We narrowed to 618 results for: abo.2

...the world’s population, we account for an estimated 2% of global plastic generation. Less than 9% of plastics...attended the Go green! Innovative practices for laboratory waste symposium, sponsored by New England Biolabs...Biolabs and Labconscious. I was eager to learn about practices I could bring both to Addgene, and share...offsets the cost of regular waste services. Learn about how to start a glove recycling program at your institute...lab tips for your research Read another blog post about conservation in the lab Resources on Addgene.org...

...easily understood and consistent in design and layout. 2. Be concise in your writing  When writing a manuscript...successful writer. When I originally talked to my PI about becoming a better writer, I was unsure if I should... a manuscript and critiquing it, were not too laborious, but helped build my understanding of how to generate...and the writing workshop. SWPI's writing workshop About a month later I received another email notifying...manuscript, scientists have a tendency to be elaborate and over explain the material. The point of writing...

... stabilization of unknown bacteria. Figure 2: Christina Mork collecting samples at the hot spring...Addgene blog Learn about other Cas proteins Read all of our CRISPR blog posts Read about GeoCas9, another... able to eliminate the need for axenic (pure) laboratory cultures to study these microbes. From 16S rRNA...by flexibly deploying IgnaviCas9 without concern about its stability under harsh conditions. One example...

...PMID: 30356044. PubMed Central PMCID: PMC6200774.   2. Nielsen, Alec AK, and Christopher A. Voigt. "Deep... Addgene blog Read blog posts about synthetic biology Learn more about codon usage biases Resources ...scientists from MIT tapped into this data to learn about trends in synthetic biology and DNA synthesis. They...synthetic just by looking at its sequence. MIT collaboration for gene classification This inquiry started...

...remained active in up to 30% plasma. Figure 2: Human plasma longevity of SpCas9 vs GeoCas9. SpCas9..., see the table below and read on to learn more about GeoCas9.   SpCas9 GeoCas9 Size 1368 AA 1087...vivo. What aspect of GeoCas9 are you most excited about? Thermostability? Longevity in human plasma? Something... Additional Resources on the Addgene Blog Read about CRISPR Methods for Bacterial Genome Engineering   ...

...HDR) and how it’s used in CRISPR genome editing.   2. Plasmids 101: Cre-lox Cre recombinase can be used...eyes open for any new technologies that will bring about the next revolution in biological research. We’re...Plasmids 101: Control Plasmids Need help thinking about the types of controls you’ll need for your next ...Addgene Browse Our CRISPR Resources Learn More about Viral Vectors Browse the Repository  ...

...access this tool, set up an IDT account. Figure 2: Addgene's Analyze Sequence tool.   DNA sequence...particular tasks, but they tend to be scattered about the internet. To help you keep your virtual molecular...other applications Primer design tools Many of the above-listed plasmid mapping tools have built-in primer...

...affinity and specificity. Trends in Immunology, 29(2), 91–97. https://doi.org/10.1016/j.it.2007.11.004 ...phagocytosis.  Other functions We’re still learning about Fc effector functions and all that antibodies can...isotypes and Fc receptors that we don’t know much about yet. Who knows what kinds of functions they may ...

...developed fluorescent-protein based DA sensors (Figure 2). The dLight1 dopamine sensor In 2018, Lin Tian’s ...making, and motor control.  What do we understand about Dopamine? DA is produced by specific dopaminergic...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

...light sensing proteins. Furthermore, during the first 2 weeks of life, larvae already exhibit distinct behaviors... posts about viral vectors Subscribe to the Addgene blog Resources on Addgene.org Learn about our ready-to-use...

...majority of deposited materials to study SARS-CoV-2 would be fully open and available to industry requestors...Our work accelerates and enhances productive collaborations as well as provides visibility for grant-driven...research is accelerating essential research in laboratories all around the world." We thank the Chan Zuckerberg...

...make it easier to study these mutants.   Fig. 2: (A) Betacyanin is produced from tyrosine by the activity... is difficult for new members of the lab or collaborators unfamiliar with these mutant plants. Using RUBY...resistant disease. (RUBY-Red: You can’t beet it!)   About the Author  Andrew Willoughby is a graduate student...

...interferon gamma (IFNγ) - and eliminate cancer cells. 2. Equilibrium - In a process analogous to the development... 35. Academic Press, 2015. PubMed PMID: 25818339. 2. Curiel, Tyler J., et al. "Specific recruitment of...potential beneficiaries. Collaborative therapy For the most efficient outcomes, collaborative therapies must be... of the tolerance-producing antigens enunciated above. Finally, signals released from the microenvironment...are in their clinical trial phase. In all of the above therapeutics, techniques like genome engineering...are beginning to see the fruits of many years of labor with up and coming immunotherapy treatments. As ...clinic, physicians and researchers are learning much about how treatment regimens can be improved and how the...

