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We narrowed to 556 results for: SPR

Showing: 201 - 220 of 556 results

  1. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...PubMed. More CRISPR-Cas9 Resources: CRISPR 101: a blog series on the basics of CRISPR-Cas technology... hazards of creating “gene drives” capable of spreading through wild populations. Whereas most genomic...inheritance in their favor and consequently can spread even when costly. If even a single organism carrying...escape the laboratory, the drive could eventually spread through the entire wild population with unpredictable...into a homozygote and ensuring that all future offspring inherit the endonuclease gene. As we pointed out...confinement involves building gene drives that can spread through populations of transgenic laboratory organisms...organisms. For example, an sgRNA-only drive will spread exclusively through populations that already express...

  2. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ... the ZFNs.   Genetic Engineering Era: Post CRISPR  CRISPR The strategy of using a protein to recognize... Find more blog posts about CRISPR Learn more about the history of CRISPR Read about other genome editing...methods Resources on Addgene.org Visit our CRISPR guide Find CRISPR plasmids for your research Browse plasmids...during this period.  Genetic Engineering Era: Pre-CRISPR  Having visualized and understood the DNA, the ...breakthrough in the world of genetic engineering as CRISPR-Cas editing soon replaced the protein-based DNA-binding... gene editors (meganucleases, ZFNs, TALENs, and CRISPR-Cas) is that they induce double-strand breaks (...variations in the genome.    Due to the rise of CRISPR in genetic engineering and open advertisement of...

  3. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...       The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids. To...To find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources...delivering CRISPRi components by a single AAV vector are now available from Tonia Rex's lab.  vfCRISPR, or very... very fast CRISPR, is a light-inducible CRISPR system developed by Taekjip Ha’s lab where Cas9-gRNA complex...auxin inducible degron system for C. elegans New CRISPR plasmids New items from the viral service Epigenetic...Saptrap cloning systems to generate AID* tagged CRISPR knock-in repair constructs. These repair constructs...tagged AID* into genomic sites of interests via CRISPR techniques. This tool-kit includes an array of ...

  4. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...off our new CRISPR page (which describes how to plan your CRISPR experiment for our new CRISPR/Cas users... blog. Find more CRISPR and Genome Engineering Resources at our website: CRISPR Resources & Plasmids...genome engineering from ZFNs through TALENs and CRISPR-Cas nucleases, provided important context for the...was followed by three of the biggest names in the CRISPR-Cas9 field – Jennifer Doudna, Feng Zhang, and Keith...on this field. Advances in genome engineering & CRISPR technology That afternoon's workshop, entitled ... way of examining, modifying, and improving the CRISPR-Cas9 system. Mehmet Fatih Bolukbasi from Scot Wolfe's...off-target effects. Other young scientists discussed new CRISPR delivery systems, modifications to the RNA components...

  5. 22 Hot Plasmid Technologies from 2014

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    Blog Post
    ...For more information on these new CRISPR screening tools, see our CRISPR/Cas Plasmids: Pooled Libraries ...Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening. Also, check out our updated CRISPR-Cas ...CRISPR-Cas resources at www.addgene.org/CRISPR/ to browse CRISPR plasmids, watch informational videos, download...for background information on CRISPR technology? See our improved CRISPR-Cas Guide. Wang et al., Science...Tet-inducible CRISPR activators and repressor plasmids. (Kearns et al., Development. 2014..) CRISPRs for Xenopus.... 2014..) Our CRISPR-Cas collection of plasmids updates frequently, so visit our CRISPR pages often to...Microbiology 2011 Feb 22;11:38. Lentiviral CRISPR libraries for knockout screening New systems have...

  6. RUBY-Red Siliques

    Type
    Blog Post
    ...was integrated into a CRISPR system, then tracking the segregation of the CRISPR construct could be achieved...First, red CRISPR-positive transformants can be produced. In the following generation when CRISPR needs to...resources on the Addgene blog  CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis...Arabidopsis Transformation CRISPR 101: CRISPR-Mediated Plant Base Editors...the transgene free individual. For example, with CRISPR-Cas9 gene editing, transformation may be identified...Arabidopsis floral dip). In the next generation the CRISPR construct needs to be segregated away to prevent...currently used in these situations to select against CRISPR positive plants, but screening these can be challenging...

  7. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...days.” Working with CRISPR Recently Doench has been working on developing CRISPR pooled libraries for...technology at the start of the CRISPR craze, but he says, “In the case of CRISPR, the hype was completely justified...biological process X, you can do with CRISPR technology...” and “with CRISPR, just the results are so much cleaner...Podcast.       Resources at Addgene Find CRISPR resources Find CRISPR pooled libraries Learn about other members...understanding, curation, and explanation of our CRISPR resources. We recently sat down with Dr. Doench...and has contributed much of his knowledge to the CRISPR resources at Addgene. His background in history... technology.” Doench thinks that the future of CRISPR will be in high throughput functional characterization...

