We narrowed to 290 results for: Ott;

1. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   ...and their endogenous counterparts using western blotting. The subcellular localization of BiFC constructs...

2. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

   Type

   Blog Post

   ...easier with a direct link to the plasmid page at the bottom of the dropdown list: The search bar will suggest...

3. Isolating Genomic Regions of Interest with the CRISPR System

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   Blog Post

   ... of many human malignancies. Additionally, the Tapscott Lab identified regulators of DUX4, a transcriptional...

4. Simplify Cloning with in vivo Assembly

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   Blog Post

   ...biology project, yet very often, it is also a bottleneck in the experimental process. The majority of ...

5. Negative Can Be Positive: Open AAV Data with Addgene

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   Blog Post

   ... A. M., Su, L., Su, Q., Gao, G., Mueller, C., & Flotte, T. R. (2015). Stability and compatibility of recombinant...

6. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   Blog Post

   ...protein instead of three Cas proteins (Fig. 1A, bottom), and require only two transposase proteins (TnsB...

7. Tips from the Repository Trenches: Using Barcodes to Track Samples

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   Blog Post

   ...something is removed from this tube, the barcode on the bottom is scanned to verify that it is the correct sample...

8. A Career in Grants: How To Become a Grant Professional

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   Blog Post

   ...competencies through a multiple choice exam.  The bottom line on being a grants professional The Grants ...

9. Negotiating Work and Life: How to Find the Joy

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   Blog Post

   ...time with your friends – no spouses or kids. The bottom line We don’t just “do science” – we are scientists...

10. Chemogenetics Guide

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    Guide

    ...Slaviero AN, Silvagnoli AD, Crespo EL, Schalau R, Gott M, Tree MO, Dunbar GL, Rossignol J, Hochgeschwender...

11. Hot Plasmids - November 2023

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    Blog Post

    ...fact, Anti-PSD-95 [K28/43R] has been one of the hottest antibodies in Addgene’s collection since we began...

12. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    Blog Post

    ...-MB-231 cell lysate after 4-days of expression (bottom, scalebar = 0.5µm). (d) Ultrasound imaging of MDA-MB...

13. How to Write a Scientific Review Article

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    Blog Post

    ...overthink it. And to quote my adviser, "you just gotta do it." At the end of the day, someone has to write...

14. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    Blog Post

    ... Disease & Open Science: Interview with Darrell Kotton Four Factors that Differentiate the Stem Cell Field...

15. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ...doi.org/10.1038/nature26155 Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O,...

16. Finding nucleic acids with SHERLOCK and DETECTR

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    Blog Post

    ...access to reagents and equipment has become a bottleneck. In the last few years, scientists have utilized...

17. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

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    Blog Post

    ...the LY6A receptor to gain access to the CNS. The bottom line of viral vector permissiveness While PHP.B...

18. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post

    ...top) and neuron-specific expression of dCas9-VPR (bottom). Created with BioRender.com. The Day Lab used...

19. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post

    ... by producing a detectable signal in urine. The bottom line with plasmid addiction systems While selfish...

20. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post

    ...whether the samples are from a rain gutter or the bottom of Tokyo Bay. When a weird, sulfurous smelling,...