Skip to main content
Addgene
Showing: 201 - 220 of 562 results

  1. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...In the blink of an eye, the long days in the lab as a graduate student and postdoc come to an end and...who were “chalk-and-cheese”. While one was an established Professor who was always “off flying his plane...

  2. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...resulting in a fluorescent signal. Since their first publication in 2012, the Nagai laboratory has assembled a...sensitivity and faster imaging compared to previous publications. They also developed calcium, cAMP, and ATP ...

  3. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates Addgene’s founding...plasmid was called pLKO-Tet-On in the original publication but its name was subsequently changed to clarify...

  5. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...tissues. Although antibody production is well established, it’s also a process that’s difficult for individual...their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following...

  6. Addgene: The First Twenty Years

    Type
    Blog Post
    ...was frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan...others could request from — Addgene!  Addgene was established as an e-commerce company, which helped it stay...

  7. Antibodies 101: Conjugation

    Type
    Blog Post
    ...BioRender.com.     Many biologists can lead perfectly blissful, productive lives without thinking about these...10.1016/j.it.2012.02.010 Dennler P, Fischer E, Schibli R (2015) Antibody Conjugates: From Heterogeneous...

  8. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...to find the simplest way to do it (i.e. avoid assembling too many fragments at once, if it is possible...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ...

  9. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ... research environment” with a strong focus on publishing, but still made it so she could “get exposure...summarized in a recent Nature Medicine paper. In the publication, Bhang and coworkers used the ClonTracer library...

  11. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...name and match the name as it appears in its publication or as it is commonly known in your lab. Plasmid...Distribution status for your plasmids: Hold for Publication or Distribute pending QC. The name of the Principal...

  12. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...bacteria can also have serious implications for public health. Bacteriocin plasmids: microbial offense...toxic substances like heavy metals or pesticides, enabling their hosts to survive where others can’t. This...

  13. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...the Fragmid system by sequencing 60 individual assemblies created over five months. When they tested those...were perfectly correct. Because they created the assemblies in duplicate, they were able to confirm that ...

  14. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ..., a pre-made MoClo kit, is a prime choice for assembling an array of targeting constructs and delivering... many different MoClo kits have already been established. Keep an eye out for our next blog on choosing...

  15. Hot Plasmids February 2024

    Type
    Blog Post
    ...original, though its maturation is even faster. Published just a few days later, Mohan Balasubramanian and...antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing. In a proof-of-concept study, mouse...

  17. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...Cytochemistry (Vol. 46, Issue 8, pp. 977–983). SAGE Publications. https://doi.org/10.1177/002215549804600813 ...Sturtevant (Ed.), PLoS ONE (Vol. 7, Issue 6, p. e38313). Public Library of Science (PLoS). https://doi.org/10.1371...

  20. CRISPR in the Clinic

    Type
    Blog Post
    ...substantial relief from both disorders. As of publication, submissions to the UK, EU, and USA federal drug...cardiovascular disease, inflammatory diseases, blindness, and diabetes. Directions and obstacles for future...
Showing: 201 - 220 of 562 results