We narrowed to 319 results for: INO
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TypeBlog Post...lines. Lastly, the Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor, sufficiently suppresses basal...
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Summer SciComm Series: A PhD in Science Communication
TypeBlog Post...scientific methodology makes it easier to spot even minor weakness or limitations in a piece of research (... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post...University, who is majoring in marine biology and minoring in studio arts photography. References and resources... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...pMinDisplay vector. GPI contains a C-terminal glycosylphostidylinositol anchor. SGZ contains a PDGFR transmembrane... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog Post...professionals, joined the platform; while they are a minority, their help has been essential to push certain... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...just 1 minute of exposure. MG-2I-dL5** experienced minor bleaching after longer exposure times. Additionally... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog Post... enhanced “pdDronpa” domains could be placed in amino acid loops on either side of an enzyme’s active ... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog Post... can never completely eliminate the risk that a minor variation in experimental conditions will derail... -
Components of CRISPR/Cas9
TypeBlog Post...aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus sp. (AsCpf1) and Lachnospiraceae bacterium ... -
CRISPR Plasmids - Plants
TypeCollection... gRNA. Adenine base editors convert adenine to inosine, which is replaced by guanosine to create A->G ... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog Post...lab. Chemicals like calcium phosphate and diethylaminoehtyl (DEAE)-dextra neutralize or even impart an... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog Post.... 2017 Aug 24;170(5):956-972.e23 Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex... -
Antibodies 101: Isotypes
TypeBlog Post...have different versions or subclasses that have minor differences in their heavy chain constant domains... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post...presented study analysed a model of hepatocellular carcinoma, a cancer which can develop from a state of chronic... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...fluorescent proteins, differing by only a single amino acid. Without further information about properties... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog Post...The mixed PCR and incomplete PCR methods yield a minority of usable product with two 5′ overhangs, so these... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post... available tools such as the Raspberry Pi, the Arduino, or 3D printers are key to enabling this work. ... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post...phage recombination machinery is induced using L-arabinose. These cells can be made and frozen in large batches... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog Post...ChIP-Seq results. The FLAG epitope is quite small—20 amino acids—so we don’t think it interferes with the function... -
Plasmids 101: Blue-white Screening
TypeBlog Post... enzyme. Providing DNA encoding this section of amino acids (called the α-peptide) to a lacZΔM15-mutant...