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  1. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...repair, with this rate varying across cell types. Variance can be caused by cell cycle rates; rapidly diving...mutated in cancer cells, specifically in breast and ovarian lines commonly used in lab. It is recommended you...

  2. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...IPI’s recombinant version is a fusion of mouse variable and rabbit constant domains and is recommended...Tension Sensor (Zhong et al., 2024). In this new variation on their previous sensor STReTCh, the 11-amino...

  3. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...application. Even within an organization, there can be variance. At Addgene, our content team, where writing is... Good luck! This post was originally written by Maria Soriano-Carot in September 2016 and was updated ...

  4. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...however, that is generally not true. Because of the variations in fluorescent signals, and how the machine reads...SSC, as briefly alluded to above. There’s many variations in how one can gate, select, and analyze data...

  5. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...target genomic locus/loci, choose a base editor variant from Table 1 depending on the PAM sequence. Avoid...Expanding the base editing scope in rice by using Cas9 variants." Plant biotechnology journal (2018). PubMed PMID...

  6. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...levels (Mauro and Chappell, 2014). Due to this variation in tRNA expression, the codons that encode a protein...and charged tRNAs to build a peptide. Image from Mariana Ruiz Villarreal. By the late 1980s, scientists...

  7. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...al., 2013, Cho et al., 2014). There are several variants of the CRISPR/Cas9 system floating around, and...and many pre-designed plasmids containing these variants ready for purchase. But what is the easiest and...

  8. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...Clover Green Mammalian Expresses Clover (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange... mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly used with Clover pGWF1 Cyan & Yellow...

  10. Plant Plasmids and Resources

    Type
    Collection
    ...selection of plasmids expressing different functional variants of Cas9 and empty gRNA backbones. A few examples...

  11. p53 Pathway

    Type
    Collection
    ...: 24739573 . Uncovering the role of p53 splice variants in human malignancy: a clinical perspective. Surget...

  12. TALEN Guide

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    Collection
    ...from each other by two amino acids, their repeat-variable di-residue (RVD). Ultimately, it is this RVD that...

  13. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...systems, expression of S. pyogenes Cas9 or Cas9 variants (hereafter referred to as Cas9) and a single stranded... the nucleus, and several codon optimized Cas9 variants have been created in an effort to increase translation...

  18. Control AAV Preps

    Type
    Collection
    ...Promoter CAG CaMKIIa CMV Dlx EF1a/nEF GFAP and variants Synapsin TBG Other Fluorophore/Tag Green Red None...

  19. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Tet-Off vectors (Tet-Advanced)” are the most recent variants of the Tet-Off system.  For more information on...pADH1 activity. Researchers have engineered pADH1 variants that are less sensitive to ethanol, including ...

  20. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...to the intended edit rather than some passenger variant that came along with the single cell clone (although...SaCas9, NmeCas9, Cas12a enzymes, and engineered variants thereof offers additional PAM options that can...
Showing: 201 - 220 of 383 results