...establishing the dynamic range of your FRET assay (Figure 2). These labeling methods are freely interchangeable...PMID: 20161833. PubMed Central PMCID: PMC2802762. 2. Dumas, Anaëlle, et al. "Designing logical codon reassignment–Expanding...environment? If these are pressing issues in your laboratory, then FRET experiments are a viable biophysical...to consider these cases separately when talking about the tools that are available. Let’s consider the...that, if you make an FP fusion, you are inserting about 20-25 kDa of protein mass into your protein and ...together The FRET donors and acceptors described above can be paired together to measure a wide range of...of your protein that can lead to novel insights about its biology and its behavior. See the table below...

...development and therapy. Nat Rev Drug Discov (2017) 16(2):89-100. DOI: 10.1038/nrd.2016.238 Additional resources...type of edits can be made in the contexts outlined above. Layers of control have also been added to the CRISPR...

...method relies on the fluorescence ratio between the 2 fluorophores, rather than on the fluorescence intensity...CRISPR library from Alec Kimmelman targets mouse metabolic genes with ~6 gRNAs per gene.  CRISPR-Act3.0, ...

... master's degree prepared them for their career.  2. Are you looking for career advancement and growth... Resources Resources on the Addgene blog Learn about career planning resources What Do I Do Now? Academic...

...that underlie a particular phenotype. Figure 2: Schematic of CRISPR knockout screening with a pooled...variations in the spike protein, teaching us more about viral evolution and antibody binding. Figure...

...available to the community. We now offer AAV9 for 2 more constructs from Christopher Harvey’s lab. These...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

...biology 14.6 (2004): 757-764. PubMed PMID: 15582400. 2. Tomkinson, Alan E., et al. "DNA ligases: structure... And what is ligase fidelity anyway? Let’s talk about it. DNA ligases are enzymes that seal breaks in ...to accelerate characterization of nucleic acid metabolic enzymes." Nucleic acids research 44.2 (2015): ...

... remember that a resume is typically no more than 2 pages long. Write your resume for the job posting ...patent application processes? How about facilitating a collaboration between research groups? Perhaps ... a good job summarizing what a resume should be about. With a CV, information is usually presented chronologically...developed through your research? Don't simply write about the research you did. Emphasize the skills. If you... structures, then you might know a thing or two about computer modeling. Make sure to focus on these skills...the job you are applying for, in which case talk about your research, as an understanding of the topic ...you some advantage over other candidates. Write about your transferable skills Some of the skills you ...

...biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools, and a better...collate some of the current “best” FPs, and I collaborated with him on a web-based interactive visualization...deal more information that one might like to store about FPs than is currently represented at fpvis (complete...thousands of scientists routinely using and thinking about FPs, it would be great to have a centralized, community-editable...resource for storage and dissemination of information about FPs. FPbase.org FPbase is a project written in ...comprehensive community resource for information about fluorescent proteins.  It was designed with the ... there is plenty of room for improvement and elaboration.  Current projects include incorporation of biosensors...

...peer-mentoring groups at Addgene have gone virtual!   2. Practice public speaking For almost any science career...mentoring group (see above). However, now that we are all so good with video calls, how about trying your hand...blog or podcast, initiated and maintained a collaborative project, served on the board of a professional...appointments with people all over the world. Think about contacting alumni of your lab or university or use...suggest you speak with. See this post that’s all about informational interviews, for more details on how...progress on both building relationships and thinking about future career options. 5. Write a grant - even if...

...PMID: 30083608. PubMed Central PMCID: PMC6070312. 2. Stark, Jessica C., et al. "BioBits™ Bright: A fluorescent...student at MIT. While I had always enjoyed learning about biology in high school, it wasn’t until I started...college that I really fell in love with it. Something about being able to actually hold all those seemingly ...seemingly abstract biological reactions that I learned about from textbooks just made everything click in my head...students build circuits in physics class to learn about electronics or mix chemicals together in chemistry...a power source, but now you don’t have to worry about operating the rest of the car as well. Similarly...with us! We’ll work with you to get the word out about your plasmids and their potential educational uses...

...from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences in input concentration across ...”) of DNA, have changed our collective thinking about the feasible size and scope of projects in many ...next generation (NGS) sequencing have prevented laboratories from using these methods for routine processes...daunting for large numbers of samples. As mentioned above, one of the primary technical benefits of plexWell...1ng – 100ng) are normalized to two-fold variation above and below mean read count per sample, and the amount...NGS readout also reveals additional information about the presence of non-plasmid DNA or heterogeneous...   Check out the SeqWell website to learn more about our plexWell kits and services! Many thanks to Joe...

...created for the Brunello library is shown in Figure 2. You can use a small PCR product because the plasmids... and we thought it would be a good time to talk about the amplification and verification processes we ... DNA. These reactions should allow us to obtain about 100 to 1,000 bacterial colonies per individual plasmid...from the count_spacers.py file from Feng Zhang’s laboratory. There are a number of other analysis scripts...you have any tips, input, or stories of interest about library use, let us know in the comments! Additional...NGS Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries Resources on Addgene.org...

