We narrowed to 443 results for: RELA

...SpCas9 (2). In many cases this is due both to lower relative nuclease efficiencies as well as to more stringent...tolerance and, consequently, increased specificity relative to the more commonly employed 20nt gRNAs. It should...

...surrounded by lab members who are all working on related topics, scientific discussion is often part of ...resources on the Addgene blog Check out all careers related blog posts here Find 9 tips for a successful postdoctoral...

...good, high quality preps due to its endA1 recA1 relA1 genotype, as these mutations improve plasmid stability...these factors are generally, but not perfectly, correlated. Typical growth times for high copy plasmids...

...in mind! This section of our plasmid pages is relatively new, so some of our older plasmids may not have...here. Articles Citing this Plasmid: This is a relatively new feature of our plasmid pages, and provides...

...major advances in light microscopy, particularly related to the neurosciences.  These developments in microscopy... our understanding of the structure-function relationship of the central nervous system under normal and...

...and bacterial transformation. And more plasmid-related educational resources can be found in our Plasmids...the repository. These protocols show up on the related plasmid pages and can help scientists use these...

...plasmid is packaged for viral delivery, or the relative expression levels between two or more genes is... plasmids listed in the table above (and their related plasmids) are designed to have one or more of the...

...not narrow enough to distinguish between closely related colors. Furthermore, most FPs  have a broad range...College of Medicine. He is interested in everything related to gene expression, particularly at the RNA level...

...necessary because split FPs can associate, albeit at relatively low frequencies, without being brought into close...visualized makes it amenable to studying PPIs under relatively physiological conditions when compared to FRET...

...before then. Other areas are newer, such as the relatively recent resurgence of “Do-It-Yourself Science”...hadn’t made manipulating the genome cheap and relatively easy? Have all of these things contributed to...

...particular scientific subfields.  Plasmid requests correlate with paper publication The dataset includes 11,426...and uncertainty in estimating parameters from relatively sparse data from China. An important additional...

...polymerase chain reaction Browse our blog posts related to viral vector protocols and tips Read all of ... by qPCR Browse our collection of viral vector related protocols Learn more about the Addgene viral service...

...rather “let’s see how we can make this fun activity relate to STEM.” Figure 1 shows the activities we had ...molecular mechanism, I was careful to keep it relatively simple. I honed the complexity of the talk by...

... some people still use amp? Carb is expensive (relative to amp), and for some applications, the benefit...their mode of action and have a very similar relationship as amp and carb (they are interchangeable). ...

...die or differentiate Step 3 reveals which NANOG-related genes are critical to stem cell survival. Knowing... use next-generation sequencing to measure the relative proportion of guide RNAs (gRNAs) across the population...

...reliably distinguish between Zika and a closely-related virus, Dengue from multiple sample sources. SHERLOCK...from cell-free DNA fragments as well as health-related single nucleotide polymorphisms (SNPs) from human...

...this information to identify a large variety of related bacteria with similar Cas systems. Some of these...knockdown efficiencies. The ability to identify related Cas systems opens up the potential of further development...

...session is to start with something completely unrelated to the issue and consider how it could be shaped...problem is to step away from it. Find something unrelated to do (many Addgenies enjoy rock climbing, for...

...editing efficiency based on the location of the ‘A’ relative to the protospacer adjacent motif (PAM). ABE7.10...edit in a stretched window ranging from 1-17 bp relative to the PAM sequence (Zong et al., 2018) (see CBE...

... protein fluorophores and quenchers are still relatively bulky. One of the advantages of oligonucleotides...hydroxybenzylidene imidazolinone (DFHBI) with relatively low fluorescence in vivo, the Jaffrey lab used...