and the list is growing. Some post... a position open up. Other companies, such as Genentech, will not. The point is that each company has ...

...the AAV production process. Either SYBR green technology or a TaqMan primer/probe set can be used and ...sample for the DNAseI digest. Use good sterile technique when handling your AAV plasmid standard and AAV...Baiker, A. (2012). Universal real-time PCR for the detection and quantification of adeno-associated virus serotype...

... on and so forth. This also serves as a light technical control - an RQ value of 1.5 means you have either...chemistry (see image, left, of droplet immersion technique, courtesy of CAR15.) Each droplet contains a primer...Molecular Biology Reference Molecular Cloning Techniques References Bell AD, Usher CL, McCarroll SA. ...

...Advantages of the agarose cloning method The agarose technique should be useful to scientists who wish to rapidly... from a population of transfected clones. The technique is applicable to a wide variety of cell lines,...physiological properties. An advantage of this technique is the potentially large number of single clones...

...Biologically Inspired Engineering. CRISPR/Cas9 technology has revolutionized the fields of molecular biology...discovery through the creation of innovative technologies. To date his group has develop several popular...catalytically active Cas9 nuclease." Nature biotechnology (2015). PubMed PMID: 26436575. PubMed Central...

...Baiker, A. (2012). Universal real-time PCR for the detection and quantification of adeno-associated virus serotype...adeno-associated virus vectors with sensitivity sufficient to detect single infectious events. Human Gene Therapy, 15...addgene.org  AAV Titration by qPCR Using SYBR Green TechnologyAAV Titration by ddPCRAAV Purification by Iodixanol...