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We narrowed to 622 results for: SET

Showing: 201 - 220 of 622 results
  1. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    ...extracted, sectioned, and imaged, with all image sets available at the Allen Institute’s Genetic Tools...Enhancer Collection is a quick guide to a smaller set of tried-and-true winners, containing enhancers with...; font-weight: normal; text-decoration: none","font_set":"DEFAULT"},"button_hover_color":{"color":"#0052a9...
  2. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...Goedhart and colleagues in the Gadella Lab devised a set of optogenetic switches for the GEFs ITSN1, TIAM1...controlled phospholipase D (PLD). Now they present a set of variants called SuperPLDs with 10, 30, 50, and...
  3. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ... go over the details step-by-step. 1. Digestion Set up restriction digests for your insert (or donor ...of recipient plasmid to insert. It is critical to set up a negative control ligation reaction with no insert...
  4. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...approaches to visualize bacteria in an educational setting. The Symposium on ‘Creative Approaches to Presenting...
  5. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...the recipient plasmid, it is useful to see if one set of enzymes will work in the same restriction enzyme...future steps. Experimental Procedure Digest your DNA Set up restriction digests for your donor and recipient...recipient plasmid to insert. It is also important to set up negative controls in parallel. For instance, a...
  6. Editor's Choice, August 2016

    Type
    Blog Post
    ... part of my job, I travel around the country and set up meetings with PIs to discuss the benefits of depositing...
  7. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... produce a BRET probe for imaging. These authors set out to design a better fluorescent protein that could...could be excited by NanoLuc® luciferase (NLuc) and set about to create an in vivo imaging reporter by linking...impractical or infeasible for certain experimental setups. The brightness of NLuc has caught the attention...
  8. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...laboratories remains limited. Members of the Stanley Qi Lab set out to address this limitation by developing CRISPRkit...inexpensive system for CRISPR experiments in classroom settings without specialized equipment (Collins & Lau et... et al. (2025) under CC BY license. These datasets (in healthy mice and a Huntington's disease model...
  9. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...fusion protein allows for LSD1 targeting to a wider set of genomic targets. These two vectors are part of...may make sense to try multiple CRISPR tools when setting up your experimental system. Let us know about ...
  10. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...researchers are eager to apply it in a clinical setting. CRISPR is often used for animal germline modification...to deliver via gene therapy, so researchers have set their sights on another approach. Since shorter forms...
Showing: 201 - 220 of 622 results