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We narrowed to 970 results for: Abo;

Showing: 201 - 220 of 970 results

  1. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ...Northeastern University, she interviewed Addgenies about their career path. Graduate school: Do I or ...or don’t I? I asked the nine Addgenies about why they did or did not go to grad school. QC Scientist Alyssa...our Financial Operations Specialist, Lucy Hammer, about why she did not go. She told me, “I think that my...Perkins noted that there is a more emphasis today about non-academia and industry jobs. Since there are ...to keep my values in mind as I applied. Thinking about this made me curious. I wondered what my coworkers...view of science. It should be really open and collaborative.” She knew she wanted to leave academia, and...from my co-op. It has been so insightful to learn about different career paths, successes and failures, ...

  2. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...School visited our labs in Cambridge, MA to learn about the wide variety of science careers that Addgenies...s never too early to start networking! Learning about careers at Addgene Afterwards, the students split... was very cool!” and Izzy Shaw, who was excited about “Seeing a real lab!” And several of our visitors...visitors made sure to tell us what we already knew about our Shipping Operations Coordinator: “Kory was awesome...awesome!”. The students also got to learn about intellectual property from our Tech Transfer team members...are offered with a biology major.” After hearing about many Addgenies’ career paths, Michelle Healy said...work to help improve K-12 education? Let us know about your educational events in the comments section ...

  3. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...carrying a synthetic gene drive were to escape the laboratory, the drive could eventually spread through the...mistake would necessarily extend far beyond the laboratory and seriously damage public trust in scientists... can spread through populations of transgenic laboratory organisms but not wild organisms. For example...synthetic sequence will only spread in transgenic laboratory populations with that sequence2. Both methods...Reproductive confinement involves working with laboratory organisms that can't reproduce with wild ones...confinement seeks to keep the organisms in the laboratory. It varies by organism, but your local biosafety...population and immunizes it against the first drive. Laboratories interested in building candidate gene drives...

  4. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...When you think about going to a scientific conference, you may think about sitting amongst a sea of chairs...After learning about using sound to visualize bacteria in the lab, I was excited to learn about creative approaches... at different ultrasound frequencies. Read more about ‘Using ultrasound to image bacteria in vivo: acoustic...Protein Resources and get started!  I learned a lot about creative ways to do science communication and outreach...outreach - if you'd like to learn more about Science communication check out these SciComm blog posts.... Methods for Bacterial Genome Engineering Learn about Science Communication using Twitter  Resources ...

  5. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    ...scientists to make genomic alterations, bringing about a revolution in genome engineering, with new techniques...available by both the Jacob Corn and Alex Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins  An...delivery of RNPs is electroporation (A in the figure above), which generates pores in the cell membrane, allowing...as lipid-mediated transfection (B in the figure above), are in the early stages of development. David ...proteins harboring receptor ligands (C in the figure above), which result in the internalization of Cas9-gRNA...show promise for in vivo studies, little is known about the immunogenicity and stability of the Cas9 protein...and purification, which will require additional laboratory reagents, equipment, and expertise. Final thoughts...

  6. How to Write a Scientific Review Article

    Type
    Blog Post
    ...didn’t have to worry about having to hunt down sources later on.  A comment about writing: initially I...exists. Once you pick a topic, try to be specific about exactly what aspect of the field you plan to review...themes in the field and to learn what people cared about. I also used reviews to get a list of research papers...highlighted a few dozen things that I didn’t know about. Instead of reading a paper and getting my bearings...them. No really, just start writing! Don’t worry about grammar or formatting or continuity. Also don’t ...worry if you feel like you don’t still know enough about the topic. Just get as many words down on the page...between office hours and experiments. A comment about citing: I wasn’t sure if I should cite reviews or...

  7. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ...The Addgene-Seeding Labs plasmid grant is a collaborative endeavor providing plasmids to researchers in... of Zimbabwe, Zimbabwe Dr. Idah Sithole-Niang (above, left) is an Full Professor in Biochemistry with... CRISPR effect observed in bacteria. Learn more about the different CRISPR applications in plants.     ...Rodrigo Arturo Rivera Solís, Associate Professor (above, far right), thrives to make affordable biosensors...detect glyphosate via H2O2, one of the reaction’s metabolites. One useful genetically encoded H2O2 probe is... to the Addgene blog for updates and future collaborations. Many thanks to all of the awardees, all who...Additional Resources on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out...

