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We narrowed to 824 results for: EED

Showing: 201 - 220 of 824 results

  1. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... and by offering advice on areas where you might need some help. Today: Career Planning Resources. The...be a graduate student or postdoc forever, and you need to be thinking about what comes next. To that end...attending one of these fairs is a great start, but you need to do more than just show up. Do your homework and...employer in mind, you may consider starting with indeed.com or monster.com, where you’ll find a variety of...hope to have a career outside of academia, you may need to look a bit harder to find your mentor. This may...even be a colleague. A mentor does not necessarily need to be someone a lot older than you are; try using...

  2. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ... of each plasmid needed for the whole experiment Total volume (μL) of plasmid needed for the whole experiment...specific molar ratios. To get these ratios right, you need to do a few calculations. This calculator can do...will calculate the following μg of each plasmid needed per dish or stack. Calculations are based on using...equation the calculator uses to give you the μl needed of each plasmid. Each part of the equation is labeled...

  3. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ... in almost all organisms and cell types, (ii) no need to add a co-factor, (iii) easy visualization with...EGFP variant, mEGFP (Zacharias et al., 2002). GFP needs oxygen Chromophore formation requires a fully folded...specific biological system under anaerobic conditions needs to be examined on a case-by-case basis. GFP is sensitive...light by a chemical reaction and hence, there is no need to excite the sample. Although bioluminescent probes...for biological spectroscopy and microscopy." Proceedings of the National Academy of Sciences 114.23 (2017... real-time multicolor luminescence imaging." Proceedings of the National Academy of Sciences (2015): 201418468...

  4. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...ll cover the normalization and loading controls needed for relative quantification of a western blot —... it’s overkill in many cases. Often, you may not need to know the exact quantity of your POI, but simply...normalization is a nearly ubiquitous tool, but proceed with caution! Loading controls only address one...your context. If you don’t find anything, you’ll need to conduct a control experiment to demonstrate that... in a separate step from the POI blot, you don’t need to worry about the linear detection ranges of multiple...increasing concentration of β-actin in panel A. Indeed, the quantification in panel B is not especially...

  5. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...narrative. Scientists do this all the time, you just need to figure out how to make it suspenseful. 4. Start...your talk early - like today Don’t wait until you need to speak to start creating slides or thinking about...will be familiar with the terms. If you feel you need to define a term, but are worried that this might...but not reading the explanation. The people that need the information will find it. Experienced speakers...mentoring groups to practice giving talks and getting feedback. Wouldn’t you rather hear it from your peers than... many things. One good topic or story is all you need, if you do it well.  Resist the urge to show all...

  6. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    ... scientists by bringing you all the plasmids you need. We're focused on our mission – documenting your... for scientists everywhere to get the tools they need to progress their research. *2015 total reflects... our growing community! Our plasmid repository succeeds because of our amazing community. The trust of...transfer offices that facilitate MTAs. The great feedback from scientists who request plasmids and use our...from our blog readers. You all drive Addgene to succeed and to improve, so thank you. 500,000x Thank You...

  7. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...do you decide which tools to use, and what do you need to know about them to be successful in your experiments...to activate or inhibit specific neurons without needing to consider secondary signaling pathways. There... of tool appropriate for your experiment, you’ll need to find a specific tool. There are many, many options...times or frequencies (Zhang & Cui, 2015). You’ll need to test for these differences when designing your...previously published system in your own work, you’ll need to know (and understand!) the hardware types, construction...light-gated cation-selective membrane channel. Proceedings of the National Academy of Sciences, 100(24),...

  8. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...have been accumulated over the years using them. Indeed, the future of mouse work is bright as it is now... a mouse model, and its success is not always guaranteed. To learn more about this process check out our...Part 1: Genetically Engineered Mice and Part 2: Breeding and Crossing Mice. My first CRISPR mouse experiment...oligonucleotide or plasmid as the repair material if needed (for knock in models). CRISPR mouse model basics...directly into fertilized mouse eggs, circumventing the need for enrichment and selection offered by mouse ESCs...transmission of the transgene, this technique is still in need of improvement. Also when working with CRISPR, one...

  9. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...generation of tools had limited sensitivity and speed and were only available as green probes. The Patriarchi...important because alpha2-AR can provide inhibitory feedback in response to norepinephrine release, and clinically-used...however, be studied with the nLight indicators. Speed One of the major advantages of this next generation...generation of norepinephrine sensors is their speed. nLightG is activated eight times faster than GRABNE and...concentrations of dopamine. This cross reactivity will need to be accounted for in your experimental design....

  10. Antibodies 101: Validation

    Type
    Blog Post
    ... are not convinced by the existing data, you may need to roll up your sleeves and perform some extra validation...that it will perform as expected in another. You’ll need to validate that points 1 and 2 hold true under ...different tissues or cell types. If your antibody indeed binds to your target then you would expect to see...expression and non-specific, background signal, so you’ll need a range of expression strengths to be confident ...unexpected. This approach has high specificity, but you need to be aware of any possible alternative translation...identified as your target protein, allowing you to proceed with confidence.  Share your results Ultimately...

  11. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...Martian chicory root, but considering this is a needle in a haystack type of objective, it was surprisingly...delivery Overview: Before any editing can begin we need our A549 cells to express Cas9. In these particular...quantify Cas9 activity using this assay, you really need to use FACS (like they did in the original publication...could be easier to visualize and identify). If you need/want to put the digestion site in the middle of ...highlights the important results (for example, if you needed to make a figure with the data). Using acrylamide...an edit. I didn’t do this, but to do it you would need a pretty good gel image. If you are analyzing short...

  12. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...information about. The assumptions and requirements that need to be met for a parametric test are as follows:   ...test as the standard value has no variation that needs to be accounted for. ANOVA tests ANOVA tests are...these tests. All sample comparisons Do you really need to know every pairwise comparison within your population...samples are assessed. However, if many comparisons need to be performed, Bonferroni and Benjamini will greatly... appropriate to use if all sample comparison is needed, without a high number of samples (~5). Tukey’...instead of after you’ve already run it.) You don’t need to be a mathematician to select the right statistical...

  13. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    ...mastery. Scientists need to be doers for a long time before they master the skills they need to be successful...these three roles allows you to identify what you need to do to be successful in a specific role or cycle...hands-on bench work. To really be happy, you may need to find a way to do, manage and lead all in one ... opportunity to take on the role of leader, one needs to take the initiative by bringing and effecting...

  14. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...easy to find in publications, so this is a much-needed community resource. Instead of painstakingly searching...use the AAV Data Hub to quickly find details they need for planning their next experiments using a specific...image yourself and it is under copyright, you will need to obtain written approval from the copyright owner... that this provides a clear overview of what you need to submit data. Please note that the data hub is...after submitting or viewing the data you have any feedback at all, we’d appreciate you sharing it through...

  15. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

    Type
    Blog Post
    ...fertilizer, seeds and a growth space with fluorescent lights. Other experiments require only seeds, petri ... to collect, preserve, reproduce and distribute seeds, DNA and other resources for Arabidopsis and related... free-of-charge from the ABRC Outreach website. Seeds, which are free for K-12 teachers and available ...require a huge investment in resources. The materials needed to conduct the experiments outlined in ABRC’s kits...

  16. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type
    Blog Post
    ...Initiative, will allow Addgene to create a much needed open-access recombinant antibody/affinity reagent...produced in animals may be variable among animals and bleed dates. The development of monoclonal antibody (mAb...neuroscience research today, but there is a critical unmet need for an open-access, molecularly defined, and well...Trimmer. But in order to create such a resource, we need your help. You can participate by depositing your...share, edit, and access the affinity reagents they need to make their next big discoveries. We welcome you...

  17. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ... be a huge challenge. Epitope tags eliminate the need for target-specific antibodies and have been widely...fortunate enough to study actin, then you may not need to read this - but for many other proteins, quality...tracking your protein may also be possible, without the need for an antibody. Tags can enable the separation ... Myc The Myc tag is a 10 amino acid peptide (EQKLISEEDL) derived from the protooncogene of the same name...interfere with protein function and localization. If you need monoclonal GFP antibodies or guidance on designing...

  18. Plasmid Cloning by PCR

    Type
    Blog Post
    ...cloning process - please check out that post if you need a more detailed refresher on the downstream steps...restriction site to the reverse primer. Next, we need to examine the DNA sequence that we want to amplify...the Reverse Primer, the design is similar, but we need to use the reverse complement to get PCR amplification... (30bp with 18bp of homology to the ORF). We now need to generate the reverse-complement of this sequence...wide range of annealing temperatures, but you may need to increase your primer length and increase the ...

  19. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ... bottom line is that a lot of AAV particles are needed to get a few good ones into a cell to produce expression...transport and transgene expression It’s clear that we need high titers, as contact between the cell and any...protein. However, expression of receptors may not need to be as high to achieve an observable signal in...in your experiment, therefore titer may not need to be as high when delivering receptor constructs with...improve AAV production.     References 1.Clark, K. Reed. "Recent advances in recombinant adeno-associated...

  20. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...will also need to be perfected until the efficiency of repair approaches 100 percent. “We need to be sure...currently being tested in clinical trials. It is speeding the process of the discovery/development of genetic...advantage over induced pluripotent stem cells, which need to go through a phase of crisis where they 'collect...effects are important,” she said. “We also really need to get a handle on how cleavage sites are repaired...
Showing: 201 - 220 of 824 results