We narrowed to 326 results for: INO

1. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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   Blog Post

   ...pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol anchor. SGZ contains a PDGFR transmembrane...

2. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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   ...just 1 minute of exposure. MG-2I-dL5** experienced minor bleaching after longer exposure times. Additionally...

3. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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   ... enhanced “pdDronpa” domains could be placed in amino acid loops on either side of an enzyme’s active ...

4. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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   ...professionals, joined the platform; while they are a minority, their help has been essential to push certain...

5. Components of CRISPR/Cas9

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   ...aureus (SA); SaCas9 3' NNGRRT or NNGRR(N)  Acidaminococcus sp. (AsCpf1) and Lachnospiraceae bacterium ...

6. SARS-CoV-2 Pseudotyped Virus

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   ... optimization, deletion of the C-terminal 18-21 amino acids and a D614G change which is present in many...

7. CRISPR Plasmids and Resources

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   ...Plasmids and Resources CRISPR technologies are continously developing and expanding. Addgene works with ...

8. CRISPR Plasmids - Base Edit

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   ...editors have been engineered to convert adenosine to inosine, which is treated like guanosine by the cell, creating...

9. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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   ...lab. Chemicals like calcium phosphate and diethylaminoehtyl (DEAE)-dextra neutralize or even impart an...

10. Why and how to start studying chromatin (with Addgene's help)

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    .... 2017 Aug 24;170(5):956-972.e23 Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex...

11. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    ...The mixed PCR and incomplete PCR methods yield a minority of usable product with two 5′ overhangs, so these...

12. Antibodies 101: Isotypes

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    ...have different versions or subclasses that have minor differences in their heavy chain constant domains...

13. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    ...presented study analysed a model of hepatocellular carcinoma, a cancer which can develop from a state of chronic...

14. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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    ...fluorescent proteins, differing by only a single amino acid. Without further information about properties...

15. Sharing is Caring: But How to Distribute Open Hardware?

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    ... available tools such as the Raspberry Pi, the Arduino, or 3D printers are key to enabling this work. ...

16. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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    ...phage recombination machinery is induced using L-arabinose. These cells can be made and frozen in large batches...

17. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    ...ChIP-Seq results. The FLAG epitope is quite small—20 amino acids—so we don’t think it interferes with the function...

18. Plasmids 101: Blue-white Screening

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    Blog Post

    ... enzyme. Providing DNA encoding this section of amino acids (called the α-peptide) to a lacZΔM15-mutant...

19. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    ... CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

20. 3 Challenges in Plant Synthetic Biology

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    ...Molecular Biophysics and Biochemistry from the Illinois Institute of Technology in 2004, she accepted ...