We narrowed to 327 results for: INO

1. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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   Blog Post

   ...active up to 100°C.  The IgnaviCas9 protein is 1,241 amino acids long, has optimal sgRNA spacer length of 22...

2. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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   Blog Post

   ... translation in U2OS cells. HA is small, only 9 amino acids, thus it is translated quickly by the ribosome...

3. Summer SciComm Series: Master of SciComm

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   Blog Post

   ...once again write for fun eventually saw an English minor tacked onto my BSc. With this decision I had unwittingly...

4. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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   Blog Post

   ...affect editing efficiency. Smaller size At only 887 amino acids in length, NgAgo is shorter than SpCas9 (1368...

5. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

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   Blog Post

   ...into the E. coli chromosome in some cases. This terminology held until the 1960s when scientists began to...

6. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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   Blog Post

   ...just 1 minute of exposure. MG-2I-dL5** experienced minor bleaching after longer exposure times. Additionally...

7. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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   Blog Post

   ... enhanced “pdDronpa” domains could be placed in amino acid loops on either side of an enzyme’s active ...

8. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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   Blog Post

   ...pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol anchor. SGZ contains a PDGFR transmembrane...

9. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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   Blog Post

   ...professionals, joined the platform; while they are a minority, their help has been essential to push certain...

10. What Good Citizenship Can Do for Reproducibility in Science

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    Blog Post

    ... can never completely eliminate the risk that a minor variation in experimental conditions will derail...

11. New and Upcoming Viral Vectors - September 2019

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    Blog Post

    ...(105322-AAV1) GABA sensors Sensing levels of g-Amino butyric acid (GABA) - the primary inhibitory neurotransmitter...

12. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    Blog Post

    ...lines. Lastly, the Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor, sufficiently suppresses basal...

13. Summer SciComm Series: A PhD in Science Communication

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    Blog Post

    ...scientific methodology makes it easier to spot even minor weakness or limitations in a piece of research (...

14. Plasmids 101: Gram Negative and Positive Bacteria

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    Blog Post

    ...University, who is majoring in marine biology and minoring in studio arts photography.  References and resources...

15. Components of CRISPR/Cas9

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    Blog Post

    ...aureus (SA); SaCas9 3' NNGRRT or NNGRR(N)  Acidaminococcus sp. (AsCpf1) and Lachnospiraceae bacterium ...

16. Protocol - pLKO.1 – TRC Cloning Vector

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    Protocol

    ...Sigma #A9539 D.2 Screening for Inserts Day 1: 1. Inoculate 5 colonies from each ligation into LB + 100 μg...

17. Why and how to start studying chromatin (with Addgene's help)

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    Blog Post

    .... 2017 Aug 24;170(5):956-972.e23 Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex...

18. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    Blog Post

    ...The mixed PCR and incomplete PCR methods yield a minority of usable product with two 5′ overhangs, so these...

19. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    Blog Post

    ...presented study analysed a model of hepatocellular carcinoma, a cancer which can develop from a state of chronic...

20. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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    Blog Post

    ...fluorescent proteins, differing by only a single amino acid. Without further information about properties...