  8. Choosing a Good Mentor for Scientists

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    Blog Post
    ... will also serve as a good mentor. To read more about what makes a good mentor, see the previous post ... and personnel management?  If you are thinking about a non-academic path, will the PI support this path...Medical School researcher Connie Cepko to learn about her mentoring style. How can I find out if an advisor...time with the potential advisor and don’t be shy about asking hard questions. Talk to as many lab members... past lab members. Most scientists love to talk about their past lab experiences and will be willing to...members, neighbors or alumni give you information about the PI that concerns you, don’t just brush this ...Mentors by @drmellivora, an excellent blog post about workplace bullying featured on the always interesting...

  9. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...Cell tracing in zebrafish with Zebrabow Before the generation of Zebrabow (Pan et al., 2011), Brainbow ...crossing of Zebrabow animals to an inducible Cre line. The Schier group developed Zebrabow as a system...by injecting Brainbow at the single-cell stage. Zebrabow, or “zebrafish brainbow,” is a collection of transgenic...are introduced using a plasmid construct called Zebrabow-GateDest containing an RFP, CFP, and YFP downstream...downstream of a ubiquitous promoter. Zebrabow is an extremely versatile technology as recombination can be...an introduction to live cell imaging Learn more about the origin of the Brainbow system Visit our fluorescent... Addgene’s fluorescent protein collection Learn about fluorescent tools for in vivo imaging ...

  10. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...a successful negotiation and collaboration. We’d love to hear more about your experiences and opinions...can get away with using computer modeling and collaborations rather than using animals, then that’s a cost...associated with old equipment, like service repairs, labour costs, and loss of time, can exceed the cost of...Everything is negotiable. Try asking for discounts, collaborations, access to internal award applications, etc...This is a non-profit organization. Therefore, collaboration and community is the ultimate value proposition...Happy Sciencing, Damien   Damien Wilpitz is a laboratory research management consultant and coach in Boston...research scientists create productive and manageable laboratory environments. His business skills enhance his...

  11. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...the Addgene Blog Learn about Efforts to Fight Rare disease with iPSCs Read About Addgene's Outreach Efforts...the first clinical trial with iPSCs with his collaborator Takahashi in 2014 – just 8 years after the OSKM...researchers special: 1. Stem cell scientists care deeply about ethics Ethics must play a prominent role in the ...Researcher Darrell Kotton 2. Stem cell scientists care about patients As any grad student who has spent years... a big picture perspective: Dr. Yamanaka talked about his clinical trial patients with advanced macular...proof that these researchers are thriving in a collaborative, rather than a combative, field. Of course they... in the Microbiology Community Sign Up to Write About Stem Cells Resources on the Addgene Wesbite Find...

  12. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...fully-equipped laboratory for citizen scientists. In addition to maintaining a laboratory to support both... of independent Do-it-Yourself (DIY) biology laboratories working towards enabling everyone to participate...Anyone can become a member of BosLab and previous laboratory experience is not required. Our members are skilled...have non-traditional scientists working in our laboratory! Don’t worry though, we make sure that everyone...samples. The goal is to not only learn something about the microbial diversity in different soil conditions...community lab in a city near you. To learn more about what we’re up to or how you can help BosLab, visit...a co-organizer at BosLab, a community biotech laboratory in Somerville MA. Follow her on twitter at @ZebraElement...

  13. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... communication, and in particular communication about experimental methods and approaches. It’s very easy...everything will be fine. It won’t. When we hear about issues with reproducibility, time and time again...the problem comes down to inadequate information about what the original researchers actually did. Admittedly...Communication comes in many forms—from transparency about data to allow others to see what you actually found...fits together to make for a transparent and collaborative research environment. Journals as enforcers ...of methods sections, including clear information about reagents, and why other publishers and organizations... in reproducibility jail and more effective collaboration to drive the progress of science. Sounds like...

