We narrowed to 422 results for: NUMB

...necessarily publish in OA journals themselves. A good number of those surveyed (66 percent) gave preference ...

...phages will test. These includes labs with a large number of different phages or with phages targeting organisms...

...determining the subcellular localization and the number of disulfide bonds in your protein - two parameters...

...mEGFP-pBAD for bacterial expression, as well as a number of fusions for cellular localization studies including...

...garner much international attention. Their small numbers do not, however, make these patients suffer any...

...males and females are to parity - the smaller the number, the more it leans towards males). This ratio is...

...some biological systems one “might need larger numbers.” Further details and protocols are available online...

...100 x 100 x 100 ÷ 0.1 This number should be at least 1000X greater than number of perturbations in library...plasmids and can be scaled according to the actual number. Required Quality Control (QC) Pooled libraries...individual plasmids. For larger libraries scale the number of cells and DNA accordingly. Pro-Tip Use of extreme...order to be enumerated accurately. Frequently the number of colonies can be too great to count. Ideally,...a given plate (example: by increasing the total number of bioassay plates used). Scaling up the experiment...Maxiprep protocol, scale the reagent volume and column number as needed. The use of Mega or Gigapreps is acceptable...acceptable when scaling up or reducing the number of tandem purifications. Consult the manufacturers handbooks...

...for the Addgene blog. However, it has a limited number of drawing tools, so it’s harder to draw intricate...

...Nanobody" name.  We’ve talked about sdAbs a number of times on this blog over the years and have described...

... DNA tool: Removes non-DNA characters, such as numbers and blank spaces, from the text of a DNA sequence...

...with the citation and your deposit or plasmid ID numbers and we’ll be happy to update the plasmids to add...

...recombination of the gene construct. A predictable number of cells will express the effector or retain the...

...replication (which controls the host range and copy number of the plasmid) and typically include a gene that...

...it is technically challenging to collect large numbers of cells or when a high degree of signal to noise...

...Cas9 is the genome editing tool of choice for a number of model organisms: mammalian cells, yeast, drosophila...

...then by increasing the consensus between a greater number of extinct transposon sequences and then testing...

...visitors to the poster were impressed to see how the numbers of plasmid genome engineering tools – both deposited...

...scientist-in-training will spend 10 or more years with a small number of formal advisors learning how to be a scientist...

...present in large numbers, 50 or more per cell, and have a high copy number. High copy number plasmids should...copy and low copy plasmids? The copy number refers to the number of copies of an individual plasmid within... of bacteria and is used to grow up sufficient numbers of bacteria necessary to isolate enough plasmid...Figure 2). Large plasmids usually have a low copy number (approximately one or two copies per cell) and ...