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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 712 results for: BASE

Showing: 201 - 220 of 712 results

  1. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...robust protocol it does have limitations. The enzyme-based CUT&RUN method overcomes many of these limitations...interacting. The CUT&RUN method Enter CUT&RUN, an enzyme-based protocol for identifying DNA binding patterns that...sets, of which there are many readily available. Based on the success that our lab and others have had ...

  2. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...from some labs have developed fluorescent-protein based DA sensors (Figure 2). The dLight1 dopamine sensor... dLight1 sensor is a single fluorescent protein-based sensor. This sensor was engineered from a GFP variant...sensor Yulong Li’s lab has created the GRABDA sensor based on a similar strategy: a GFP variant bound to a ...

  3. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    ... or other products in the suite. Since it’s web-based, you could access it from anywhere. Its ease of ...Biorender is like clip art for scientists. The web-based collection of over 20,000 icons is designed by professional...stop struggling to make figures in PPT 🔬 Also, the base version is free, forever 💜 https://t.co/KJpyxgxeQt...

  4. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ... level with CellTag CellTagging is a lentiviral based tagging method that labels cells with heritable ...three-fold increase of fully reprogrammed cells based on single-cell gene expression patterns. The lab...Chuner, et al. "CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics." bioRxiv...

  5. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...polymerase, the construction of specific sequences for base pair overhangs, insert and vector purification, ...specific to the insert with an additional 15-17 bases that are complementary to the overlapping vector...introduced into my inserts. However, with any PCR-based cloning technique there is a chance of introducing...

  6. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...tells us about a new tool for efficient multiplex base editing and multiplex prime editing (requiring minimal...dCas9 (with no modifications!) as a CRISPR/dCas9-based tool for DNA demethylation. AAV Q&A with Tim Miles...

  7. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation ...

  9. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...short ncRNAs, such as microRNAs that are around 20 base pairs long. Using a single guide RNA (sgRNA) to ...Figure 1: A schematic illustration of CRISPR/Cas9-based approaches to study ncRNAs. (A) Transcription start...your experiments, there are plenty of other CRISPR-based options that can target ncRNAs.  CRISPRi and CRISPRa...10.1126/science.1225829  Hazan J, Bester AC. CRISPR-Based Approaches for the High-Throughput Characterization...

  10. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...therapeutic potential. Note: constructs discussed are based on Adenovirus type 5. Three generations of adenoviral...delivery: Potential applications for gene and cell-based therapies in the new era of personalized medicine...formation assay on A549 cells . Alternatively, antibody-based assays are commercially available for faster detection...

  11. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...American, Africa, or South Asian) used in the screens based on the cells’ germline genetic variations. They ...generating genetic dependency data with an ancestry-based bias.   The correction process does not stop there...genome-wide screens, introducing bias into all work based on these and any other CRISPR screens using the ...variation contributes to false negatives in CRISPR-based experiments with varying burden across ancestries...Cancer Dependency Map (DepMap), a cancer dependency database used to make this information accessible to the...

  12. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...packaging systems have been developed over the years based on HIV-1; however, the 1st-generation vectors are...defines 2nd- or 3rd-generation transfer vectors based on whether or not they have a chimeric 5'LTR. Second-generation...? What percent of the vector components are HIV-based? A7: No single plasmid contains all the components...insertional mutagenesis. These risks are largely based upon the vector system used and the transgene insert...

  13. Fluorescent Protein Guide

    Type
    Collection
    ...backbones. Fluorescent Protein Kits cpFRET Kit: FRET based biosensors - Pertz Fluorescent Protein Fusion Construct...Resources FPbase from Talley Lambert at Harvard Medical School Fluorescent Biosensor Database designed ...Galleries from Michael Davidson and FSU Spectra Database hosted at the University of Arizona Zeiss Education...

  14. AAV Viral Preps

    Type
    Collection
    ...CRISPR Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing Samples Small samples ...

  15. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...Since the number of base pairs for each varies, it is difficult to calculate this based on DNA concentration...restriction sites to flank your insert : You can use PCR Based Cloning and add restriction sites to the ends of...

  17. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    ...and jargon Hemingway Editor  This is a free web-based tool for detecting the readability of your writing...or dissertations. Phrases are categorized either based on the section of a manuscript they might be found...to work with collaborators. Zotero is a desktop based program that’s compatible with Mac, Windows, and...directly import papers from web pages and academic databases.  Downloading Mendeley Cite lets you cite papers...

  18. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...plexWell™ Library Preparation Technology for NGS-based sequencing and confirmation of Addgene’s large and...plexWell™ workflow also offers a highly efficient NGS-based alternative to Sanger sequencing because the sample... To date, this has resulted in high quality NGS-based assemblies for over 3000 plasmids in the Addgene...

  19. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...While there are more and more molecular biology-based activities making their way into high school classrooms...BioBits ExplorerTM and BioBits BrightTM, biology kits based on freeze-dried, cell-free (FD-CF) components. Making...and is interested in developing synthetic biology based tools for science outreach and advocacy. You can...

  20. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...This version of the spike protein was developed based on 100 structure-guided spike designs and has improved...already developed nucleic acid detections tools based on Cas12a and Cas13  which could be repurposed for...JA, Finkelstein IJ, McLellan JS (2020) Structure-based design of prefusion-stabilized SARS-CoV-2 spikes... CM (2020) Open Sharing During COVID-19: CRISPR-Based Detection Tools. The CRISPR Journal 3:142–145. https...
Showing: 201 - 220 of 712 results