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  2. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...Damstra, et al. 2022; Yu, et al. 2020). With any variation, your samples can tear or expand unevenly, introducing...provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid...

  3. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...repair, with this rate varying across cell types. Variance can be caused by cell cycle rates; rapidly diving...mutated in cancer cells, specifically in breast and ovarian lines commonly used in lab. It is recommended you...

  5. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...infection and screening by PCR enrich for capsid variants with increased ability to infect the cell type... BP, et al. Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain...

  6. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...IPI’s recombinant version is a fusion of mouse variable and rabbit constant domains and is recommended...Tension Sensor (Zhong et al., 2024). In this new variation on their previous sensor STReTCh, the 11-amino...

  7. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...application. Even within an organization, there can be variance. At Addgene, our content team, where writing is... Good luck! This post was originally written by Maria Soriano-Carot in September 2016 and was updated ...

  8. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...target genomic locus/loci, choose a base editor variant from Table 1 depending on the PAM sequence. Avoid...Expanding the base editing scope in rice by using Cas9 variants." Plant biotechnology journal (2018). PubMed PMID...

  9. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...al., 2013, Cho et al., 2014). There are several variants of the CRISPR/Cas9 system floating around, and...and many pre-designed plasmids containing these variants ready for purchase. But what is the easiest and...

  10. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...however, that is generally not true. Because of the variations in fluorescent signals, and how the machine reads...SSC, as briefly alluded to above. There’s many variations in how one can gate, select, and analyze data...

  11. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...levels (Mauro and Chappell, 2014). Due to this variation in tRNA expression, the codons that encode a protein...and charged tRNAs to build a peptide. Image from Mariana Ruiz Villarreal. By the late 1980s, scientists...

  12. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange... mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly used with Clover pGWF1 Cyan & Yellow...

  14. TALEN Plasmids and Kits

    Type
    Collection
    ...GoldenGate-VP64 Golden Gate TALEN 2.0 47874 pTALYM3 Maria-Elena Torres-Padilla These plasmids were designed...

  19. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...systems, expression of S. pyogenes Cas9 or Cas9 variants (hereafter referred to as Cas9) and a single stranded... the nucleus, and several codon optimized Cas9 variants have been created in an effort to increase translation...

  20. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Tet-Off vectors (Tet-Advanced)” are the most recent variants of the Tet-Off system.  For more information on...pADH1 activity. Researchers have engineered pADH1 variants that are less sensitive to ethanol, including ...
Showing: 201 - 220 of 383 results