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We narrowed to 648 results for: CHI

Showing: 201 - 220 of 648 results

  1. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...   Many thanks to our guest blogger, Joachim Goedhart! Joachim Goedhart is an assistant professor at ...This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of ...fluorescent probes. You can follow him on twitter: @joachimgoedhart.   References 1. Zacharias, David A., et al...

  2. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ... are part of engineered chimeric antigen receptors (CARs). CARs are a chimera of scFvs and T cell receptors...anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proceedings of the National Academy of ...A, Sivaccumar JP, Ruvo M (2020) Evolution of Escherichia coli Expression System in Producing Antibody ...

  3. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...1: Indirect detection of target markers can be achieved through two-protein labeling by utilizing(a) biotin-streptavidin...e.g., for cellular activation. In addition, FACS achieves greater purity of your sorted cell sample than...proliferation between cell populations can be visualized, achieved through, for example, a previously induced gene... your marker of interest. Indirect labeling is achieved through protein-tag or protein-protein interactions...

  4. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...pseudopregnant female mice. If you obtained the desired chimeric mice, you would breed them many times, hoping ...line with a recombinase. If it turned out your chimeric mice did not contain edited germ cells, your experiment...inject the ES cells into a mouse embryo to create chimeric mice. This laborious process takes ~8-10 months...When delivering the ssDNA template and CRISPR machinery, Quadros et al. recommend ribonucleoprotein (RNP...

  5. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...have created many engineered serotypes by mixing, matching, and modifying capsid and genomic structures. ...., Brown, K. E., Walters, R. W., Zabner, J., & Chiorini, J. A. (2001). Adeno-Associated Virus Serotype...Kuleshova, E., Ojala, D., Huang, C.-C., Gerfen, C. R., Schiller, J., Dudman, J. T., Hantman, A. W., Looger, L....M., Keshavjee, S., Brown, K. E., Welsh, M. J., Chiorini, J. A., & Zabner, J. (2001). Binding of adeno-...

  6. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...engineered from the cmt and cym bacterial operons. A chimeric transactivator, cTA, was created by fusing CymR...the system, researchers have used split GAL4 architecture or combined the system with Flp/FRT, as reviewed...) to GAL4 or VP16 activation domain results in chimeric GAL80-repressible and GAL80-insensitive activators...lethality in flies. Riabinina et al. also developed chimeric transactivators GALQF and LexAQF, which activate...

  7. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...In an era where millions of people hold a PhD, achieving our career goals can be a real challenge. On top... hinder them from advancing their careers and achieving success.” Pedro Gonzalez Menendez (Mentee IMP ...compared to their pre-program self-assesment. Achievements directly attributable to participation in the...interested in breast cancer and metastasis as well as teaching and mentoring activities. Follow her on twitter...

  8. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...optimal/minimal injection titer and volume needed to achieve adequate expression in your cells of interest.  ...waiting periods and/or higher injection titers to achieve adequate expression. Conducting a time course experiment...to minimize off-target expression while still achieving adequate expression in your cells of interest....different brain region that the same result will be achieved. The fact is that there are many known and unknown...

  9. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...2. Fluorescence Activated Cell Sorter (FACs) A machine that sorts cells according to whether or not they... deflect them into appropriate containers. The machine is useful for distinguishing cell populations that...21596592. PubMed Central PMCID: PMC3164316. 5. Chieppa, Marcello, et al. "Dynamic imaging of dendritic...165. PubMed PMID: 18193049.  9. Filipe-Santos, Orchidée, et al. "A dynamic map of antigen recognition ...

  10. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...2017 contest winners:   Chicagoland's Community Biology Lab (@ChiTownBio) and one of our favorite twitter...holiday cheer, and we loved it! Check out their matching "MACK ATTACK!" holiday sweaters. Alex Dainis ...

  11. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...business efforts at Addgene as the Chief Financial Officer and Chief Operating Officer. Prior to Addgene... Director of Technology for 7 years, and is now Chief Software Engineer at Ginkgo Bioworks. He also holds...

  12. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    ...various cloning methods and tools to construct chimeric cellulases that enable E. coli and Citrobacter...plans for these plasmids have a heavy focus on teaching. He hopes to teach his molecular biology students...Diseases Read About Some of Our Outreach Efforts in China Additional Resources on Addgene.org Learn About...

  14. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    ...would be a big deal if it worked out.”- Melina Fan, Chief Scientific Officer “Choose your PhD lab carefully...opportunity.” - Sophia Cheng, Senior Software Engineer “Searching for a new job can be an imposing endeavor.  Break...the place that you are applying.” - Melina Fan, Chief Scientific Officer If there’s a career topic that...

  15. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    ...therapy, Sacher and Zheng also saw the need to archive phage reagents. If a phage researcher retires, ...likely that the phages are lost if they are not archived. “It’s very sad because it’s years of work,” Sacher... it can also fill this role by cataloging and archiving information on individual phages. Integrating ...

  16. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    ...method that uses iterative staining and chemical bleaching to allow for high-resolution imaging without degrading... Yaniv, Z., Yao, L., Marr, J., Beuschel, R. T., Ichise, H., Gola, A., Kabat, J., Lowekamp, B., Speranza...IBEX: An iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues...

  17. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...constructing 14 luciferase donor Rluc8-Gα chimeras, four acceptor GFP2-Gγ chimeras, and two untagged Gβ constructs...or drug. In the inactive state, the luminescent chimeric protein Gα-Rluc8 and Gγ-GFP2 will be in close ...

  18. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    ...in the educational sphere, it can range from researching an area, to writing an introduction that gives...mitosis and meiosis in biology. Lots of it is researching the different aspects of the subject you're writing... Keown, a staff writer at the non-profit Fred Hutchinson Cancer Research Center. And make sure to subscribe...

  19. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...turn up the heat when there is a bad Northeastern chill. Check out Joanne's Reddit AMA  Few of us spend... in this country and we are quick to throw out chipped or imperfect equipment.  We can’t safely recycle...illustration above) and bribed the guys in the hospital machine shop with coffee and donuts to cut the pieces of...
Showing: 201 - 220 of 648 results