We narrowed to 268 results for: aav
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TypeGuide...they drive expression. Different AAV serotypes can be used to target AAV vectors to specific cells of interest...considered. For tissue specificity of AAV serotypes, see our AAV guide . FLEx Vectors are also used to...models. For viral injection, Adeno-associated virus (AAV) is a widely-used tool for achieving in vivo expression...of chemogenetic receptors, and a wide variety of AAV-encoding chemogenetics plasmids are available. Additionally...available. Browse our collection of Ready-to-use AAV Preparations of Chemogenetic Plasmids ! Table 4. ...Targeted expression. Depending on the experiment, an AAV-encoded chemogenetic plasmid may need to be targeted...regions of a neuron. Cell-specific expression of AAV-delivered constructs can be controlled with cell-...
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22 Hot Plasmid Technologies from 2014
TypeBlog Post...been deposited to Addgene, including lentiviral and AAV expression vectors for certain variants. Klapoetke... infeasible. Finally, an adeno-associated viral (AAV) system enables greater spatio-temporal control over... either the Thy1 or CAG promoter, while Brainbow AAV is under control of the EF1a promoter. Livet et... -
Plan Your Experiment
TypeGuide... collection of in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector...delivery. Both lentiviral and AAV vectors are safe to use in the lab, but AAVs exhibits the lowest immunogenicity... transgene expression with AAVs can be long-lived, despite recombinant AAVs not integrating into the host...also frequently use AAVs to develop CRISPR-based therapeutics. One limitation of AAV vectors is that the...blog post on overcoming the AAV size limitation . Browse Addgene's in-stock AAV preps or try Addgene's Packaged...delivery, lentiviral and adeno-associated virus (AAV) vectors are the most common. Using viral vectors...vector) is smaller than that of lentivirus. Thus, AAVs are generally only compatible with smaller Cas enzymes... -
27 Hot Plasmids from 2016
TypeBlog Post... 74307 pAAV-CMV-DIO-Magneto2.0-sNRPpA AAV 74306 pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA AAV 74302 ...plasmids, which already includes dSaCas9-VPR in an AAV transfer vector for in-vivo expression and plasmids...Magneto2.0 in the mammalian brain after delivering it via AAV injection, finding that it could increase the activity...lines by inserting OsTIR1 into the "safe harbor" AAVS1 locus (a tet-inducible OsTIR1 plasmid is also available... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...lentivirus, Adenovirus, and Adeno-associated virus (AAV). If you are wondering which virus fits you experiments... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post...it easier to package for therapeutic delivery by AAV, which has strict capacity limitations. New efforts...Science, 362(6416): 839-842 (2018). 10.1126/science.aav4294 Ozcan, Ahsen, Krajeski, Rohan, Ioannidi, Eleonara... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...effects on neuron activity (firing or inhibition). AAV preps from DREADD plasmids allow you to easily express... -
Depositor Collections
TypeCollection...Collection - Davidson Allen Institute for Brain Science AAV Enhancer Collection Allen Institute for Cell Science... -
CRISPR Plasmids - RNA Editing
TypeCollection...capabilities but are small enough to be packaged in AAV particles. Want more information on the wide variety... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...plasmids were developed to replace helper viruses in AAV production systems. We’re just skimming the surface... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post...effects of various terminators in the context of AAV transgene expression levels. Transcription Termination... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...AdVs have already a packaging capacity larger than AAV vectors. This capacity is large enough to carry the... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...acids) means they can be delivered in vivo using AAV vectors. Having a way to shut off CRISPR-Cas systems... -
PCR Applications for Copy Number Variation Assays
TypeBlog Post...Resources on the Addgene blog Droplet Digital PCR for AAV QuantificationPolymerase Chain Reaction Overview ... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...targeting expression involve tissue specific promoters, AAV serotypes, and the ever-growing variety of recombinase...applications. Science 6436: eaav5282. https://science.sciencemag.org/content/364/6436/eaav5282.long Redfern CH, ... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post...to their transduction has been shown to increase AAV transduction efficiency by approximately 15-fold ... -
Plasmid Collections
TypeCollection...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated... -
Hot Plasmids: Winter 2025
TypeBlog Post...Lee et al., 2024. The authors have deposited AAV plasmids for Cre-dependent and -independent versions...for the first time (Figure 3). The team also used AAV9 to deliver ASAP5 to the cortex and imaged voltage... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...amplify! Examples of this are the GC-rich ITRs of AAV genomic DNA encoding plasmids, or promoters such ... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...Plasmids Read Our Lentiviral Vector Guide Read Our AAV Guide ...