...automated. Unlike gel electrophoresis, you only need 1-2 ul of sample and the run time is just a few minutes...distinguish between DNA and RNA. See our protocol about using UV absorbance to quantify DNA Fluorescence...absorbance based methods, this method doesn’t tell you about contaminating proteins or chaotropic salts. Capillary... instruments are available. Some last reminders about quantifying DNA In choosing your DNA quantification...Additional resources on the Addgene blog Read more about molecular biology protocols and tips Find the best...best way to elute and store your plasmid DNA Learn about the different grades of plasmid DNA preps Resources...

... and whether I would be able to carry out a yeast-2-hybrid library screen; instead I thought they would...for Dummies”. The fastest way to learn something about marketing would be to start reading a broad simple...introduction to the financial world. It teaches you about selling products which is an important part of the...Seán on twitter @Macfhearraigh.    Read more posts about Scientific Career Development ...

... ligands can elicit varying degrees of β-arrestin-2 recruitment to receptors, indicating their agonistic...small-molecule fluorescent labels. Nature Methods, 19(2), 149–158. https://doi.org/10.1038/s41592-021-01338... A., Purkayastha, N., Flad, K., Schmidt, M. F., Tabor, A., Gmeiner, P., Hübner, H., & Weikert, D. (2020...

...is the most charged, with 5 negatively charged and 2 positively charged amino acids. FLAG tag has been ...Tags have a lot of major benefits – as discussed above – but there are a few instances when they should... hindrances, and destabilization can be brought about by the tag so it’s important to validate tagged ...

...our SPARC blog post for more details. Figure 2: Dense (c), intermediate (d), and sparse (e) labeling... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...non-overlapping cells. Until now, tools for doing this were labor-intensive and required using manipulations such ...

...containing complementary polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire B, Cogné B, Dupont...share the procedures and Python scripts with laboratories and viral vector cores routinely producing AAV...on Addgene.org Find AAVs at Addgene Learn more about Addgene's viral service Find protocols for working...

...Bacterial Cell Envelope. Cold Spring Harb Perspect Biol. 2(5) a000414 (2010). Cleveland Clinic. Gram Stain. https... it can be used in a lab? Here we’ll be talking about the ins and outs of this classification system, ...The classification system is also useful in a laboratory setting. When designing a plasmid to be inserted...

...worn outside of the BSL-2 area. BSL-2 laboratories must be clearly marked as “BSL-2.” The names and contact...addition to BSL-2 guidelines below, including PPE protocols . Working in a BSL-2 laboratory requires laboratory... biosafety level 1 (BSL-1) and biosafety level 2 (BSL-2). The purpose of the four levels is to distinguish...hygiene officer. BSL-2 Guidelines Remember, the BSL-1 laboratory guidelines above are expected to be followed...information of the laboratory manager should be clearly visible in the room and on the door. BSL-2 centrifugation... BSL-2 work receives a hepatitis B vaccination or titer prior to starting work in the laboratory. For ... steps to ensure you are working in BSL-1 and BSL-2 labs safely. Protocols...

...delivering an AAV construct expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused...I often wonder about the gut-brain axis (admittedly for self-serving reasons – I want to understand my...

...extraction protocol for more details.   Figure 2: An uncut plasmid is expected to form 3 bands: nicked...molecular biology and viral vector protocols Learn about the common lab E. coli strains Find tips for viral...

...structure-based framework engineering. Methods, 34(2), 184–199. PubMed PMID: 15312672. Inbar, D., Hochman...Additional resources on the Addgene blog Learn about visualizing protein turnover Download the fluorescent...

...yourself greater chances for success.  Build a team of 2 or 3 Don’t go it alone.  You’ll find that no matter...spring of 2015 and since that time have received about 3 project proposals per month.  We are currently...

...aspects of the work you presented may also be unclear. 2. Make the audience your primary focus When writing...  If someone asks a question about something they did not understand about your presentation, question ...important and relevant to them.   When you think about it, virtually every milestone in a scientist's career...scientists learn from advisers and other mentors by collaborating as they craft sentences for abstracts and presentation...  If someone suggests a change to a slide, think about whether the same principle applies to other slides...much bigger job than merely telling your audience about the work you've done. If you want to maximize the...expert audiences in other fields, like when collaborating with colleagues in other fields or writing grant...

...to inspire and open a new conversation about GMOs.  Challenge #2: Technical obstacles to plant synthetic...Revolution Bioengineering. Read their previous blog post about how they started their company here. The first transgenic... foundation of life on earth, and opportunities abound to build better fuels, feeds, foods, and fibers...not understand, and the complicated and unique metabolic processes of plants are being investigated on ...biochemical pathways involved in central anthocyanin metabolism are well understood. Additional work has also...knockouts to better understand developmental, metabolic, and photosynthetic processes. Often the tools...the color-changing flower, and we’d love to hear about the obstacles you’ve faced as a plant scientist,...