  8. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...on Addgene.org CRISPR software and resources Addgene CRISPR Guide Addgene’s CRISPR 101 eBook More ...the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: Cas9 The Other...with any other required non-CRISPR-associated host factors required for CRISPR processing. For each enzyme...Expanding CRISPR Beyond SpCas9 More resources from the Innovative Genomics Institute      CRISPRpedia IGI... gene targeting. Fast forward a decade, and the CRISPR field has exploded with dozens of Cas enzymes and... A Functional Classification System for Class 2 CRISPR-Cas Enzymes [Preprint]. Biochemistry, Biophysics..., et al. (2019). Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants...

  9. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ... of Cpf1 Type II CRISPR systems based on Cas9 were thought to be the simplest CRISPR systems and the easiest...is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system." Cell (2015). PubMed PMID: 26422227...Cpf1-driven systems added another option to the CRISPR toolbox. When Cpf1 was first discovered, researchers...nt vs 100 nt.) However, the newly described non-CRISPR protein NgAgo is smaller than any of these options..., so it has the potential to eclipse any of the CRISPR nucleases in AAV-based applications. Get More Cpf1...HDR over NHEJ, so comparing HDR efficiency across CRISPR nucleases and NgAgo is an important next step for...Benjamin P., et al. "Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells." Nature Biotechnology...

  10. SciComm: Taking Science to Elementary and Middle Schools

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    Blog Post
    ...on the Addgene blog Browse our CRISPR blog posts Download our CRISPR cheat sheet Read our science communication...Addgene.org Check out our comprehensive CRISPR guide Find CRISPR plasmids and resources ... we’d already discussed the idea of my giving a CRISPR presentation to their students. I didn’t think ...(which could be adapted for any topic--not just CRISPR): Who am I? Where do I work? The teachers emphasized...this area. What is genome engineering? How does CRISPR work? This section was essentially background slides...protein, plasmid, gene, etc. Though I talked about CRISPR’s molecular mechanism, I was careful to keep it ...teachers and other science communicators.  What can CRISPR do? I spent more time on this section of the presentation...

  11. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ... Addgene Blog CRISPR libraries Genome-wide screens with CRISPR Lentiviral CRISPR libraries ...with small molecules/drugs. With the advent of CRISPR, genome-wide knockout screens are possible with...While the entire genome can be knocked out with CRISPR, the entirety of the small molecule realm is typically...downstream manipulations as a forward or reverse screen (CRISPR, drug, etc.), just with an added baseline component...component. Alternative screening techniques While CRISPR and drugs dominate the screening scene, there are... worth considering. RNAi screens are similar to CRISPR, but instead of knocking out the genes of interest... be lethal to cells and thus not assayable with CRISPR. Another method, popularized in yeast, are temperature...

  12. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    Blog Post
    ... the intended target site. With the widespread adoption of CRISPR/Cas9 technology and rapid improvements...Resources on Addgene.org Browse CRISPR plasmids Check out our CRISPR guide pages Find ready-to-use Cas9...delivery Epitope tagging of mammalian genes using CRISPR depends on successful delivery of three reagents...using custom synthetic two-part cr/tracrRNAs for CRISPR experiments (in collaboration with Integrated DNA...tagging is scalable to 96-well format. Off-the-shelf CRISPR reagents for the knock-in experiment were pre-assembled...csRNP knock-in experiments If your lab is new to CRISPR/Cas9 experiments, there is no need to worry at ...found that 75% of our highest knock-in efficiency CRISPR reagents had crRNAs that bind to the template strand...

  13. An Integrin Antibody Toolkit from IPI

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    Blog Post
    ... IPI and the Springer lab (Hao et al., 2024). In this study, we, along with the Springer lab, reported...produced from hybridoma antibodies developed in the Springer lab. Some are rabbit immunoglobulin G1 (IgG1) ... to be developed and defined. According to the Springer lab, a highly specific function-blocking antibody...   Targeting integrins in the lab The Ha and Springer labs recently showed RGD-binding integrins demanded...higher tensions before they would enable cells to spread, resulting in cells that elongated and morphed ...required and conferred less force, leading cells to spread into symmetrical circles. While elucidating the...integrins. The Ha lab — in collaboration with the Springer lab, the Jaumouillé lab, the Waterman lab, and...

  14. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...for 1-3 days. There’s also a CRISPR for that! CRISPR interference (CRISPRi) targets a catalytically dead...little while Yes, there’s a CRISPR for that, too! CRISPR activators (CRISPRa) are transcriptional activators...approach - read more about them on our CRISPRa blog! Fig. 1 CRISPR activator and repressor mechanisms...knock out the gene for your protein of interest. CRISPR-Cas9 and a custom gRNA is all you need to generate...double strand break. Transient introduction of CRISPRi or inducible expression (more on this below) will... Promoters Plasmid 101: Repressible Promoters CRISPR 101: Which Cas9 Do I Choose for my Experiment ...resources on the Addgene website Promoter Guide CRISPR Guide Lentiviral Guide  ...

