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There are many plasmid tools available in molecular biology so how do you chose the right cloning method for you? Here, consider speed, cost, difficulty, efficiency, and the number of inserts when designing your cloning strategy.

Learn how a 3D protein model can help you visualize, learn, and teach others about protein structure and function.

Learn more about synthetic biology resources available at Addgene. Browse featured collections or depositing labs.

Our depositors don't just deposit great plasmids - they also do some incredible science! Check out some incredible depositor research stories from 2022.

Announcing Addgene's AAV Data Hub! The Data Hub is an open platform for scientists using AAV to share practical information about their experiences.

Using anti-mouse antibodies in a mouse model can result in high background signal. Here are three ways to overcome this challenge.

Multiple antibodies often exist for a single target protein, allowing for the targeting of different epitopes as protein conformation changes.

Find details on five methods to quantify DNA: UV absorbance, fluorescence dyes, agarose gel electrophoresis, capillary electrophoresis, and diphenylamine.

Scientists are now using Addgene's plasmid repository as a source of sequence data sets. Researchers from MIT built a classifier that predicts whether a gene sequence is natural or synthetic and found that more and more genes are being sourced from farther away in the tree of life.

Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit.

Find tips to help you write you next cover letter and land your dream career.

A review of the common antibiotic resistances genes used in bacteria.

Find tips on how to write your first review article - a useful experience for any scientist.

Learn all about mesothelioma and upcoming immunotherapy treatments for this devastating form of cancer.

Addgene interviews Hodaka Fujii of Osaka University to learn about his lab's enCHIP system, their new CRISPR tools, community involvement, and more.

Multicistronic or bicistronic vectors contain IRES and/or 2A peptides to allow for coexpression of multiple genes from a single transcript.

Use this transfection calculator when packaging your AAV vectors.

Halloween is a favorite holiday at Addgene. As we look forward to this year's Halloween festivities, check out past costumes from our Addgenies.

Browse Addgene's COVID-19 collection of SARS-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids for viral pseudotyping with SARS-CoV-2 spike protein.

Drive-and-proccess (DAP) arrays are a new tool from the Gao lab that allows for up to 31-loci CRISPR multiple base editing.