Plasmids 101: Origin of Replication
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pBluescript
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ColE1 (derivative) and F1**
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We narrowed to 784 results for: SCR;
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Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post...rRNA in bacterial RNA-Seq. This enriches for transcripts of interest without an increase in the number... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog Post...must be expressed from a plasmid or in vitro transcribed, and changes in gRNA secondary structure can ... -
Plasmid Cloning by PCR
TypeBlog Post...the downstream steps. For this example, we will describe how to copy a cDNA from one vector into a new ... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post... own experiences founding and running miLab to describe some key lessons I learned while becoming a health... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog Post...you particularly good at writing and editing manuscripts? Are you interested in scientific policy? Next... -
K. phaffii: Rising to the Occasion
TypeBlog Post...Organisms Plasmids 101: Yeast Vectors Tips for Screening with the Yeast Two Hybrid System Additional... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...s time, we’ve published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction... -
25 Work From Home Tips for Scientists
TypeBlog Post...visualizing your data, such as R or Python. Write a manuscript. If you have all the data you need to begin writing... -
Learning Technical Skills at a Lab Central Workshop
TypeBlog Post...of the workshop, NEB gave a brief presentation describing the technology. Then we split into pairs to actually... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog Post...shipment processes. After reading a publication describing how short term storage on dry ice can shift sample... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog Post...all over the world. The rest of this piece will describe plexWell™ in more detail, and how we are using... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog Post...site-saturation mutagenesis, and medium-throughput screening, she increased the affinity of the sensor for ... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...producer cells and purification protocols often describe a process that begins with cell lysis to release... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post...produce that green fluorescent protein through transcription and translation. Unfortunately, having live ... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post...cell cycle protein Emi1, showing that ~80% of transcripts with a long 5’ UTR were translationally silent... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog Post... LMB in Cambridge UK and then a postdoc at The Scripps Research Institute in La Jolla, California. She... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog Post...relatively modest, requiring expressed Cas9 protein, transcribed sgRNA, and optional donor DNA. Chris Richardson... -
How to Lead a Great Meeting
TypeBlog Post...points. At Addgene, we like to do this live on the screen using a Google Doc. Action items: Before leaving... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post...has a similar spectrum. For example, here's a screenshot from the Chroma Spectra Viewer comparing a standard... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post...time-intensive, so we recommend a variety of ways to screen and verify your plasmids. Here, we'll cover restriction... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post...relevant resources The homepage displays a graphic describing CasIDs and relevant properties of Cas enzymes... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog Post...region of an ITR with a sequence containing transcription factor binding sites can increase transgene ... -
Viral Vectors 101: Biosensors
TypeBlog Post.... Physiological factors Unlike the previously described sensors, this class measures either factors as... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog Post...through a seminal paper from Peter Schultz’s lab describing a technique that allowed for intentional incorporation... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post...Researchers around the world don't have to start from scratch every time to pursue their scientific curiosity... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog Post... — for example, the stem-loop structure of transcriptional terminators. Another potential issue is sequence... -
Hot Plasmids - May 2022
TypeBlog Post...expression of the fluorescent protein by a specific transcription response element (TRE) or kinase translocation... -
AAV Q&A with Tim Miles
TypeBlog Post...Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors Blog Posts ... -
Antibodies 101: ChIP
TypeBlog Post...your protein of interest is a low abundance transcription factor or cofactor. In such cases, the gentler... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog Post...consider the advice below: 1. Eligibility check Scrutinize every opportunity prior to starting an application... -
New and Upcoming Viral Vectors - May 2020
TypeBlog Post... genes and is used for genome-wide inhibition screening. Find the Dolcetto lentiviral prep here Control... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog Post...H2B-GFP. This visual read out allows for easy screening of different gRNAs by simply changing the gRNA... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog Post...the gene, the name of its gene product, and a description of its function. You can also click one location... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...the fluorescent protein properties need to be scrutinized under relevant conditions. The properties of ... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog Post...about Addgene in our "Inside Addgene" blog series Subscribe to the Addgene blog Resources on Addgene.org ... -
A Deep Dive into BioBuilder
TypeBlog Post...Murray’s lab uses cellular extracts to perform transcription and translation in vitro, and the new BioBuilder... -
Antibodies 101: Flow Cytometry Controls
TypeBlog Post...controls like FMO or isotype controls, which we’ll describe a bit later. Single Colors Single color controls... -
A Quick Guide to a Career in Software Product Management
TypeBlog Post...next-gen sequencing (NGS), and high-throughput screening. While some developers had science backgrounds... -
Plasmids 101: Yeast Vectors
TypeBlog Post...differ from the bacterial elements previously described. Why do scientists use yeast vectors? Yeast are... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post... this DNA structure can readily fold into transcriptionally active double-stranded form through intra-... -
Transferable Skills Guide: Public Speaking
TypeBlog Post...your slide deck gets uploaded. Your mic emits screeching feedback. You’re muted on Zoom. Your video conferencing... -
The time and cost required to make a plasmid
TypeBlog Post...plasmids, using both mass production genome wide screens and the more traditional “one at a time” approach... -
Antibodies 101: Fab Fragments
TypeBlog Post...talking about here? Many of the Fab fragments described above can be used for similar applications and... -
Antibodies 101: Selecting the Right Antibody
TypeBlog Post...-independent assays such as mRNA data from transcriptomics; independent antibody strategies that compare... -
Antibodies 101: Secondary Antibodies
TypeBlog Post...secondary will bind to all the primary antibodies indiscriminately, giving them the same output signal. In these... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog Post...RESOLUTE plasmid collection to perform a functional screen in mammalian cells to identify any solute channels... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post...Twitter @sejkeown. If you enjoyed this series, subscribe to the Career Channel of the blog so you don’t... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...Amsterdam. The previous two posts in this series described a practical approach to selecting a bright fluorescent... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...more efficient, as one does not have to thoroughly screen colonies for the insert. Gateway® technology (developed...