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We narrowed to 551 results for: NOL

Showing: 201 - 220 of 551 results

  1. March for Science

    Type
    Blog Post
    ...impact (15). Diversity and inclusion in science, technology, engineering and mathematics (STEM) (17). This... 27). In order to achieve the breakthroughs, technologies, and treatments promised by modern research,...Trump’s first budget Environmental Science and Technology: Crossing The Imaginary Line The New York Times...

  2. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...Multiplexing and Optogenetics across Scales. Trends Biotechnol 36, 1230-1243 (2018). Shcherbakova, D.M. et al...encoded calcium indicator for in vivo imaging. Nat Biotechnol 39, 368-377 (2021). Shcherbakova, D.M. Near-infrared... fluorescent protein for in vivo imaging. Nat Biotechnol 29, 757-761 (2011).   ...

  3. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...) either produced de novo using yeast display technology or produced from hybridoma antibodies developed...synthetic rAbs, developed using yeast display technology, are directed to integrins that bind the Arginine-Glycine-Aspartate...science, IPI hosts events and training courses on technologies and laboratory techniques related to antibody...

  4. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...avidin-biotin technology to improve cell adhesion on nanofibrous matrices.” Journal of Nanobiotechnology 13.1 ...interaction with avidin for molecular biology and biotechnology purposes.  Once a polypeptide is biotinylated...

  5. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...especially in in vivo models, and new Cre-lox based technologies are still being developed today. In this post...name Brainbow is truly fitting for this colorful technology. Optimizing the system: Brainbow-3 Although Brainbow... et al. released a refinement of the Brainbow technology, Brainbow-3.0, with the goal of overcoming the...

  7. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...recombination mediated by 28 bp direct repeats. Mol Biotechnol. 2008 Nov;40(3):252-60. doi: 10.1007/s12033-008... on efficacy, safety and upstream production. Biotechnol Adv. 2009 Jul-Aug;27(4):353-70. doi: 10.1016/...seed-stock and fed-batch fermentation processes. Biotechnol Bioeng. 2009 Aug 15;103(6):1129-43. doi: 10.1002...

  8. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ... Why might one be interested in state machine technology? Biological research has produced a massive amount...PBAD), and the A118 gene downstream of the diacetylphloroglucinol (DAPG)-inducible promoter (PPhlF).  Output...synthetic circuits from genetic context." Nature biotechnology 30.11 (2012): 1137-1142. PubMed PMID: 23034349...

  9. Important Considerations When Using AAVs

    Type
    Blog Post
    ...tubes, snap freeze in liquid nitrogen or a dry ice/ethanol bath, and store at -80° C. Once thawed, the AAV...purposes. The University of Pennsylvania and the biotechnology firm Biogen have recently collaborated to develop...is their ability to be used with new exciting technologies (such as CRISPR/Cas9) as they allow for gene...

  10. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    .... With the widespread adoption of CRISPR/Cas9 technology and rapid improvements in the field, it has recently...experiments (in collaboration with Integrated DNA Technologies, USA). These synthetic RNAs can also be purchased...delivery of gRNA, Cas9 nuclease, and donor DNA. J Biotechnol 241: 136-146. PubMed PMID: 27845164. 5. Clarke...

  11. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ..., which are pivotal in their application in biotechnology (Grimm & Lavis, 2022). These variations directly...toxicity due to the permeation process. Advanced technologies in fluorescent dyes/ligands Throughout the history...capabilities of fluorescence-based techniques in biotechnology. Recent examples of advancements include the...

  12. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ... variety of fields including neuroscience and immunology. They can be used for qualitative and quantitative...Historical Perspective and Future Outlook. Front Immunol 8: . https://doi.org/10.3389/fimmu.2017.01589 ..., IMMUNOGENS, VACCINES, AND IMMUNIZATION. In: Immunology Guidebook. Elsevier, pp 17–45. https://doi.org...

  13. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...the history of neuroscience research we have a technology that allows us to show causality in neural circuits...technique doesn’t have its limitations or that other technologies need to be discarded but clearly we are in an...and relatively cheap (compared to many other technologies and techniques in the sciences). But how exactly...

  14. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...honest — kits are quick, easy, and don’t involve phenol-chloroform.  The default option is to use the ...things like genomic DNA, RNA, proteins, salts, ethanol, or endotoxins. The most common method for checking...issues. For example, some kits reduce the risk of ethanol carry-over into the eluate, while other kits are...

  16. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...permeabilization protocol, but very poorly in a methanol fixation-permeabilization protocol. Although the...targets, we usually test 3 different fix/perms (methanol, PFA-saponin, PFA-triton). The fix/perm solutions....1% saponin; 4% PFA and 0.1% Triton X-100; and methanol, we found that the optimal perm/fix solution depended...

  17. CRISPR Guide Update

    Type
    Blog Post
    ...share and describe some incredible new CRISPR technologies developed and widely adopted in the past few...guide for a great introduction to what CRISPR technologies have to offer. Happy CRISPR-ing! More resources...

  18. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...nervous system With the advent of viral vector technology, new methods have been developed to study neuronal... Gradinaru Lab at the California Institute of Technology generated novel AAVs (Chan et al., 2017) that...targets neonatal neurons and adult astrocytes. Nat Biotechnol 27, 59–65. PubMed PMID: 19098898. PubMed Central...

  19. Technical Design of a Western Blot

    Type
    Blog Post
    ...are not with denaturing agents like DTT or β-mercaptoethanol.  Denaturing proteins To denature your proteins...you'll want to use SDS and either DTT or β-mercaptoethanol (BME). DTT is a stronger reducing agent than...without staining; simply wet your membrane with 20% methanol and place it on a light box after drying. Nitrocellulose...
Showing: 201 - 220 of 551 results