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...The Addgene-Seeding Labs plasmid grant is a collaborative endeavor providing plasmids to researchers in... of Zimbabwe, Zimbabwe Dr. Idah Sithole-Niang (above, left) is an Full Professor in Biochemistry with... CRISPR effect observed in bacteria. Learn more about the different CRISPR applications in plants.     ...Rodrigo Arturo Rivera Solís, Associate Professor (above, far right), thrives to make affordable biosensors...detect glyphosate via H2O2, one of the reaction’s metabolites. One useful genetically encoded H2O2 probe is... to the Addgene blog for updates and future collaborations. Many thanks to all of the awardees, all who...Additional Resources on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out...

... will also serve as a good mentor. To read more about what makes a good mentor, see the previous post ... and personnel management?  If you are thinking about a non-academic path, will the PI support this path...Medical School researcher Connie Cepko to learn about her mentoring style. How can I find out if an advisor...time with the potential advisor and don’t be shy about asking hard questions. Talk to as many lab members... past lab members. Most scientists love to talk about their past lab experiences and will be willing to...members, neighbors or alumni give you information about the PI that concerns you, don’t just brush this ...Mentors by @drmellivora, an excellent blog post about workplace bullying featured on the always interesting...

...Cell tracing in zebrafish with Zebrabow Before the generation of Zebrabow (Pan et al., 2011), Brainbow ...crossing of Zebrabow animals to an inducible Cre line. The Schier group developed Zebrabow as a system...by injecting Brainbow at the single-cell stage. Zebrabow, or “zebrafish brainbow,” is a collection of transgenic...are introduced using a plasmid construct called Zebrabow-GateDest containing an RFP, CFP, and YFP downstream...downstream of a ubiquitous promoter. Zebrabow is an extremely versatile technology as recombination can be...an introduction to live cell imaging Learn more about the origin of the Brainbow system Visit our fluorescent... Addgene’s fluorescent protein collection Learn about fluorescent tools for in vivo imaging ...

...a successful negotiation and collaboration. We’d love to hear more about your experiences and opinions...can get away with using computer modeling and collaborations rather than using animals, then that’s a cost...associated with old equipment, like service repairs, labour costs, and loss of time, can exceed the cost of...Everything is negotiable. Try asking for discounts, collaborations, access to internal award applications, etc...This is a non-profit organization. Therefore, collaboration and community is the ultimate value proposition...Happy Sciencing, Damien   Damien Wilpitz is a laboratory research management consultant and coach in Boston...research scientists create productive and manageable laboratory environments. His business skills enhance his...

...communication in remote collaborations Transferable Skills Guide: External collaborations Transferable Skills... stint may be ideal for you. Here, we will chat about who might want to explore this role, how to seek...scientist is distinct from equipment sharing or a collaboration – it requires re-location of the scientist to...reasons Visiting gives you access to possible new collaborations and fresh perspectives. For trainee level researchers...future work placements. The chance to learn and collaborate all while experiencing a new city or maybe even...there is someone you personally know or your lab collaborates with that can fulfill your research needs, that... open to sharing knowledge and establishing collaborations, so it doesn’t hurt to reach out and ask if...

...the Addgene Blog Learn about Efforts to Fight Rare disease with iPSCs Read About Addgene's Outreach Efforts...the first clinical trial with iPSCs with his collaborator Takahashi in 2014 – just 8 years after the OSKM...researchers special: 1. Stem cell scientists care deeply about ethics Ethics must play a prominent role in the ...Researcher Darrell Kotton 2. Stem cell scientists care about patients As any grad student who has spent years... a big picture perspective: Dr. Yamanaka talked about his clinical trial patients with advanced macular...proof that these researchers are thriving in a collaborative, rather than a combative, field. Of course they... in the Microbiology Community Sign Up to Write About Stem Cells Resources on the Addgene Wesbite Find...

