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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 423 results for: NUC

Showing: 201 - 220 of 423 results

  1. CRISPR Plasmids - Tagging

    Type
    Collection
    ... tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, fibrillarin (FBL). To repeat the knock-in...Cas to generate conditional alleles of essential nuclear and cytoplasmic proteins, which can then be depleted...articles: Kunzelmann, et al. G3 2016 Bottcher, et al. Nucleic Acids Res. 2014 The Förstemann lab has provided...

  2. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ... "WDDD: worm developmental dynamics database." Nucleic acids research 41.D1 (2012): D732-D737. PubMed ... a platform for 3′ UTR biology in C. elegans." Nucleic acids research 36.suppl_1 (2007): D57-D62. PubMed...

  5. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...Assembly: a) Graphic depiction of chromatin within a nucleus -> DNA compaction around histones -> Examples of...libraries encode target sites flanked by randomized nucleotides in place of a protospacer adjacent motif (PAM...

  6. Hot Plasmids - October 2020

    Type
    Blog Post
    ... than the more efficient single-stranded oligodeoxynucleotide donors (ssODN). So how can you use CRISPR...codon-optimized, engineered Streptococcus canis Cas9 nuclease that has a broad 5′-NNG-3′ PAM. Get the plasmids...

  9. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...administration. However, until recently safe delivery of nucleic acid cargo (DNA or RNA) to target cells has been...Self-complementary AAV mediates gene targeting and enhances endonuclease delivery for double-strand break repair." Gene...

  10. A Look at Addgene's QC Process

    Type
    Blog Post
    ...mutations, frame shifts, missing elements, and nucleotide indels or mismatches in significant plasmid elements... reference; and identifying mixed plasmid and nucleotide populations. All of these topics require additional...

  11. Hot Plasmids February 2024

    Type
    Blog Post
    ...primary cells. Although cell entry of a Cas9 ribonucleoprotein (RNP) was limited (likely due to its long,...Cell-penetrating Cas9, fused to HIV TAT, Myc and SV40 Nuclear Localization Signals, and GFP. B) Workflow to assess...

  13. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...protected by histone-like proteins forming the nucleocapsid. B) Three generations of adenoviral vectors:...purified plasmid stock should be analyzed by endonuclease restriction digest or fully sequenced to confirm...

  15. CRISPR References and Information

    Type
    Collection
    ...outcomes from cleaving nucleases (e.g. Cas9, Cas12a) and noncleaving nucleases (e.g. base editing). Developed...an accession number, genomic location, pasted nucleotide sequence, or a sequence text file you upload....

  16. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...including mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs ...power of this system lies in the ability of the endonuclease Cas9 to create a DNA double stranded break (...

  17. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...constructs for visualizing cellular components (nucleus, endosomes, lysosome, golgi, ER, inhibitory synapses...TnpB (A) and Cas9 (B) genome editors. Effector nucleases are shown bound to the target DNA, recruited by...

  20. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...endA+. endA encodes a thermostable periplasmic endonuclease that can sometimes co-purify with your plasmid...recommend performing an extra wash step to remove endonucleases (see figure at right for an example). Look out...
Showing: 201 - 220 of 423 results