We narrowed to 582 results for: Cit

...Receptors) CAR T cells and PD-1 modulators. An exciting development in immunotherapy is the combination...model for this study (Ref 6)). In addition, it was exciting to see that the use of optogenetic tools is expanding...observe the function of immune cells that are not excitable but responsive to calcium. We’d love to expand...

... will be necessary to improve its multiplexing capacity. Multiplexing is key due to the natural redundancy...As nucleases may have off-target effects and/or toxicity, it would be advantageous to instead use a preexisting...multiplexing in systems with limited packaging capacity. Still confused about the various tRNA expression...

... delivery mechanism, but their small packaging capacity of 4-5 kb limits the delivery of larger transgenes...spatial precision of gene expression and reduces toxicity as compared to previous Cre/lox or drug-induced...inactivating Cre. This is critical for reducing Cre toxicity in long-term experiments.  Figure 4: The...

...At Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse...Transcriptional Devices Dependent On GFP). In testing the specificity of their system, Tang et al. found that YFP and... using the rTetR system, but Cre-DOG lacks the toxicity that can be seen with high levels of transcription...

... blue light (Bubeck et al., 2018). 3. Reduce cytotoxicity of CRISPR-Cas editing in tissues The small size... systems prevents the off-target effects and cytotoxicity that result from excessive nuclease activity...Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem Cells. Molecular Therapy...

...rate (Redfern et al., 1999) or altered neuron excitability (Zhu et al., 2014). The system allows you to...important to consider that the same receptor can elicit different responses depending on what cell type... chemogenetic receptors can be used to alter excitability by lowering or raising the threshold for signaling...

...antibody’s specificity. Strength and accuracy The strength is based on binding specificity and exposure...public service to the science community, and I am excited to be a part of it! If you are interested to get...

...on the position in the protospacer, the editing capacity of the ABE8s ranged from 1.5x higher for positions...For applications that require high DNA editing specificity, for example for potential therapeutic approaches...ABE8s demonstrate an overall improved base editing capacity, even at sites that used to be difficult to target...

... A major drawback of this method is the stray excitation of neighboring neurons in opsin dense regions...activity, and protein-protein interactions. We’re excited to see how you adapt similar FLINC biosensors to...dark yellow fluorescent protein (YFP) with an excitation peak of 519 nm. These proteins have improved ...

...trials, wild type AAV is not associated with any pathogenicity and has a low immunogenic profile. AAV was first...new and refined forms of capsids with improved specificity. AAV vectors are able to facilitate and enhance...abilities to infect target tissues. Improved specificity through control of gene expression and the selection...

... the Science City The conference was located in the modern, relatively new “Science City” area on the...choose to locate there. I made time to go into the city of Guangzhou and see some of the sites. Most fascinating...

...this, there are close to 10,000 citations for anti-Myc 9E10 in CiteAb, many of which are from this year...Locate a promising monoclonal antibody with high specificity. (2) Narrow down the specific epitope that your...

...license. If you’re currently using mVenus or mCitrine, or if existing yellow fluorescent proteins just...binds to AMPA receptor subunit GluA1 found at excitatory synapses (Rivera & Huang, et al., 2025). AF was...determined cells. This version of ATLAS works only at excitatory synapses; however, its modular design allows ...

...moments. The models function as thinking tools – eliciting questions like “what does this model representt...proteins for researchers. Modeling CRISPR We were excited when Jacob Corn contacted us in 2014 to ask if ...in a rationally engineered Cas9 with improved specificity. Hold this flexible model in your hand for a ...

...light by using a pinhole.  However, the excitation light excites all of the fluorophores as it passes through...order to improve axial resolution and reduce phototoxicity for imaging live specimens, they combined light...

...history was very much in the making. One of the exciting, and challenging, aspects of talking to 8th graders... proficient in science, regardless of race or ethnicity. Perhaps you don’t have children but are interested...strawberry, or demonstrated that they’re living in an exciting, tumultuous time in scientific history. Whatever...

...application and submitting it, to evaluating the capacity of an organization to receive and manage funds... was moved from one department to another within city government. Throughout these moves and program changes... our program didn’t stay in one place within the city infrastructure. My career path changed when I was...

...community is no small task. The EDGE program solicitation requires funded labs to rapidly disseminate ...programs for K-12 students. You can see why I was excited to be invited to attend. My job is to ensure that...marmorated stink bugs to make them pink. We’re excited to see new tools being developed and shared for...

...plasmids, optimized for a variety of species.   Specificity concerns? If off-target effects are a worry, ...nickases (Cas9n) to the target locus, enhancing specificity over a single SpCas9. The drawback of this approach...and consider using a Cas9 variant with higher specificity (eSpCas9 and HypaCas9) instead.  Choosing a Cas9...

...targeting of specific cell types, it has a limited capacity to carry large DNA cargos. As a result, the search...focus in recent years. TnpB nucleases represent an exciting class of compact genome editors, typically only...limitation of viral vectors is their restricted cargo capacity, which makes it difficult to accommodate the large...