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...pic.twitter.com/X6QYXteRLT — Nika Heijmans (@nikaheijmans) December 21, 2018 Eppi the red nosed reindeer from @ladiesinlabcoats....co/MYMnXicWqb — Matthew J. Sikora (@mjsikora) December 18, 2018 Fireplace from the Dyer Lab If you’re...pic.twitter.com/bDRqAKrWHz — Dyer Lab (@DyerLab_Emory) December 14, 2018 Thanks to all who participated in ...

...them. Collectively, the pool of CRISPR software embodies solutions that facilitate most experimental applications...in the table): Basic functions: These functions embody the main goals of the software and should be the...genomes are denoted by organism name and genome assembly version. The names in the table appear as they...organisms and will help you whether you have a full assembly or not. It is an offline tool, but comes with ...

...of DMEM complete instead of a viral dilution). Remember, this is an additional 10-fold dilution of the...

...meetings include all collaborators, from the lab members who are conducting the experiments to the PIs. ...closely replicates how you’d interact with team members in person, and improves overall communication.  ... informal communication you’d likely have with members of your lab that you see in person, and helps improve...

... the pores being opened in the pathogen’s cell membrane (Dunkelberger & Song, 2010). As cells cannot survive... survive when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody...providing structure and isotype determination, remember that it can do so much more than that!    References...

...This grows our relationship with our community members. We also find that, as a nonprofit, we attract ...is strength in diversity and in empowering team members to take initiative and give their best As we continue...Research Listen to Our Interview with Addgene Board Member, Connie Cepko Resources on Addgene.org Learn ...

...characteristics like pH, redox state, and voltage across membranes. These sensors generally follow the same pattern...an organelle-specific targeting signal or a transmembrane domain, biosensors can be targeted to a particular...endoplasmic reticulum (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted...

...pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]) of this membrane-embedding voltage detector. Find Archon1 and its cre-dependent...GABA sensor (pAAV.hSyn-FLEX.iGABASnFR) has good membrane localization and brightness. The F102G variant...

...& Biology Society (EMBC), 4322–4325. https://doi.org/10.1109/EMBC44109.2020.9175600 Park, S., Loke, G....who together developed multifunctional fibers, embedded, and validated simultaneously in the brain and...

...10,000 users per month in November 2014 to ~30,000 users per month in November 2015. We will continue ...providing recorded interviews with Addgene community members. In addition we’ve experienced incredible growth...

...Tat. Although CPP fusions can translocate a cell membrane, the presence of the CPP may alter protein function...various loxP reporters. In both mouse and human embryonic stem cells, they found very high recombination...neuronal and gut stem cells, dendritic cells, embryonic fibroblasts, glia cells and neurons, and cell ...

... out these questions ahead of time so that lab members have time to prepare their thoughts. Then at the...Set up a brief bi-weekly meeting where all lab members talk no science. No data presentation will appear...remotely in science are endless. Let’s adapt and embrace new ways of working, reconnect as human beings ...

...editing system to correct the cystic fibrosis transmembrane conductor receptor (CFTR) by homologous recombination...of CRISPR/Cas9." Cell Stem Cell. 13, 659-662 (5 December 2013). 8. Gerald Schwank et al. "Functional Repair...Fibrosis Patients." Cell Stem Cell. 13, 653-658 (5 December 2013).    ...

...differentiation, the Zhang lab transduced human embryonic stem cells with the viral TF library, with each...lentiviral particles and used to transduce human embryonic stem cells. Transduced stem cells were differentiated...this screen successfully differentiated cells resembling all three germ layers (ectoderm, endoderm, and...

...Institute for Biologically Inspired Engineering and member of the Addgene Advisory board, on the difficulty...expressing Cas9. As John Doench (Broad Institute and member of the Addgene Advisory Board) says: "When you'... running. Both Alex Chavez and John Doench are members of the Addgene Advisory Board. Additional Resources...

...everyone gets a chance to talk and less vocal team members still have their voices heard. Here's a template...of the agenda, and assign each one using group members’ initials. If appropriate, email this around after...to help keep you accountable for moving along. Remember, many of the more in-depth discussions can happen...

...and improving accessibility on our website.  As members of Addgene’s content team, we — Jo, Brook, and ... at the Society for Neuroscience conference in November 2023, we hosted our very first professional development...clear writing Let’s use Figure 1 as our example. Remember, our goal is to use words to describe the image...

...viral envelope, which fuses with the target cell’s membrane, allowing the viral particle access into the cell...inside the nucleus, without the need for envelope disassembly. Both lenti and retro viruses can accommodate...of murine leukemia virus DNA depends on mitosis.” EMBO J. 12 (1993) 2099-2108. PubMed PMID: 8491198. PubMed...

...adjunct faculty member) have together been working toward this goal for years as members of the ENCyclopedia...the second plasmid can be done in one day in one assembly reaction. This second plasmid saves you a couple...

...and names, swapped leadership, and grown to 25 members.  What is Sample6? Sample6 is an enrichment-free...technologies, effectively communicating the advantages embodied, and driving the enterprise value of Sample6.  ...Read On! Synthetic Biology & the Importance of Assembly Standards Story of a SynBio Startup: RevBio's ...