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We narrowed to 460 results for: SUP

Showing: 201 - 220 of 460 results
  1. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...biliverdin as a cofactor and so you may need to supplement your cells with biliverdin for maximal brightness...an even newer green fluorescent protein that is supposed to be extremely bright. In S. cerevisiae, we've...
  2. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...go about starting a lab? Obviously, funding and support are crucial parameters, and familiarity with equipment...seek out advice from seasoned PIs who can offer support. “It is an exciting and emotional time!” George...
  3. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...genetic electron microscopy (EM) tag providing superior subcellular localization of tagged proteins compared...yeast display. APEX2 has been shown to exhibit superior performance over APEX for both EM imaging and ...protein expression Under the direction of Sabine Suppmann, the Recombinant Protein Production group at Max-Planck...
  4. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...education and policy and to draw attention and support to work in their field. It can be hugely beneficial...ComSciCon, our primary mission is to strengthen and support the network of graduate students who are passionate...
  5. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...with a kickoff meeting in October, BioBuilderClub supports teams through monthly online group meetings and...the BioBuilder textbook, a laboratory kit, and support during the academic year from remote scientist ...
  6. How Addgene Creates Content

    Type
    Blog Post
    ...conversations, conferences, and questions our scientific support staff receives – in fact, the blog originally started... post (email [email protected].) We are here to support and advance the scientific community, and we can...
  7. Clear and Accessible Writing

    Type
    Blog Post
    ...between layers are muddled due to the passage of supernatant through each layer. The right panel illustrates...before and after ultracentrifugation. The viral supernatant migrates through increasingly dense layers until...
  8. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...also improve localization accuracy when used in super-resolution microscopy by reducing the distance between...fast-dissociating antibody fragments for multiplexed super-resolution microscopy. Cell Rep Methods 2(10):100301...
  9. RNA Extraction Without A Kit

    Type
    Blog Post
    ...plasticware is readily available from scientific suppliers and glassware should be treated with a DEPC solution...material is removed via centrifugation and the supernatant is extracted with chloroform as in the above ...
  10. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...know about the vendor and the vendor’s technical support? An antibody from a vendor that you use frequently... with a responsive and knowledgeable technical support team, is a safer bet than one from a large distributor...
  11. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...lab. Granted, I currently work on the Scientific Support Team here at Addgene, so troubleshooting molecular...problem solving skills’? As a part of the Scientific Support team, much of my day is spent helping to resolve...
  12. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...single-stranded oligonucleotide (ssODN) template supplied by Ma et al., but instead from the mother’s wild...proceeds from the maternal allele rather than the supplied single-stranded oligonucleotide (ssODN) template...
  13. Summer SciComm: Preprints

    Type
    Blog Post
    ...Some organizations, including some journals, also support open peer review or curation models where editors...Along with other open-science organizations, we support scientists in sharing their results and materials...
  14. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...measuring the amount of radioactivity in the culture supernatant (this is indicative of protein degradation). ...variant, DsRed.T3 and a pH-sensitive GFP variant, super ecliptic pHluorin (SEP). They are connected by a...
  15. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...gRNA binding a non-mammalian sequence (GAL4) was supplied. In addition to labeling repetitive telomeric...single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector. Figure 2: CRISPRainbow...
Showing: 201 - 220 of 460 results