We narrowed to 577 results for: Vit
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TypeBlog Post...these. Addgenies have been organizing Halloween festivities for years – searching our photo archives I found...bragging rights for the next 12 months. Your team’s creativity, cleverness, and crafting skills have surpassed...tribute to the changing seasons – and they also invited Father Time to the party. They won over our judges...
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Congratulations, Deck The Lab winners!
TypeBlog Post...annual Deck the Lab competition drew upon the creativity of the community, who delivered impressive science-themed...In third place is Matthijs Dorst’s Deck the Lab Nativity scene below his Christmas tree! It is that time...up at home!This year with a #DeckTheLab themed nativity scene, courtesy of our house pets.. 😁 pic.twitter.com... -
What's New in CRISPR - Winter 2018
TypeBlog Post...developed a platform called SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) to detect single...further to provide multiplexed detection, higher sensitivity, and more. Read the paper in Science Find...dual-nuclease Cas9-Cas9 chimeras with higher target site activity and more predictable and precise deletion products... -
Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton
TypeBlog Post... other projects, using stem cells to develop in vitro models for lung disease. Darrell strives to promote...reliable projects. Chapter 9: Using stem cells as in-vitro models of disease. How Darrell is using stem cells...reagents, cell lines, data, and knowledge without exclusivity. Chapter 11: The Stem Cell Bank (CReM Bank) at... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post... a colorful makeover Even without its nuclease activity, catalytically dead Cas9 (dCas9) has many applications... Another caveat of the study is the method’s sensitivity. The authors estimate that a minimum of 150-200...necessary for a detectable signal, limiting the sensitivity of the technique. One potential solution is SunTag... -
9 Tips to Achieve Success in Academia
TypeBlog Post...can we shape our time in academia to maximize productivity and success? We asked leading professors for...suggest that you make time for extracurricular activities both to develop new skills and to discover what...about the benefits of pursuing extracurricular activities. Success evolves from passion True success evolves... -
Fluorescent Proteins 101: Fluorescent Biosensors
TypeBlog Post...substrate OR production of a product OR enzyme activity. Small molecule biosensors Addgene has many sensors...constructs are often used to monitor enzymatic activity or phosphorylation over time. You can also use...plate well). Use to track a process (e.g. enzyme activity) over time or under various conditions Localization... -
Networking and Inspiring Young Scientists at Freshers Fairs
TypeBlog Post...include fairground rides and other fun activities and even invite members of the public to attend. We thought...aim to support events and scientific community activities throughout Europe and the world. So get in touch... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog Post...several important shortcomings including low processivity (ie. the probability of the polymerase continuing...heterogeneous RNA samples. Furthermore, such high processivity enzymes and the structural understanding thereof...to existing technologies based on polymerase processivity. Please find the MarathonRT plasmid here! Zhao... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post...purity or infectivity of the final viral preparation. Through sugar detection assays, in vitro transduction...show that there is no difference in purity or infectivity between the untreated and sucrose treated viral... -
Using Addgene's New Viral Service for Your Research
TypeBlog Post...available allowing you to chemically control neuronal activity while studying your phenotype of interest. Optogenetic...Optogenetic tools for similarly controlling neuronal activity with light will be available soon. We’re also ...sharing will only help research advance. Remember: productivity is infectious! Additional Resources on the... -
Mentoring for Scientists: I Have a Mentor, What Now?
TypeBlog Post...resource list available from this post. Use an activity to start a discussion. The Forced Choice Analysis... can use it to clarify and discuss values. The activity is just the start – it is the discussion after...mentoring blog posts, the "Forced Choice Analysis Activity", the "Peer Mentor Groups Guide", "Tips for Choosing... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog Post...allow researchers to independently control the activity of two populations of neurons at once. Addgene...is a toolset that lets you turn the electrical activity of neurons on or off using pulses of light. By...both activators as well as silencers of neural activity. Since then, there has been a steady stream of... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...genome editing tool. However, Cas9’s endonuclease activity can be limited by the need for a specific PAM ...are ion channels that can be used to silence the activity of neurons. Earlier this year, the Hegemann lab...valuable optogenetics feature when studying neuronal activity. The researchers identified MerMAIDS from their... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog Post... Chiasson is currently using VAMP-seq to study vitamin K epoxide reductase (VKOR) variants. VKOR is an...assays for measuring human serotonin transporter activity. Unger also plans to use the serotonin receptor...genotype the patient and rapidly test the variants in vitro against different drugs and dosages. Michal Bernach... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...transduction profiles in different AAV serotypes both in vitro and in vivo when compared to the conventional AAVs...et al., 2017), exo-AAV2 in mouse retina after intravitreal injection to transduce bipolar cells and photoreceptors...robust gene delivery into the murine retina upon intravitreal injection." Scientific reports 7 (2017): 45329... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post... on bacterial expression systems here) and the activity of the plant compounds on these proteins will ... be tested. Investigating changes in neuronal activity after ischemic brain injury Dr. Gohar Tsakanova...image cortical neurons and study changes in neural activity after ischemic brain injury. Additionally, optogenetic... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog Post...we are missing is the unmeasurable skills and productivity coming from that casual discussion on the way...this is an underrated ritual that really drives creativity and novelty in scientific research and in any...springboard for future discussion. Collaboration and creativity are pivotal in writing publications/grants too... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog Post...relationship with authenticity and not discomfort: Invite the new contact to connect on LinkedIn and always...make the relationship stronger. If appropriate, invite them to speak for your organization, mentoring ... simple question so I accepted the connection invitation. They now message me frequently and ask me how... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog Post...free to anneal to complementary ssDNA molecules in vitro. Figure 1: Cas9 binding to the cleaved DNA... if we could leverage this model (built from in vitro studies) to boost the efficiency of sequence replacement...enables sequence replacement without cutting Our in vitro results also demonstrated that catalytically inactive...