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Showing: 201 - 220 of 601 results
  1. CRISPR Plasmids - Repress Gene Expression

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    Collection
    ... Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to top Plant...Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top...
  2. The Strength of Story Telling

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    Blog Post
    ...be the first author. When I raised this, he was pretty angry and started arguing with me. First I calmed...jumping backwards in time to explain something, rethink your structure. Develop your protagonist In most...
  3. Antibodies 101: Chimeric Antibodies

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    Blog Post
    ...In most cases, the resulting chimeric antibody retains the binding specificity to the original target ...In addition, chimeric antibodies will inevitably retain some sequence from their parental species which...
  4. Single-cell tracking of lineage and identity with CellTag

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    Blog Post
    ...cell types. MEFs were reprogrammed into iEPs by retroviral expression of transcription factors Foxa1 and.... Adding Mettl7a1 to the standard Foxa1-Hnf4α retroviral overexpression cocktail yielded a three-fold ...
  5. AAV for Neuronal Tracing

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    Collection
    ...viral service encoding tools for monosynpatic retrograde neuronal tracing, to be used with modified rabies...be used with modified rabies virus to perform retrograde neuronal tracing. Note : Addgene does not distribute...deletion-mutant rabies virus AAV1 Ian Wickersham 100799 pAAV-TREtight-mTagBFP2-B19G AAV1 Ian Wickersham Introduction..., Finke S, Conzelmann KK, Callaway EM. 2007a. Retrograde neuronal tracing with a deletion-mutant rabies...
  6. The Importance of High Titer for AAV Transductions

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    Blog Post
    ...therefore higher titer will be required. For example, retrograde AAV gets transported up the cell’s axon. While...even larger area, thus necessitating even higher retrograde AAV titers for successful transduction. In another...
  7. The MTA at Addgene

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    Blog Post
    .... Over the last twenty years, we’ve developed a pretty smooth system for this process. We often get positive... sent the MTA to sign. Once they've signed and returned the MTA, Addgene can move forward with shipping...
  8. Plasmids 101: NGS Quality Control for Pooled Libraries

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    Blog Post
    ...sequencing is successful, your NGS provider should return results in the form of many, many short DNA sequences...specific libraries, but any analysis script should return the same output: a spreadsheet of read counts. ...
  9. Improved Plasmid Maps Powered by SnapGene

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    Blog Post
    ... and sequence displays are now much easier to interpret and analyze at a glance. For a quick look at just...“Analyze Sequence” button. The maps hosted here retain all the features shown in the static maps discussed...
  10. Flow Cytometry Readouts: Yes, No, and Everything in Between

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    Blog Post
    ...plot. In this post, I’ll walk you through how to interpret the antibody readout on a flow plot.  Note that...characterize your samples may allow for better interpretation. Priyamvada Prathima has a research background...
  11. Behind the scenes: Addgene’s new search engine and more

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    Blog Post
    ...website resources that best match a user’s query, and return the matching resources in an order that makes sense...expose as filters and designed the search engine to return only the filters that are relevant to a specific...
  12. Viral Vectors 101: Viral Applications

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    Blog Post
    ...one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided on an application...attenuated, nef-deleted HIV-1 strains in vivo. Retrovirology. 2007 Sep 23;4:66. doi: 10.1186/1742-4690-4-...
  13. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...envelope protein for producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids...as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates...
  14. How Addgene Creates Content

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    Blog Post
    ...quickly." Final design (right). Immediate and immense regret of the graphic designer not shown.   However... and knowledge changes and require updating or retiring. CRISPR/Cas9, for example, has gone through rapid...
Showing: 201 - 220 of 601 results