We narrowed to 766 results for: VARS

...blogger Jon Chow, an immunology PhD student at Harvard University. In this second post in our quick guide...background. In other cases, YFG or one of its mutant variants will need to be overexpressed or knocked down....Stock Center has fly lines expressing Gal4 under a variety of promoters resulting in tissue specific expression... You can quickly mate your UAS-YFG line with a variety of Gal4 lines and study gene expression in many...Chow. Jon Chow is finishing his immunology PhD at Harvard University. He has had nearly a decade of experience...

...sequences (colored rectangles) are cloned into various plasmids using oligonucleotides. These plasmids...depending on which method you use, the procedure will vary slightly.   Two Golden Gate options that are... with the Gersbach lab plasmids, multiple Cas9 variants are available: wt humanized Cas9, D10A nickase...clone your own entry vectors containing other Cas9 variants to use with the MuLE system. Multiplexing from...into one of 12 pYLsGRNA plasmids, which contain various promoters and reporters, and subsequently inserted...Topic Page Get Advice on Choosing The Proper Cas9 Variant for Your Experiment Learn About Multiplexing with...

...right for me? Here’s a list of some important variables to consider when choosing an inducible promoter...cell types or organisms, promoters are similarly variable. Bacterial promoters only work in prokaryotic ...species from which they were derived. Similarly, the various eukaryotic cell types (mammalian, yeast, plants...target gene? The strength of inducible promoters can vary a lot. If you’re looking for high inducibility, ...time The time needed to induce a given promoter varies. Promoters that require only the addition of an...

...expressing various gRNAs. It’s better to compare the phenotypic effects of expressing various gRNAs in ...choice after transduction with this virus. While various Cas9 mutants have been engineered to perform unique...challenging. This cytotoxicity also causes growth rate variability when using lentiCas9-blast to generate Cas9-expressing... vitro and in vivo (Krashes et al., 2011). The various serotypes available (AAV2, AAV5, AAV8) enable different...

...specific destinations, haul a variety of cargo, and even handle a variety of terrains. They are, in fact..., large collections of biological independent variables, are becoming increasingly popular tools with ...your library cargo to isolate the effects of each variable being manipulated. Thus, the adjustable nature...efforts. And Addgene is here to help! We offer a variety of viral vectors, viral services and even a Viral...

... active variant, the phage is not infectious. The editing speed dictates which phage variants can propagate...complete ABE protein. If the phage delivers an active variant of the base editor, a functional gene III product...stringency PACE, resulted in the evolution of TadA variants with much higher base editing activity compared... TadA-7.10 enzyme. After testing numerous TadA variants, they identified TadA-8e, which edits 590-fold...

...Nabet (behnam_nabet@dfci.harvard.edu), Nathanael Gray (nathanael_gray@dfci.harvard.edu), or Jay Bradner ...an FKBP12F36V binding ligand (ortho-AP1867) via various chemical linkers. Our current lead dTAG molecule...Bradner (james.bradner@novartis.com). Considerations when getting the dTAG system up and running in your lab...While expression of these cereblon neo-substrates varies in different contexts (for example, IZKF1 and IZKF3...

...mosaicism would occur. Although Ma et al. looked at various predicted off-target effects, they limited their... target. In addition, as on-target editing rates vary widely from gRNA to gRNA, it’s unclear where this...ethics committees comprised of individuals with varied backgrounds. Talking about this study with non-... Like me, you’ve probably seen this study (and various articles and commentaries) pop up on Twitter and...CRISPR Topic Page Read about High Fidelity CRISPR Variants Target Multiple Genes at Once with CRISPR Resources...

...relatively pH-insensitive RFP variant, DsRed.T3 and a pH-sensitive GFP variant, super ecliptic pHluorin (...trickier to identify because their contents can be at various stages of degradation. Additionally, electron microscopy...sequence. Under normal growth conditions, both variants are excluded from the vacuole and emit red and...the cytosol-targeted and mitochondria-targeted variants are available from Addgene. References 1. Rosado...

... are created through enzymatic cleavage of the variable region (the antibody region that binds to the ...immunogenic version is needed.  Single-chain fragment variables (scFvs) are ~27kD engineered fusion proteins similar...instead of cleavage. They are comprised of the variable heavy and light chain regions, connected by a ...high-affinity RNA molecules can bind to a wide variety of targets, including proteins, peptides, amino... used either with or as fluorophores in a wide variety of colors.  To antibody or not to antibody?  That...

