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We narrowed to 525 results for: MEA

Showing: 201 - 220 of 525 results

  1. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...FLEx systems are generally considered "Cre-On" meaning that your gene of interest starts in the inverse...Resrouces on the Addgene Blog  Read about Other Means of Genome Engineering Using FLEx AAV Vectors? Learn...

  2. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...do it well" – even the smallest program. I don't mean have fancy food or a fantastic view (these are nice...Timing is everything Many of my suggestions are meant to keep the event on time. Nobody likes a program...

  3. Transferable Skills: Negotiation

    Type
    Blog Post
    ... sum game - that is, if I get what I want, that means you don’t. In this mindset, one of us wins, the ... negotiation is the most straightforward, it can mean that everyone leaves disappointed (we’ve all won...

  4. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...Specific binding of scFVs to cognate aaRS antigens as measured by suspension bead assay. Image from  Preger et...found a way to use the accumulation of indels to measure time. These plasmids with different promoters driving...

  5. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ... control assays for AAVs. These steps include measuring viral titer and assessing purity. But Addgene ...get hits to “random” genes. Do these hits always mean the sample is contaminated? No - what really matters...

  7. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...have died, usually after four to five days. This means that viral production takes a little bit longer ...Titers: Where Do They Come From and What Do They Mean? Overcoming the Challenges of Lentivirus Production...

  8. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...limit can feel like a lot of pressure. However, it means that your manager (and you) will be motivated to...time, you’ll know more people in industry, which means you’re more likely to have the right contacts and...

  9. Pooled Library Amplifications

    Type
    Blog Post
    ...aim for somewhere between 30x and 200x coverage, meaning that you need 30 to 200 times as many colonies ...recombination, we recommend ensuring the cells used are meant for repetitive sequences (such as STBL4 or Endura...

  10. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...suggest retransforming the plasmid into a strain meant for DNA purification. It sounds like extra work,...harvesting and miniprepping at various time points. Measuring the optical density of the culture can indicate...

  12. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...vectors do not have to exclusively be used as a means of detection; they are useful almost anytime you...Glycine and Proline residues found on the C-terminus meaning the upstream cistron will have a few additional...

  13. Science Guides

    Type
    Guide
    ...Optogenetics integrates optics and genetic engineering to measure and manipulate cells (frequently neurons) and their...optogenetics research utilize light to detect, measure, and control molecular signals and cells in order...

  14. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...Importantly, it is an immune privileged compartment, meaning that it can tolerate the presence of antigens (... size of its DNA cargo - 4.8 kb at most - which means that a gene larger than 3 kb cannot be delivered...

  15. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...going: If your PTA has a general meeting and a means to request funding for events, consider reaching...as coming from networking at the time, but that means it was the best kind of networking! Since nearly...

  16. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ... a stable, easily purified protein with easily measured activity, MDH is particularly amenable to in-class...tag, and compared their specific activities by measuring NADH production.   CUREs are beginning to...

  19. Hot Plasmids - August 2020

    Type
    Blog Post
    ... natural selection of sequences that evolve a measurable property of a protein. As a proof of concept,... allow for sensitive and high spatiotemporal measurements of norepinephrine. These norepinephrine sensors...

  20. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...complexes, wash away unbound protein, elute and measure the target. However, the two different approaches...the additional benefit of permanent linkage. This means that the primary antibody will not co-elute with...
Showing: 201 - 220 of 525 results