We narrowed to 443 results for: RELA
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  TypeBlog Post...surrounded by lab members who are all working on related topics, scientific discussion is often part of ...resources on the Addgene blog Check out all careers related blog posts here Find 9 tips for a successful postdoctoral...
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  Truncated gRNAs for Regulating Gene ExpressionTypeBlog Post...SpCas9 (2). In many cases this is due both to lower relative nuclease efficiencies as well as to more stringent...tolerance and, consequently, increased specificity relative to the more commonly employed 20nt gRNAs. It should...
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  Plasmids 101: Optimizing Plasmid YieldsTypeBlog Post...good, high quality preps due to its endA1 recA1 relA1 genotype, as these mutations improve plasmid stability...these factors are generally, but not perfectly, correlated. Typical growth times for high copy plasmids...
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  Anatomy of a Plasmid Page at AddgeneTypeBlog Post...in mind! This section of our plasmid pages is relatively new, so some of our older plasmids may not have...here. Articles Citing this Plasmid: This is a relatively new feature of our plasmid pages, and provides...
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  Light Sheet Fluorescence MicroscopyTypeBlog Post...major advances in light microscopy, particularly related to the neurosciences. These developments in microscopy... our understanding of the structure-function relationship of the central nervous system under normal and...
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  Sharing Your Lab Protocols: Using Apps to Save Time & Track Your ExperimentsTypeBlog Post...and bacterial transformation. And more plasmid-related educational resources can be found in our Plasmids...the repository. These protocols show up on the related plasmid pages and can help scientists use these...
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  Plasmids 101: Multicistronic VectorsTypeBlog Post...plasmid is packaged for viral delivery, or the relative expression levels between two or more genes is... plasmids listed in the table above (and their related plasmids) are designed to have one or more of the...
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  Which Fluorescent Protein Should I Use?TypeBlog Post...not narrow enough to distinguish between closely related colors. Furthermore, most FPs have a broad range...College of Medicine. He is interested in everything related to gene expression, particularly at the RNA level...
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  SciComm: Taking Science to Elementary and Middle SchoolsTypeBlog Post...rather “let’s see how we can make this fun activity relate to STEM.” Figure 1 shows the activities we had ...molecular mechanism, I was careful to keep it relatively simple. I honed the complexity of the talk by...
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  Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions EasyTypeBlog Post...necessary because split FPs can associate, albeit at relatively low frequencies, without being brought into close...visualized makes it amenable to studying PPIs under relatively physiological conditions when compared to FRET...
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  Capturing a Moment with Marc Zimmer’s “The State of Science”TypeBlog Post...before then. Other areas are newer, such as the relatively recent resurgence of “Do-It-Yourself Science”...hadn’t made manipulating the genome cheap and relatively easy? Have all of these things contributed to...
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  The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene DataTypeBlog Post...particular scientific subfields. Plasmid requests correlate with paper publication The dataset includes 11,426...and uncertainty in estimating parameters from relatively sparse data from China. An important additional...
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  Plasmids 101: Choosing an Antibiotic Resistance GeneTypeBlog Post... some people still use amp? Carb is expensive (relative to amp), and for some applications, the benefit...their mode of action and have a very similar relationship as amp and carb (they are interchangeable). ...
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  Droplet Digital PCR for AAV QuantitationTypeBlog Post...polymerase chain reaction Browse our blog posts related to viral vector protocols and tips Read all of ... by qPCR Browse our collection of viral vector related protocols Learn more about the Addgene viral service...
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  CRISPR Between the Genes: How to Experiment with Enhancers and EpigenomicsTypeBlog Post...die or differentiate Step 3 reveals which NANOG-related genes are critical to stem cell survival. Knowing... use next-generation sequencing to measure the relative proportion of guide RNAs (gRNAs) across the population...
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  CRISPR-mediated Plant Base EditorsTypeBlog Post...editing efficiency based on the location of the ‘A’ relative to the protospacer adjacent motif (PAM). ABE7.10...edit in a stretched window ranging from 1-17 bp relative to the PAM sequence (Zong et al., 2018) (see CBE...
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  Fluorescent Proteins 101: Aptamer FluorophoresTypeBlog Post... protein fluorophores and quenchers are still relatively bulky. One of the advantages of oligonucleotides...hydroxybenzylidene imidazolinone (DFHBI) with relatively low fluorescence in vivo, the Jaffrey lab used...
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  New CRISPR Tools: Cas7-11 and PASTETypeBlog Post...this information to identify a large variety of related bacteria with similar Cas systems. Some of these...knockdown efficiencies. The ability to identify related Cas systems opens up the potential of further development...
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  Transferable Skills Guide: CreativityTypeBlog Post...session is to start with something completely unrelated to the issue and consider how it could be shaped...problem is to step away from it. Find something unrelated to do (many Addgenies enjoy rock climbing, for...
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  Antibodies 101: Reading a Flow PlotTypeBlog Post...population. Heat maps are often used to indicate the relative density of cells. You can see both of these features...intracellular IFNγ, IL-17 and IL-10 producing T cells correlates with the occurrence of post-transplant opportunistic...