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We narrowed to 581 results for: Vit

Showing: 201 - 220 of 581 results

  1. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ... a colorful makeover Even without its nuclease activity, catalytically dead Cas9 (dCas9) has many applications... Another caveat of the study is the method’s sensitivity. The authors estimate that a minimum of 150-200...necessary for a detectable signal, limiting the sensitivity of the technique. One potential solution is SunTag...

  2. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...can we shape our time in academia to maximize productivity and success? We asked leading professors for...suggest that you make time for extracurricular activities both to develop new skills and to discover what...about the benefits of pursuing extracurricular activities. Success evolves from passion True success evolves...

  3. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...substrate OR production of a product OR enzyme activity. Small molecule biosensors Addgene has many sensors...constructs are often used to monitor enzymatic activity or phosphorylation over time. You can also use...plate well). Use to track a process (e.g. enzyme activity) over time or under various conditions Localization...

  4. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...include fairground rides and other fun activities and even invite members of the public to attend. We thought...aim to support events and scientific community activities throughout Europe and the world. So get in touch...

  5. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...available allowing you to chemically control neuronal activity while studying your phenotype of interest. Optogenetic...Optogenetic tools for similarly controlling neuronal activity with light will be available soon. We’re also ...sharing will only help research advance. Remember: productivity is infectious!  Additional Resources on the...

  8. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...resource list available from this post. Use an activity to start a discussion. The Forced Choice Analysis... can use it to clarify and discuss values. The activity is just the start – it is the discussion after...mentoring blog posts, the "Forced Choice Analysis Activity", the "Peer Mentor Groups Guide", "Tips for Choosing...

  10. Rett Syndrome

    Type
    Collection
    ...suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling,...

  13. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...transduction profiles in different AAV serotypes both in vitro and in vivo when compared to the conventional AAVs...et al., 2017), exo-AAV2 in mouse retina after intravitreal injection to transduce bipolar cells and photoreceptors...robust gene delivery into the murine retina upon intravitreal injection." Scientific reports 7 (2017): 45329...

  14. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ... on bacterial expression systems here) and the activity of the plant compounds on these proteins will ... be tested. Investigating changes in neuronal activity after ischemic brain injury Dr. Gohar Tsakanova...image cortical neurons and study changes in neural activity after ischemic brain injury. Additionally, optogenetic...

  15. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...relationship with authenticity and not discomfort: Invite the new contact to connect on LinkedIn and always...make the relationship stronger.  If appropriate, invite them to speak for your organization, mentoring ... simple question so I accepted the connection invitation. They now message me frequently and ask me how...

  16. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...we are missing is the unmeasurable skills and productivity coming from that casual discussion on the way...this is an underrated ritual that really drives creativity and novelty in scientific research and in any...springboard for future discussion. Collaboration and creativity are pivotal in writing publications/grants too...

  17. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...free to anneal to complementary ssDNA molecules in vitro. Figure 1: Cas9 binding to the cleaved DNA... if we could leverage this model (built from in vitro studies) to boost the efficiency of sequence replacement...enables sequence replacement without cutting Our in vitro results also demonstrated that catalytically inactive...

  18. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...from the 3’ end, while its native endonuclease activity degrades the displaced strand.   Figure... the nick site, which was expected since the processivity window of PolI is 15-20 bp. EvolvR’s on-target...bacteriophage T7 DNA polymerase increased the processivity of PolI3M, and thereby increasing the editing...

  19. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...encoded calcium indicators (GECIs) have become a vital tool in neuroscience research. These fluorescent... calcium levels change rapidly during neuronal activity, scientists can use GECIs to watch neurons fire...fluorescent calcium ion indicator for imaging neural activity. bioRxiv 2025.07.28.667269; doi: https://doi.org...

  20. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...from AAV2 because they demonstrate high promoter activity (Earley et al. 2020), but non-AAV2 ITRs can be...within Inverted Terminal Repeats (ITRs) on the Productivity, Genome Packaging, and Potency of AAV Vectors...regions of inverted terminal repeats reduces rAAV productivity but increases transgene expression. Sci Rep,...
Showing: 201 - 220 of 581 results