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We narrowed to 447 results for: SUP

Showing: 201 - 220 of 447 results

  2. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...gRNA binding a non-mammalian sequence (GAL4) was supplied. In addition to labeling repetitive telomeric...single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector. Figure 2: CRISPRainbow...

  3. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...also improve localization accuracy when used in super-resolution microscopy by reducing the distance between...fast-dissociating antibody fragments for multiplexed super-resolution microscopy. Cell Rep Methods 2(10):100301...

  5. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...know about the vendor and the vendor’s technical support? An antibody from a vendor that you use frequently... with a responsive and knowledgeable technical support team, is a safer bet than one from a large distributor...

  6. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...lab. Granted, I currently work on the Scientific Support Team here at Addgene, so troubleshooting molecular...problem solving skills’? As a part of the Scientific Support team, much of my day is spent helping to resolve...

  8. RNA Extraction Without A Kit

    Type
    Blog Post
    ...plasticware is readily available from scientific suppliers and glassware should be treated with a DEPC solution...material is removed via centrifugation and the supernatant is extracted with chloroform as in the above ...

  9. Summer SciComm: Preprints

    Type
    Blog Post
    ...Some organizations, including some journals, also support open peer review or curation models where editors...Along with other open-science organizations, we support scientists in sharing their results and materials...

  11. 6 Tips for Grant Writing

    Type
    Blog Post
    ...that you ask a PI or mentor (your supervisor/ a previous supervisor / someone else in the department) ...

  12. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...of CpG islands in the promoter region of tumor suppressor genes can repress their transcription and inactivate...al. "Change in paternal grandmothers early food supply influenced cardiovascular mortality of the female...

  14. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...antibodies for IHC. Working with collaborators and support from the Brain Research Through Advancing Innovative...polyclonal antibodies, creating a potentially unlimited supply of identical antibodies (See Fig 1.). rAbs are ...

  16. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...CRISPR screens rely on lentiviral systems, a fact supported by the wide array of lentiviral-based CRISPR libraries...AAV CRISPR screens have been used to map tumor suppressors in mouse liver, interrogate immune cells to improve...

  17. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...plasmids/year from 2017 to 2023. This growth is supported by new labs joining the Addgene CRISPR community...Barrangou for his authorial contributions to, and support for, this and previous Addgene CRISPR publications...

  18. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...AstraZeneca, scientists who get postdocs and do not support or manage them well are simply no longer allowed...the opportunity to do some great science in a supportive environment. Both paths will give you myriad ...
Showing: 201 - 220 of 447 results