...fully-equipped laboratory for citizen scientists. In addition to maintaining a laboratory to support both... of independent Do-it-Yourself (DIY) biology laboratories working towards enabling everyone to participate...Anyone can become a member of BosLab and previous laboratory experience is not required. Our members are skilled...have non-traditional scientists working in our laboratory! Don’t worry though, we make sure that everyone...samples. The goal is to not only learn something about the microbial diversity in different soil conditions...community lab in a city near you. To learn more about what we’re up to or how you can help BosLab, visit...a co-organizer at BosLab, a community biotech laboratory in Somerville MA. Follow her on twitter at @ZebraElement...

... communication, and in particular communication about experimental methods and approaches. It’s very easy...everything will be fine. It won’t. When we hear about issues with reproducibility, time and time again...the problem comes down to inadequate information about what the original researchers actually did. Admittedly...Communication comes in many forms—from transparency about data to allow others to see what you actually found...fits together to make for a transparent and collaborative research environment. Journals as enforcers ...of methods sections, including clear information about reagents, and why other publishers and organizations... in reproducibility jail and more effective collaboration to drive the progress of science. Sounds like...

...see the new map in action). Read on to learn more about the improvements we’ve implemented and how they’...more functional display, you can start thinking about your next cloning experiment early on. Beyond these... to to download the static image file (PNG, see above) for your own reference or to paste directly into...identified a plasmid that you want to learn more about, updates to our sequence analyzer will allow you...the features shown in the static maps discussed above, but also have interactive functionalities. You ...enzyme, primer, or ORF to learn more information about it. For example, if you hover over the ampicillin...guide A quick guide to all of the tabs listed above and their functions. We hope that these updates...

...designed (listed above) worked well and I got clear bands using the conditions above. 2. Design your primers...essential for experiments but that nobody talks about. After months of troubleshooting, those people who...details. The entire process from start to finish took about 1 month. Cas9 delivery Overview: Before any ...is a loading control. Pro-Tips: 1. It’s all about the MOI. In retrospect I used a way too high Cas9...original publication they see GFP expression in about 20% of their Cas9-expressing cells. So, even when... gene has a weird mutation that you didn’t know about (which could affect the ability of the gRNA to bind...engineering has come. For comparison, in 2007 it took about 3 months to knockout a single allele in a human ...

...Addgene Blog Learn about Synthetic Biology with BioBuilder Use Addgene Videos to Learn about Basic Lab Techniques...species, but these studies generally leave questions about gene function wide open. What if two seemingly similar...been using yeast models (3, 4) to answer questions about the evolution of the genes involved in methionine...question, the plasmids are also used to teach students about restriction mapping and western blotting. Students...communicating science to the public. You can find out more about Clare at the Biology Dept. site.  References 1. ...PMC4596657. 5. Thomas, D., & Surdin-Kerjan, Y. Metabolism of sulfur amino acids in Saccharomyces cerevisiae...Functional genomics research in an introductory laboratory course. CBE-Life Sci. Educ. 17, ar1. (2018). ...

...the Addgene Blog Learn More about Homology Directed Repair Learn More about Non-Homologous End Joining .... For a C-terminal tag, as shown in the example above, we inserted a linker and GFP tag at the end of ... and 1kb of homologous DNA flanking both sides (about 2.7kb total)—is inserted into a plasmid backbone... Plasmids, plasmids everywhere To find out more about gene edited hiPS cell lines generated using the ...line. The editing strategy and design described above can be used to create similar donor plasmids for...microhomology-mediated end-joining (MMEJ). To find out more about gene edited hiPS cell lines generated using these...

...introduction to my area, a line about the research I will be doing, and a line about what I hope to discover....themselves many times and will have useful advice about what reviewers in your area are looking for. Remember...One way to add originality to your work is to collaborate with specialists in different fields that can...the field. 3. Highlight your network and your collaborators It’s a good idea to move around to different...outcomes can include poster presentations, talks about your work, or even the successful use of your preliminary...quality application. Even following this advice, only about 50% of my grant applications have been successful...on your grant application to your peers and collaborators can also help you get some critical insight ...

