We narrowed to 221 results for: Rras

1. Viral Vectors 101: Optogenetic Tools

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   Blog Post

   .../doi.org/10.1073/pnas.1936192100 Tichy, A.-M., Gerrard, E. J., Sexton, P. M., & Janovjak, H. (2019). Light-activated...

2. Antibodies 101: Multiplex Immunofluorescence

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   ...Dumitras, C., Gong, B., Nguyen, K., Van Der List, D., Murray, K. D., Engebrecht, J., & Trimmer, J. S. (2019)...

3. Technique: Probe Phage Genomes for Host Binding Proteins

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   ...firmly onto each to remove cell debris. Then, UV-irradiate the membranes for 15 min to kill any remaining...

4. Generating Mouse Models Using CRISPR/Cas9

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   ..., Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering...

5. PiggyBac-ing Through the Genome Editing Field

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   ...s chromatin structure undergoes significant rearrangement (such as when a stem cell initiates differentiation...

6. Finding Your Perfect Job After University

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   ...project to validate some genomic changes, so this arrangement was ideal for everyone.  The cutting edge science...

7. Building Global Connections with the International Mentorship Program USA-EUROPE

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   ...University Miguel de Cervantes, the University of Navarra, and the University of the Basque Country. Mentees...

8. Luciferase Plasmid Collection

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   ...promoter is present for normalization Alejandro Ferrando 114670 pGL3-TK-5UTR-BsmBI-Luciferase Firefly Insertion...

9. Important Considerations When Using AAVs

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   ...therapy 8.16 (2001): 1248. PubMed PMID: 11509958. 8. Ferrari, Forrest K., et al. "Second-strand synthesis is...

10. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    ...for error-free knock-in at both alleles may be warranted (20-30% of all tagged clones were bi-allelic in...

11. Plasmids 101: Modular Cloning Applications and Kits

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    ...perfect combination. The EcoFlex Kit has a wide array of DNA parts that can be assembled into individual...

12. Custom CRISPR Screens & the Green Listed Software

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    Blog Post

    ...the Output_Short file directly. We have used CustomArray, Inc for this purpose.  The gRNA oligos are...

13. Special Delivery: Fluorophore Targeting for FRET Studies

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    ...100 Å). Researchers have developed an extensive array of chemical labeling strategies suitable for targeting...

14. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    ...to iPSCs and re-establishment after Partial rearrangement from one cell type to another Time and resources...

15. A Needle in a Base-Stack: Cas9 Structural Biology

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    ...Cas9 grips nucleotides 1-10 of the guide RNA, prearranged for base pairing with a complementary DNA strand...

16. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

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    ...academia as well as the non-profit sector are transferrable to industry such as technology transfer, communications...

17. Antibody Validation for Flow Cytometry

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    ... Bolivar, S. G., Ruiz Moleon, V., Eckmann, P., Worrall, D., McDowell, I., Southern, K., Reintsch, W., ...

18. Optogenetics + CRISPR, Using Light to Control Genome Editing

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    ... K866PCK mutant was indeed inactive prior to irradiation with UV light (365nm) and that post-UV exposure...

19. Validated gRNA Sequences

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    Collection

    ...AAAAAACCGAACTCCGCGCTGCGTAAAGTA 44505 cut S. pyogenes 23360965 Marraffini scramble synthetic AACCCCTGATTGTATCCGCA 62285...

20. CRISPR 101: Cytosine and Adenine Base Editors

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    ... A., Gehrke, J. M., Lee, S.-J., Liquori, A. J., Murray, R., Packer, M. S., Rinaldi, C., Slaymaker, I. ...