...co-factors, thus allowing for broad applications in a variety of experiments. LoxP (locus of X(cross)-over in...have been constructed that express Cre under a variety of ubiquitous and regulated promoters, and many... "NNNTANNN", where "N" indicates which bases may vary from the canonical sequence. Among these are loxN...lox2272 (GgATACtT), and lox511 (GtATACAT). These variant lox sites undergo recombination with other sites...not cross-compatible. Using different lox site variants allows Cre to catalyze more than one specific ...

...director of new initiatives at pgEd, I take on a variety of projects in the space of science education, ...genetics pgEd, based at the Department of Genetics of Harvard Medical School, has a mission of raising awareness...ethnic or personal identity. We do this through a variety of activities, including creating educational materials... disciplines, and everyone on staff takes on a variety of projects. With our focus on engaging the community... materials or speak about our experiences in a variety of other settings, including “conventional” academic...

...modified ABE7.10 accordingly which resulted in variants named “ABE8.x”: “8” stands for the eighth round...x-d.  This resulted altogether in 40 new ABE8 variants. When comparing on-target DNA editing efficiency...replaced S. pyogenes Cas9 in the ABE complex with PAM-variant Cas9 proteins: the S. pyogenes NG-Cas9 (PAM: NG...PAM: NNGRRT) to create Sa-ABE8.x-m/d. With these variants, ABE8 could target many additional locations in... base editor: ABE8e Read about xCas9, a CRISPR variant with PAM flexibility Resources on Addgene.org ...

...COVID-19 research in other labs. Spike protein, variants, and pseudotyping The SARS-CoV-2 virus depends...cellular receptor. To more easily study the spike variants, scientists have developed ways to pseudotype ... has had many opportunities to evolve. Several variants are now circulating and plasmids containing these...pseudovirus and those encoding different spike protein variants that can be incorporated into pseudoviruses. The...at neutralizing the different SARS-CoV-2 spike variants. You can learn more about their research in this...

...discussion on effective advocacy strategies on various levels, with panelists including: Kate Stoll, ...Project Lisa Coleman, PhD - Chief Diversity Officer Harvard University  The symposium will culminate with ...appropriate audience and communicating that message in various ways. Workshops will be run by experts in their...Cameron, PhD - Director of Media Communications, Harvard University Ray Howell -Founder of Howell Communications...David T. Riglar. David T Riglar is a postdoc at Harvard Medical School. In the lab, he uses synthetic biology...

...downstream applications. As individual libraries can vary significantly, it’s important that we have an amplification...unique plasmids does your library contain?), the variance of size of plasmids in your library (Are the inserts... cells you need total and per transformation may vary depending on the particular type of cells you are...plasmids to overtake larger plasmids, if the library varies significantly in plasmid size). On plates, each... Library Type Name Number of Plasmids Variable Region Size DNA per Amplification Amplification...

...in a variety of applications including gene expression profiling, studying copy number variation, and ...nucleotide concentration, unlike qPCR where results can vary across replicates. ddPCR can be used to quantify...concentration Gene expression profiling Copy number variations Quantify RNA levels Molecular Diagnostics NGS...of starting material AAV Titrations Allelic Variations Quantifying low abundance targets Monitoring ...

... Throughput Low Low Low Efficiency Variable Variable Variable Difficulty Hard Hard Hard These ...system of choice. The table below summarizes the various components included with each expression system...tunable expression of Cas9 and/or gRNA in a wide variety of mammalian cell lines. Useful for difficult to...

...the thousands of other scientists using pLKO.1 variants learn about those updates? This is a problem many...George Church’s lab). Addgene has also cataloged a variety of useful molecular cloning protocols, including...comments. Currently you can find protocols from NEB, various academic labs, and now Addgene. I just began uploading...currently boasts over 2,500 protocols covering a variety of bio and biomedical topics. Protocol Online Website...resources, such as protocols, Plasmids 101 articles on various plasmid features, and reference tables (i.e. antibiotic...

...the genetic elements may differ (e.g., a set of various mammalian promoters), the same cloning procedure..., titrate inducible transgene expression, or a variety of other clever applications. In one example, ...downstream applications. The tradeoffs between various cloning methods are listed in Table 2. For example...representation. The specific restriction enzymes used in various chaining-based cloning methods are listed in Table...MXS-chaining method have used the technique to engineer various constructs with applications in flow cytometry ...