Addgene

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Advice for Moving Into Sales After Your Science Postdoc

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Blog Post

...especially for life science researchers. Many biotechnology companies are looking for PhD or Master’s level...
Hot Plasmids: FRET-Based Biosensors

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Blog Post

...tool for application in both neuroscience and immunology. Don’t be shy: Ratiometric imaging for beginners...
Extracurricular Activities for a Strong Science Career Path

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...Management Leadership Networking University technology transfer office positions Market analyses...
Visualizing Translation at the Single Molecule Level

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Blog Post

...Protein Plasmids Check Out Other Hot Plasmid Technologies ...
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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Blog Post

...which is buffered and contains the pH indicator phenol red. We froze samples in a -80°C freezer and then...
Addgene Depositors Get More Citations

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Blog Post

...plasmids: Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry & Molecular...
Four Factors that Differentiate the Stem Cell Field

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Blog Post

... stark contrast to recent genome engineering technologies, stem cell techniques are neither fast nor inexpensive...
The MTA at Addgene

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Blog Post

...approach, which we’ve updated and refined as new technology becomes available. Our one-time deposit agreement...
"Build Your Plasmid": The Game - Play to Teach

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Blog Post

...particularly interested in advanced in vitro models technology. Follow him on twitter @FRE_SCI. Additional...
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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...you stay connected. If possible, test out the technology before the conference begins.“There were some...
Revamp Your Lab Meetings With Creative Virtual Collaboration

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Blog Post

...something different from time to time breaks up the monologue a little and gives participants something else...
Visualizing Genomic Loci with CRISPR-Sirius

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...engineered sgRNAs using CRISPRainbow.” Nature Biotechnology 34 (2016): 528-530. PubMed PMID: 27088723 PubMed...
Improving Transduction Efficiency with Exosome AAVs

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Blog Post

...the classical purification protocol involving iodixanol gradients. The current purification protocols ...
New and Upcoming Viral Vectors - September 2019

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Blog Post

...of calcium dynamics. (For more details on this technology, see our blog post Overview of AAV Encoded Calcium...
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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Blog Post

...genetically encoded photosensitizer. Nature Biotechnology,24(1), 95-99. doi:10.1038/nbt1175. PubMed PMID...
R Bodies: Membrane-Rupturing Microscopic Tools

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Blog Post

...the Addgene Blog Read About Other Hot Plasmid Technologies Read Other Synthetic Biology Posts Suggest ...
Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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...CRISPR-Cas9 using asymmetric donor DNA." Nature biotechnology (2016). PubMed PMID: 26789497. 2. Lin, Steven...
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

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Blog Post

...between your bands. Stopping the gel when the bromophenol blue dye line is approximately 75-80% of the ...
Finding and Joining Your Dream Lab

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Blog Post

... and open source software, hardware, wetware, technology development, etc). Second, the potential hire...
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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Blog Post

...patient’s tumor sample, use cutting edge genomic technologies to map the mutations that are present, and use...
Addgene’s Viral Service - Why Virus? Why Now?

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Blog Post

...Without these scientists and the support of their technology transfer offices, Addgene would not be able to...
A Deep Dive into BioBuilder

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Blog Post

...inspire them and also keep up with all the new biotechnology.” These are the types of inquiries that routinely...
Viral Vectors 101: Viral Applications

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Blog Post

...over a hundred years ago. Since then, medical technology has changed a lot, but viruses have stuck around...
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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...editing, regulating and targeting genomes. Nature Biotechnology 32:347–355 . https://doi.org/10.1038/nbt.2842...
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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Blog Post

...Last updated Jul 20, 2021. By Will Arnold and Shreya Vedantam Accelerating research by improving the ...
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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Blog Post

...Cas domain compatibility and activity. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0453-z Additional...
Antibodies 101: ChIP

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Blog Post

... your DNA with a PCR purification kit or with a phenol-chloroform extraction. Fig. 1: Schematic...
Antibodies 101: Monoclonal Antibodies

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Blog Post

... of Murine Monoclonal Antibodies by Hybridoma Technology. JoVE. https://doi.org/10.3791/54832 Lipman ...
7 Tips to Secure a STEAM Internship This Summer

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Blog Post

...Several days prior to the interview, inspect your technology, background, and lighting for a smooth performance...
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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...interest. CRISPR-Cas9 tools have been used in immunology for in vitro screens of immune cell activity....
Targeted Mutagenesis with EvolvR

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Blog Post

...EvolvR-ing to targeted mutagenesis." Nature biotechnology 36.9 (2018): 819. Additional resources on the...
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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...a gBlocks® Gene Fragment from Integrated DNA technologies. Generally speaking, anyone using CRISPR/Cas9...
dTAG - You're it!

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Blog Post

...author on the recent paper describing the dTAG technology. Follow him on twitter @behnamnabet. References...
Visualizing Protein Turnover In Situ

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Blog Post

...Frankfurt am Main, Germany. She presented a novel technology, back then still unpublished, that allowed the...
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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Blog Post

..., has increasingly been recognized for its biotechnological applications including conversion of CO2 into...
The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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...study that influence tumor microenvironments: immunology and angiogenesis (Witz et al., 2009). Then, scientists...
Summer SciComm Series: Cognitive Load

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Blog Post

...leads us to… Framework Learning to think like an immunologist, a geneticist, a physicist, etc., is an immense...
Antibodies 101: Affinity Reagents

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Blog Post

...sensitive drug delivery systems. Elsevier Science & Technology Books. Bates A, Power CA. David vs. Goliath: ...
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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Blog Post

...expression at any copy number in bacteria." Nature biotechnology 36.4 (2018): 352. PubMed PMID: 29553576. 2. ...
A Look at Addgene's QC Process

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Blog Post

...dedicated scientists with expertise in sequencing technologies and bioinformatics. Through their efforts, the...

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