...sensitivities can now be applied to dozens of other published FRET-based sensors. For example, his team is now... improve upon an indicator they developed and published in 2010 for visualizing the transcription factor...

...control). The end result of the meeting will be a publicly shared practical guide for all scientists in the...prepare and compile resources for this meeting's publication, we are asking participants to share practical...have final approval before anything is released publicly, but please only share materials that you are ...materials that you have permission to share (e.g., publication's permission). Connie Cepko, Harvard Medical School...section for how to best help contribute to this publication. Citation information for openly shared communication...

...PMCID: PMC6070313. Find the plasmids from this publication at Addgene! Additional Resources on the Addgene...pages Find plasmids from the BioBits BrightTM publication here ...

...Do-it-Yourself (DIY) biology laboratories working towards enabling everyone to participate in the biotech revolution...detect compounds in the environment, and openly publish protocols so that anyone can engineer yeast to ...

...identified MerMAIDS from their analysis of different publicly available metagenomic data including the Tara ...demonstrates the power of combining the analysis of publicly available metagenomic datasets with biophysical...

...vectors VSV-G and psPAX2, which were used in a publication by Xiuyuan Ou, et al. that characterized the ... requested these plasmids from Addgene. This publication then resulted in the development of a new plasmid...

...do just this.  In under a month’s time, we’ve published an article on BioRxiv describing a comprehensive...coronavirus, one FedEx package at a time Since the publication of our paper, our group has been inundated with...

...this screen successfully differentiated cells resembling all three germ layers (ectoderm, endoderm, and...amplification, refer to the Zhang lab’s previously published protocol and its relevant modifications on the...

...researchers are open to sharing knowledge and establishing collaborations, so it doesn’t hurt to reach ...expect to generate data that will contribute to a publication? A grant? A patent? It is never too early to ...

...libraries are popular tools for genetic screens, enabling genome-wide interrogation of the genes required...are refractory to lentiviral transduction, now enabling forward genetic screens in cells with few genetic...

...Davidson and Roger Tsien played enormous roles in enabling researchers to utilize and develop these tools...Update (7/17/2018): Check out Fatima's recent publication on her HMO work in Cell Chemical Biology! Additional...

...nucleofection The in vivo editing experiments in this publication were performed using a technique called nucleofection...Additonal Corn Lab Members who worked on this publication:         References 1. Richardson, Christopher...

...available. With new fluorescent proteins being published every month, deciding on the best protein for ...common use (particularly not the most recently published ones), they can be a good starting point. In many...

...In the blink of an eye, the long days in the lab as a graduate student and postdoc come to an end and...who were “chalk-and-cheese”. While one was an established Professor who was always “off flying his plane...

...CRISPR Plasmids Zinc Finger Consortium Originally published in Addgene's December 2011 Newsletter Overview...Halle-Wittenberg and Adam Bogdanove at Iowa State University published the nucleotide recognition code of the TAL effectors...can be made. Intuitively, one would expect that assembling relatively small, repeat-laden DNA regions into... before the end of the year. Dr. Zhang ‘s lab published a paper on TAL effectors in Nature Biotechnology...early in 2011 and they described their kit for assembling TAL effector arrays in a 2012 issue of Nature...

...lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates Addgene’s founding...plasmid was called pLKO-Tet-On in the original publication but its name was subsequently changed to clarify...

...resulting in a fluorescent signal. Since their first publication in 2012, the Nagai laboratory has assembled a...sensitivity and faster imaging compared to previous publications. They also developed calcium, cAMP, and ATP ...

...to make the sequences of monoclonal antibodies publicly available. The Neuro Mab Sequencing Initiative...Trimmer lab hybridoma collection and make them publicly available. The lab has also expanded their recombinant...

...tissues. Although antibody production is well established, it’s also a process that’s difficult for individual...their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following...

...to find the simplest way to do it (i.e. avoid assembling too many fragments at once, if it is possible...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ...