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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 447 results for: ENA;

Showing: 201 - 220 of 447 results

  1. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...Proper controls provide the constant variables that enable the correct interpretation of the effect of the...transfection control measures transfection efficiency and enables observation of any effects of the transfection ...

  2. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...expression promoter from glyceraldehyde 3-phosphage dehydrogenase Constitutive  Very strong, also called TDH3 ... expression mRNA Yeast promoter for alcohol dehydrogenase I Repressed by ethanol Full length version is...

  3. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...The modular system of linkers and spacers could enable many future variations using nanobodies or scFvs...before vitrification, they can be completely denaturated (left) or complexes may dissociate  (right). ...

  5. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... embryonic development, extended-open integrins enable tissue morphogenesis, organ development, and the...integrins demanded higher tensions before they would enable cells to spread, resulting in cells that elongated...

  6. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...demonstrated excellent counter-selection which enabled very efficient recombineering.     We were inspired...CRISPR/Cas9 system with lambda red recombineering enables simplified chromosomal gene replacement in Escherichia...

  7. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ... or drug selection, assuming your reagents are amenable to this. If delivery isn’t an issue, you may need...M, Schönig K, Eckert H, Eschstruth A, Mianné J, Renaud J-B, Schneider-Maunoury S, Shkumatava A, Teboul...

  8. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...produce BV, an additional gene coding for heme oxygenase should be supplied (Baloban et al., 2017).  It...multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET. Nat Chem Biol 14, 591-600...

  9. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...authors use affordable, field-ready, Bluetooth-enabled PCR machines that can even be battery operated,...actively interested in science communication and enabling access to science in developing countries. You...

  13. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...ELISA and cell surface display are common. ELISA enables quantitative measurement of antigen-antibody interactions...mutant libraries on the surface of cells. This enables direct screening via flow cytometry for improved...

  15. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... al., 2008), based on a bacterial haloalkane dehalogenase that binds a synthetic chloralkane (CA) ligand...one self-labeling tag and a fluorescent protein) enables applications like multi-color imaging (Figure 2...

  16. Antibodies 101: Validation

    Type
    Blog Post
    ...manipulation. Additionally, not all sample types are amenable to genetic manipulation, nor can all genes be ...antibodies for immunocapture applications and is amenable to high-throughput analyses, however, it requires...

  17. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...Although genome editing technologies have brought a renaissance in the field of health sciences, energy, and ...533:420–424 . https://doi.org/10.1038/nature17946 Lenay C (2000) Hugo De Vries: from the theory of intracellular...

  19. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...-bonded oxygen. This simple enzymatic reaction enabled conversion between the purine bases (A and G) or...., & Zhang, X. (2021). Glycosylase base editors enable C-to-A and C-to-G base changes. Nature Biotechnology...

  20. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...plasmids. Each destination vector contains GFP, enabling you to select cells with high GFP expression. ...This kit is built for serious multiplexing and enables users to express up to 7 gRNAs. The kit contains...
Showing: 201 - 220 of 447 results