...more about Dr. Kwapata’s current work and thoughts on the need for more scientific collaboration across... connecting us with him. Learn more about our recent collaboration with Seeding Labs here. Special thanks...received an award from nonprofit and Addgene collaborator, Seeding Labs, for lab equipment that will help...Addgene Podcast.     Additional Resources Learn about Seeding Lab's Work with Lilongwe Univeristy of Agriculture...How You Can Contribute to the Addgene Blog Learn about Addgene Plasmid Donations for Rare Disease Research...

...negotiate with friends, partners, and/or children about everything from what you’re going to eat for dinner...Negotiation as a zero sum game Some people think about negotiation as a zero sum game - that is, if I get... three examples depend on many specific details about the individuals, each example provides a scenario... difficult because of the need to be thoughtful about what is most important to each person. It can especially...of us associate with negotiating (check out more about conflict resolution). How to develop negotiation...before, during, or after a negotiation to think about the situation, your reaction, and the outcome. How... able to take my vacation? I want to learn more about a different area of the company. How can I negotiate...

...Blog Learn about Engineered Cas9 PAM Variants Read about CRISPR Base Editing Learn about Homology Directed...editing specificity. We’re excited to learn more about xCas9 - here’s what we know so far! Why do we ...point mutations, also has a strict editing window about 15 nt upstream of the PAM. Even if there’s a PAM...in mammalian cells, finding xCas9 3.7 displayed about the same editing rate as SpCas9 at an NGG PAM. When...are exciting, there’s a lot we still don’t know about xCas9. “I’m not 100% sure xCas9 is going to be flat...Find CRISPR Plasmids for Mammalian Systems Learn about CRISPR History ...

...record readership with over 75,000 views. Changing Labor Laws Bring Increased Postdoc Wages Our most popular... for anyone considering a postdoc. In “Changing Labor Laws Bring Increased Postdoc Wages,” Future of Research...thorough yet concise rundown of how recently enacted labor laws will likely increase postdoc earnings in the...changes quickly and harmlessly. Learn much more about the laws and their possible repercussions in Gary...This post leads readers to our latest podcast all about graduate student life and how establishing a thriving...to Use Reddit for Science Communication Changing Labor Laws Bring Increased Postdoc Wages I hope you all... labor laws...

...think about. Choosing a topic and gauging interest The first thing to do if you’ve thought about starting... discussions. Beyond the excitement of learning about new cutting edge science, researchers of all varieties...help stay current on new and emerging work by collaboratively discussing recent publications in a specific...up. At Addgene, we wanted to think more closely about the wealth of high-throughput sequencing (HTS) data...the paper.  Before the first journal club, think about the meeting itself A journal club should aim to ...published science. They also serve as a way to learn about work outside of one's own field in a structured ...hope that with some of the information described above you feel empowered to start your own journal club...

...wonderful tangents. Some groups asked about vaccines, others were curious about the agricultural repercussions...roles and responsibilities, I have to think a lot about how to balance those roles. I’m a father of three...community. This past year, I spent some time thinking about how I could combine all of these roles by reaching...guest presentations. If you’re a scientist thinking about reaching out to your community’s schools, here are...accountants running our math tables!  Be realistic about what you can teach  5-10 year olds in a single evening...DNA, protein, plasmid, gene, etc. Though I talked about CRISPR’s molecular mechanism, I was careful to keep... I gave my presentation five times to groups of about 50 students each, and each group went on its own...

...for gene manipulation together with information about the genome are powerful resources for investigating...zebrafish genome was published in 2013, revealing that about 70% of human genes have a counterpart in zebrafish...networks that regulate processes from development to metabolism to fertility. Several zebrafish-specific gene...gene expression databases that provide information about when and where genes are active in developing embryos...genome sequences become available, new hypotheses about specific genetic interactions arise and tools to...genome, their long three-month generation time and labor-intensive maintenance compared to invertebrate model...Patrick Müller’s group at the FriedrichMiescher Laboratory of the Max Planck Society in Tübingen, Germany...