  14. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...communication in remote collaborations Transferable Skills Guide: External collaborations Transferable Skills... stint may be ideal for you. Here, we will chat about who might want to explore this role, how to seek...scientist is distinct from equipment sharing or a collaboration – it requires re-location of the scientist to...reasons Visiting gives you access to possible new collaborations and fresh perspectives. For trainee level researchers...future work placements. The chance to learn and collaborate all while experiencing a new city or maybe even...there is someone you personally know or your lab collaborates with that can fulfill your research needs, that... open to sharing knowledge and establishing collaborations, so it doesn’t hurt to reach out and ask if...

  15. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...see the new map in action). Read on to learn more about the improvements we’ve implemented and how they’...more functional display, you can start thinking about your next cloning experiment early on. Beyond these... to to download the static image file (PNG, see above) for your own reference or to paste directly into...identified a plasmid that you want to learn more about, updates to our sequence analyzer will allow you...the features shown in the static maps discussed above, but also have interactive functionalities. You ...enzyme, primer, or ORF to learn more information about it. For example, if you hover over the ampicillin...guide A quick guide to all of the tabs listed above and their functions. We hope that these updates...

  16. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...designed (listed above) worked well and I got clear bands using the conditions above. 2. Design your primers...essential for experiments but that nobody talks about. After months of troubleshooting, those people who...details. The entire process from start to finish took about 1 month. Cas9 delivery Overview: Before any ...is a loading control. Pro-Tips: 1. It’s all about the MOI. In retrospect I used a way too high Cas9...original publication they see GFP expression in about 20% of their Cas9-expressing cells. So, even when... gene has a weird mutation that you didn’t know about (which could affect the ability of the gRNA to bind...engineering has come. For comparison, in 2007 it took about 3 months to knockout a single allele in a human ...

  17. 6 Tips for Grant Writing

    Type
    Blog Post
    ...introduction to my area, a line about the research I will be doing, and a line about what I hope to discover....themselves many times and will have useful advice about what reviewers in your area are looking for. Remember...One way to add originality to your work is to collaborate with specialists in different fields that can...the field. 3. Highlight your network and your collaborators It’s a good idea to move around to different...outcomes can include poster presentations, talks about your work, or even the successful use of your preliminary...quality application. Even following this advice, only about 50% of my grant applications have been successful...on your grant application to your peers and collaborators can also help you get some critical insight ...

  18. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...Addgene Blog Learn about Synthetic Biology with BioBuilder Use Addgene Videos to Learn about Basic Lab Techniques...species, but these studies generally leave questions about gene function wide open. What if two seemingly similar...been using yeast models (3, 4) to answer questions about the evolution of the genes involved in methionine...question, the plasmids are also used to teach students about restriction mapping and western blotting. Students...communicating science to the public. You can find out more about Clare at the Biology Dept. site.  References 1. ...PMC4596657. 5. Thomas, D., & Surdin-Kerjan, Y. Metabolism of sulfur amino acids in Saccharomyces cerevisiae...Functional genomics research in an introductory laboratory course. CBE-Life Sci. Educ. 17, ar1. (2018). ...

  19. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...the Addgene Blog Learn More about Homology Directed Repair Learn More about Non-Homologous End Joining .... For a C-terminal tag, as shown in the example above, we inserted a linker and GFP tag at the end of ... and 1kb of homologous DNA flanking both sides (about 2.7kb total)—is inserted into a plasmid backbone... Plasmids, plasmids everywhere To find out more about gene edited hiPS cell lines generated using the ...line. The editing strategy and design described above can be used to create similar donor plasmids for...microhomology-mediated end-joining (MMEJ). To find out more about gene edited hiPS cell lines generated using these...

  20. Podcast: A Malawian Professor's Path to Biotech Research

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    Blog Post
    ...more about Dr. Kwapata’s current work and thoughts on the need for more scientific collaboration across... connecting us with him. Learn more about our recent collaboration with Seeding Labs here. Special thanks...received an award from nonprofit and Addgene collaborator, Seeding Labs, for lab equipment that will help...Addgene Podcast.     Additional Resources Learn about Seeding Lab's Work with Lilongwe Univeristy of Agriculture...How You Can Contribute to the Addgene Blog Learn about Addgene Plasmid Donations for Rare Disease Research...
Showing: 201 - 220 of 970 results