  15. Hot Plasmids - October 2020

    Type
    Blog Post
    ....107804 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids. To...To find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources... Addgene The Human GlycoGene CRISPR Library is a new lentiviral CRISPR library that targets human glycosyltransferase...Fluorescent timers for studying cell cycle length The CRISPR corner New from the viral service Labeling excitatory...arrays can lead to artifacts due to overexpression. CRISPR can insert the FP gene into the target gene’s native...introducing large genetic changes in C. elegans with CRISPR can be difficult since it requires the much less...oligodeoxynucleotide donors (ssODN). So how can you use CRISPR to introduce large FP genes into C. elegans? By...

  16. Pooled Library Amplifications

    Type
    Blog Post
    ...Amplification Type Link to Protocol CRISPR Knockout Human CRISPR Knockout Pooled Library (Brunello) ...Amplification Protocol (PDF, 88.2 KB) CRISPR Knockout Human CRISPR Knockout Pooled Library (GeCKO v2) ... Protocol (DOCX, 15 KB) CRISPR Activation Human Genome-wide CRISPRa-v2 Libraries 104,540–209,080...Re-Amplification Protocol (PDF, 71 KB) CRISPR Activation Human Subpooled CRISPRa-v2 Libraries 12,285–20,145 ...Protocol (DOCX, 17 KB)   For CRISPR libraries, Genome-scale CRISPR-Cas9 knockout and transcriptional...ng Solid Joung et al. Nat Protoc. 2017 CRISPR Knockout Human Epigenetic Knockout Library ...on the Addgene blog Genome-Wide Screening Using CRISPR Preparing Pooled Libraries NGS Quality Control ...

  17. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ... other CRISPR/Cas systems used for endogenous protein tagging, please visit our website CRISPR/Cas Plasmids... of interest with a fluorescent protein fusion. CRISPR genome editing can help accomplish this goal. The...sequence (like GFP) can be inserted at the DSB. CRISPR has allowed scientists to tag and light up endogenous...better understand normal protein function. However, CRISPR protein tagging does have its limitations, especially...plasmid assembly toolkit called SapTrap to improve CRISPR genomic tagging in C. elegans. SapTrap offers scientists...systems, including mammalian cells. CRISPaint (CRISPR-assisted insertion tagging), developed by the Hornung...interest to promote homology directed repair after a CRISPR/Cas9 induced break. Learn more about these constructs...

  18. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...The natural CRISPR locus of a bacteria host encodes multiple guide RNAs (gRNAs) on a single array to ... invading phage pathogen. Over the past decade, CRISPR tools have leveraged such host-defense mechanisms...with only minimal payloads. DAP array  Although CRISPR-Cas12a, with abilities to process and release its...leading the edge of the precision gene editing. CRISPR-Cas9, the key component to enable efficient base...multiple gRNAs for nCas9-BE, or any other kinds of CRISPR tools, without compromising their strategic advantages...for potential in vivo applications or multiplex CRISPR screening.   Using tRNAs Luckily, a fun feature... state-of-art base editor, prime editor, or any CRISPR-based gene editors, for correcting mutations or...

  19. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...submitting plasmid(s) using a spreadsheet (right), click the “Download Deposit Spreadsheet” button to download ...(for online submission) or "Download Deposit Spreadsheet" (for emailed submission) in the Deposit menu..."Submit Online Deposit" or "Download Deposit Spreadsheet". Start your plasmid deposit There are two options...Choose to submit plasmid(s) online or by using a spreadsheet. The plasmid information can be entered using...using our online form or by using a downloadable spreadsheet. Either choice can be used for deposits of less... than 10 plasmids but we recommend using the spreadsheet option for deposits of 10 or more plasmids.   ...process and the MTA has been approved. The spreadsheet option is recommended for depositing 10 or more...

  20. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ... Browse CRISPR Tagging Plasmids Browse the Fluorescent Protein Collection Check out the CRISPR Guide Pages...regulatory control. But even with the advent of CRISPR/Cas9 technology, inserting large tags into a precise...labs. We’ve created and tested plasmids that use CRISPR/Cas9 to endogenously tag a wide variety of genes...(HDR) process following the double strand break CRISPR/Cas9 makes at the target site. The whole segment—a...prevent the plasmid from being cut and destroyed by CRISPR/Cas9 during transfection. Making the double strand...introduce the fluorescent tag to the cells, we used CRISPR/Cas9 to make a precise cut in the genome near either...of interest. To get the molecular components of CRISPR/Cas9 (Cas9 protein and the crRNA-tracrRNA complex...
Showing: 201 - 220 of 556 results