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We narrowed to 444 results for: HAL;

Showing: 201 - 220 of 444 results

  1. Bioinformatics at Addgene

    Type
    Blog Post
    ...300,000 individual nucleotide sequences. More than half of these sequences were generated by Addgene as ...on-site, but now we were presented with a new challenge: automating the bioinformatics steps needed to...

  2. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...suited for recombinant protein expression. Many challenges can arise when over-expressing a foreign protein...functional RNaseE which results in longer transcript half-life F- ompT lon hsdSB (rB- mB-) gal dcm rne131...

  3. CRISPR Guide

    Type
    Guide
    ...less widely used in bacteria due to technical challenges, bacterial CRISPR libraries have been developed... S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., Cradick, T. J., Marraffini, L. A., Bao, G....., Gootenberg, J. S., Kriz, A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., Koonin, E. V., Sharp...790. PMID: 38216671 Klompe, S. E., Vo, P. L. H., Halpin-Healy, T. S., & Sternberg, S. H. (2019). Transposon-encoded...Communications . 9 (11), e1007749. PMID: 30575746 Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott...cells. Science . 343 (6166), 84–87. PMID: 24336571 Shalem, O., Sanjana, N. E., & Zhang, F. (2015). High-throughput...Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall, E. M., & Myers, R. M. (2015). CETCh-seq: CRISPR...

  4. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...double strand break repair, it has proven more challenging to develop high efficiency CRISPR-based approaches...delivering CRISPR components as RNPs and delivering two halves of a repair template spread across two AAVs, it...

  6. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...and significance, these receptors have proved challenging to study. Cells often express multiple integrins...adhesion. The results surprised the investigators, challenging the long-held belief that αVβ3 would be the most...

  7. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ... the body or self antigens, and toxins that are lethal to animals.  In the clinic, scFvs’ size also provides...plasmid in bacteria (Ahmed et al., 2012). One challenge of bacterial expression is the proper formation...

  14. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...transduction of lung cells via airway administration (Halbert et al., 2001). It additionally has tropism for ...-0. https://doi.org/10.1016/j.cell.2025.06.026 Halbert, C. L., Allen, J. M., & Miller, A. D. (2001). Adeno-Associated...

  15. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...nn1525 Gunaydin, L. A., Yizhar, O., Berndt, A., Sohal, V. S., Deisseroth, K., & Hegemann, P. (2010). Ultrafast... Augustin, S., Sauter, K., Papagiakoumou, E., Gottschalk, A., Soba, P., Emiliani, V., Engel, A. K., Hegemann...

  16. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...important and abundant neurotransmitters, but it is challenging to monitor because only a small number of glutamate...to specific cell types in the brain can be a challenging — but critical — factor for successful experiments...

  18. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...CRISPR/Cas9, TALENs, and ZFNs). However, several challenges still remain, such as making gene edits completely...nuclease technology with the piggyBac™ system, these challenges can be considerably reduced. The piggyBac™ transposon...

  19. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ... genomic DNA to amplify the gRNA targets was challenging because you’re limited by the sequence of the...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. ...

  20. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...molecule of GFP. Because red light has approximately half the energy of blue light, two photons of red light...model embryos >15 μm Thsee are some of the most challenging samples to image as they require both optical...
Showing: 201 - 220 of 444 results