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  1. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...good, high quality preps due to its endA1 recA1 relA1 genotype, as these mutations improve plasmid stability...these factors are generally, but not perfectly, correlated. Typical growth times for high copy plasmids...

  2. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...in mind! This section of our plasmid pages is relatively new, so some of our older plasmids may not have...here. Articles Citing this Plasmid: This is a relatively new feature of our plasmid pages, and provides...

  3. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...surrounded by lab members who are all working on related topics, scientific discussion is often part of ...resources on the Addgene blog Check out all careers related blog posts here Find 9 tips for a successful postdoctoral...

  4. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...SpCas9 (2). In many cases this is due both to lower relative nuclease efficiencies as well as to more stringent...tolerance and, consequently, increased specificity relative to the more commonly employed 20nt gRNAs. It should...

  5. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...major advances in light microscopy, particularly related to the neurosciences.  These developments in microscopy... our understanding of the structure-function relationship of the central nervous system under normal and...

  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...plasmid is packaged for viral delivery, or the relative expression levels between two or more genes is... plasmids listed in the table above (and their related plasmids) are designed to have one or more of the...

  7. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...not narrow enough to distinguish between closely related colors. Furthermore, most FPs  have a broad range...College of Medicine. He is interested in everything related to gene expression, particularly at the RNA level...

  8. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ... some people still use amp? Carb is expensive (relative to amp), and for some applications, the benefit...their mode of action and have a very similar relationship as amp and carb (they are interchangeable). ...

  13. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...polymerase chain reaction Browse our blog posts related to viral vector protocols and tips Read all of ... by qPCR Browse our collection of viral vector related protocols Learn more about the Addgene viral service...

  14. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...rather “let’s see how we can make this fun activity relate to STEM.” Figure 1 shows the activities we had ...molecular mechanism, I was careful to keep it relatively simple. I honed the complexity of the talk by...

  15. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...reliably distinguish between Zika and a closely-related virus, Dengue from multiple sample sources. SHERLOCK...from cell-free DNA fragments as well as health-related single nucleotide polymorphisms (SNPs) from human...

  16. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...editing efficiency based on the location of the ‘A’ relative to the protospacer adjacent motif (PAM). ABE7.10...edit in a stretched window ranging from 1-17 bp relative to the PAM sequence (Zong et al., 2018) (see CBE...

  17. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ... protein fluorophores and quenchers are still relatively bulky. One of the advantages of oligonucleotides...hydroxybenzylidene imidazolinone (DFHBI) with relatively low fluorescence in vivo, the Jaffrey lab used...

  19. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...population. Heat maps are often used to indicate the relative density of cells. You can see both of these features...intracellular IFNγ, IL-17 and IL-10 producing T cells correlates with the occurrence of post-transplant opportunistic...

  20. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...this information to identify a large variety of related bacteria with similar Cas systems. Some of these...knockdown efficiencies. The ability to identify related Cas systems opens up the potential of further development...
Showing: 201 - 220 of 436 results