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Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Type

Blog Post

...and their endogenous counterparts using western blotting. The subcellular localization of BiFC constructs...
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

Type

Blog Post

...easier with a direct link to the plasmid page at the bottom of the dropdown list: The search bar will suggest...
An Introduction to Adenovirus

Type

Blog Post

.... Rowe, W. P., Huebner, R. J., Gilmore, L. K., Parrott, R. H., & Ward, T. G. (1953). Isolation of a Cytopathogenic...
Transferable Skills Guide: Identifying Your Transferable Skills

Type

Blog Post

... given yourself the bathroom mirror pep talk, forgotten and then retrieved your lucky socks, persevered...
Negative Can Be Positive: Open AAV Data with Addgene

Type

Blog Post

... A. M., Su, L., Su, Q., Gao, G., Mueller, C., & Flotte, T. R. (2015). Stability and compatibility of recombinant...
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

Type

Blog Post

...protein instead of three Cas proteins (Fig. 1A, bottom), and require only two transposase proteins (TnsB...
Tips from the Repository Trenches: Using Barcodes to Track Samples

Type

Blog Post

...something is removed from this tube, the barcode on the bottom is scanned to verify that it is the correct sample...
A Career in Grants: How To Become a Grant Professional

Type

Blog Post

...competencies through a multiple choice exam. The bottom line on being a grants professional The Grants ...
Negotiating Work and Life: How to Find the Joy

Type

Blog Post

...time with your friends – no spouses or kids. The bottom line We don’t just “do science” – we are scientists...
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

Type

Blog Post

...the LY6A receptor to gain access to the CNS. The bottom line of viral vector permissiveness While PHP.B...
New Tools Enable CRISPRa for Neuroscience Applications

Type

Blog Post

...top) and neuron-specific expression of dCas9-VPR (bottom). Created with BioRender.com. The Day Lab used...
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

Type

Blog Post

... by producing a detectable signal in urine. The bottom line with plasmid addiction systems While selfish...
The PAM Requirement and Expanding CRISPR Beyond SpCas9

Type

Blog Post

...doi.org/10.1038/nature26155 Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O,...
Finding nucleic acids with SHERLOCK and DETECTR

Type

Blog Post

...access to reagents and equipment has become a bottleneck. In the last few years, scientists have utilized...
New CRISPR Tools: Cas7-11 and PASTE

Type

Blog Post

...whether the samples are from a rain gutter or the bottom of Tokyo Bay. When a weird, sulfurous smelling,...
Hot Plasmids - November 2023

Type

Blog Post

...fact, Anti-PSD-95 [K28/43R] has been one of the hottest antibodies in Addgene’s collection since we began...
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

Type

Blog Post

...-MB-231 cell lysate after 4-days of expression (bottom, scalebar = 0.5µm). (d) Ultrasound imaging of MDA-MB...
CRISPR Activation: A Practical Guide

Type

Blog Post

...Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J,...
The Challenges of Cell Culture

Type

Blog Post

...public databases. Image attributions (from top to bottom) Database Center for Life Science (DBCLS), National...
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Type

Blog Post

... Disease & Open Science: Interview with Darrell Kotton Four Factors that Differentiate the Stem Cell Field...

Showing: 201 - 220 of 286 results

Results per page:

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

An Introduction to Adenovirus

Transferable Skills Guide: Identifying Your Transferable Skills

Negative Can Be Positive: Open AAV Data with Addgene

INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

Tips from the Repository Trenches: Using Barcodes to Track Samples

A Career in Grants: How To Become a Grant Professional

Negotiating Work and Life: How to Find the Joy

Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

New Tools Enable CRISPRa for Neuroscience Applications

Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

The PAM Requirement and Expanding CRISPR Beyond SpCas9

Finding nucleic acids with SHERLOCK and DETECTR

New CRISPR Tools: Cas7-11 and PASTE

Hot Plasmids - November 2023

New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

CRISPR Activation: A Practical Guide

The Challenges of Cell Culture

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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