We narrowed to 564 results for: BLI
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TypeBlog Post...available. With new fluorescent proteins being published every month, deciding on the best protein for ...common use (particularly not the most recently published ones), they can be a good starting point. In many...
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Advice for Scientists Starting a Lab
TypeBlog Post...In the blink of an eye, the long days in the lab as a graduate student and postdoc come to an end and...who were “chalk-and-cheese”. While one was an established Professor who was always “off flying his plane... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates Addgene’s founding...plasmid was called pLKO-Tet-On in the original publication but its name was subsequently changed to clarify... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...resulting in a fluorescent signal. Since their first publication in 2012, the Nagai laboratory has assembled a...sensitivity and faster imaging compared to previous publications. They also developed calcium, cAMP, and ATP ... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog Post...to make the sequences of monoclonal antibodies publicly available. The Neuro Mab Sequencing Initiative...Trimmer lab hybridoma collection and make them publicly available. The lab has also expanded their recombinant... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog Post...to find the simplest way to do it (i.e. avoid assembling too many fragments at once, if it is possible...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ... -
RANbodies: Reporter Nanobody Fusions
TypeBlog Post...tissues. Although antibody production is well established, it’s also a process that’s difficult for individual...their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following... -
Antibodies 101: Conjugation
TypeBlog Post...BioRender.com. Many biologists can lead perfectly blissful, productive lives without thinking about these...10.1016/j.it.2012.02.010 Dennler P, Fischer E, Schibli R (2015) Antibody Conjugates: From Heterogeneous... -
Addgene: The First Twenty Years
TypeBlog Post...was frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan...others could request from — Addgene! Addgene was established as an e-commerce company, which helped it stay... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post... research environment” with a strong focus on publishing, but still made it so she could “get exposure...summarized in a recent Nature Medicine paper. In the publication, Bhang and coworkers used the ClonTracer library... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...they’ve gone along, Voigt’s group has also been assembling the toolbox needed for anyone to begin considering... a very big way. In one of his latest papers, published in Molecular Systems Biology in November, Voigt... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology... E, Bondy-Denomy J, Corn JE, Doudna JA (2017) Disabling Cas9 by an anti-CRISPR DNA mimic. Sci Adv 3:e1701620... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...name and match the name as it appears in its publication or as it is commonly known in your lab. Plasmid...Distribution status for your plasmids: Hold for Publication or Distribute pending QC. The name of the Principal... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...bacteria can also have serious implications for public health. Bacteriocin plasmids: microbial offense...toxic substances like heavy metals or pesticides, enabling their hosts to survive where others can’t. This... -
Making CRISPR Plasmids Using Fragmid
TypeBlog Post...the Fragmid system by sequencing 60 individual assemblies created over five months. When they tested those...were perfectly correct. Because they created the assemblies in duplicate, they were able to confirm that ... -
Plasmids 101: Modular Cloning
TypeBlog Post..., a pre-made MoClo kit, is a prime choice for assembling an array of targeting constructs and delivering... many different MoClo kits have already been established. Keep an eye out for our next blog on choosing... -
Hot Plasmids February 2024
TypeBlog Post...original, though its maturation is even faster. Published just a few days later, Mohan Balasubramanian and...antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing. In a proof-of-concept study, mouse... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...Originally published Apr 30, 2020 and last updated Jul 12, 2021. What started out as a small collection...lab instead of a BSL-3 lab. Jesse Bloom’s lab published a protocol and reagents for pseudotyping lentivirus... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post...Cytochemistry (Vol. 46, Issue 8, pp. 977–983). SAGE Publications. https://doi.org/10.1177/002215549804600813 ...Sturtevant (Ed.), PLoS ONE (Vol. 7, Issue 6, p. e38313). Public Library of Science (PLoS). https://doi.org/10.1371... -
CRISPR in the Clinic
TypeBlog Post...substantial relief from both disorders. As of publication, submissions to the UK, EU, and USA federal drug...cardiovascular disease, inflammatory diseases, blindness, and diabetes. Directions and obstacles for future...