Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 583 results for: Ret;

Showing: 201 - 220 of 583 results

  1. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...high-throughput splinkerette-PCR method for the isolation and sequencing of retroviral insertion sites...during the differentiation process. Moreover, a splinkerette-PCR assay may be used to map the specific genetic...

  2. Finding Your Perfect Job After University

    Type
    Blog Post
    ...experience. Working in industry: Microbiology On returning from Ghana at the beginning of 2010, I explored...looking for other career options after 6 months. Returning to university to study a Masters of Science I ...programming. Working for a scientific nonprofit On returning to the UK, I checked out the job postings from...

  3. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    .... Pro-Tips: 1. It’s all about the MOI. In retrospect I used a way too high Cas9 MOI considering that...I didn’t do this, but to do it you would need a pretty good gel image. If you are analyzing short DNA ...cancer cell line in about 1 month, which I’d say is pretty amazing. Despite the shifting landscape of research...

  4. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ... was not present (Kuhlman, SK. 2008). While theoretically this strategy should work, these vectors suffered...data and can instill greater confidence when interpreting the outcome.    References and resources References...Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH (2018) An Alternate Route for...

  5. Typing CRISPR Systems

    Type
    Blog Post
    ...gku241 Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W.,...., Terns, M. P., White, M. F., Yakunin, A. F., Garrett, R. A., Van Der Oost, J., . . . Koonin, E. V. (...Pinilla-Redondo, R., Mayo-Muñoz, D., Russel, J., Garrett, R. A., Randau, L., Sørensen, S. J., & Shah, S....

  6. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...Off-target activities of the RNAi Correctly interpreting your data therefore necessitates careful design..., this can make the results more difficult to interpret, and a knockout/ or knockdown approach should ...validation is critical to ensuring accurate interpretation of your results. Therefore, taking advantage...

  12. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...toward a more sustainable one. The math on this is pretty simple: wash your tips once and you cut your tips...distribute it to industrial and residential markets. Rethinking our approach to lab waste is something we all...

  13. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...EBFP-Cre Available in serotypes AAV1, AAV5, and AAV retrograde (the most popular form), this viral vector is...with Flp-FRT. Additionally, you could use the retrograde form to specifically express Cre in neurons with...

  15. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...foundation of multiple important technologies, including FRET and optogenetics. Even though GFP has been so thoroughly...characterized, it turns out this protein has a few more secrets - during a collaboration, members of Maureen Hanson...

  16. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...subtilis and the yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful ...peptide (SP) that routes the protein through the secretory pathway to improve folding and disulfide bond ...

  17. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    ...New Hampshire in the summer for the annual lab retreat. How do you manage work-life balance? Tom Ellis... “three generations walk[ing] together in the Arboretum and other parks.” Lab life is not all work and...

  18. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...for packaging: AAV1, AAV2, AAV5, AAV8, or AAV Retrograde (AAVrg) capsids.  Please be aware that this ordering...plasmids packaged in AAV1, AAV5, AAV8, and AAV Retrograde to be 1x1013 and titers for AAV2 to be 7x1012...

  20. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...ahold of your destinies” and also suggested some concrete actions that can be taken now to solve some of...Disequilibrium, Disillusion and The Postdoc Dilemma” which pretty much said it all.  She cited the oft quoted statistic...
Showing: 201 - 220 of 583 results