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Showing: 201 - 220 of 430 results

  1. Sequencing Primers

    Type
    Guide
    ... forward primer pCasper-F GGGTTTTATTAACTTACAT (Vosshall lab) 5' end of Drosophila mini-white gene, reverse...

  2. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...and significance, these receptors have proved challenging to study. Cells often express multiple integrins...adhesion. The results surprised the investigators, challenging the long-held belief that αVβ3 would be the most...

  3. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...double strand break repair, it has proven more challenging to develop high efficiency CRISPR-based approaches...delivering CRISPR components as RNPs and delivering two halves of a repair template spread across two AAVs, it...

  5. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ... the body or self antigens, and toxins that are lethal to animals.  In the clinic, scFvs’ size also provides...plasmid in bacteria (Ahmed et al., 2012). One challenge of bacterial expression is the proper formation...

  9. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...important and abundant neurotransmitters, but it is challenging to monitor because only a small number of glutamate...to specific cell types in the brain can be a challenging — but critical — factor for successful experiments...

  12. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...nn1525 Gunaydin, L. A., Yizhar, O., Berndt, A., Sohal, V. S., Deisseroth, K., & Hegemann, P. (2010). Ultrafast... Augustin, S., Sauter, K., Papagiakoumou, E., Gottschalk, A., Soba, P., Emiliani, V., Engel, A. K., Hegemann...

  14. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...CRISPR/Cas9, TALENs, and ZFNs). However, several challenges still remain, such as making gene edits completely...nuclease technology with the piggyBac™ system, these challenges can be considerably reduced. The piggyBac™ transposon...

  15. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ... genomic DNA to amplify the gRNA targets was challenging because you’re limited by the sequence of the...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. ...

  17. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    ...molecule of GFP. Because red light has approximately half the energy of blue light, two photons of red light...model embryos >15 μm Thsee are some of the most challenging samples to image as they require both optical...

  18. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...then flow cytometry can help you overcome these challenges and make these types of experiments easier and...fluorophores Fluorescent antibodies get around the challenges of fluorescent protein fusions by binding to ...

  19. Chemogenetics Guide

    Type
    Guide
    ...Ikefuama EC, Slaviero AN, Silvagnoli AD, Crespo EL, Schalau R, Gott M, Tree MO, Dunbar GL, Rossignol J, Hochgeschwender...

  20. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...replicates, spreads, causes neurotoxicity, and is lethal if left untreated (for review see Dietzschold et..., Harris, J.A., Ng, L., Winslow, B., Cain, N., Mihalas, S., Wang, Q., Lau, C., Kuan, L., Henry, A.M., ...
Showing: 201 - 220 of 430 results