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We narrowed to 397 results for: abo.4

Showing: 201 - 220 of 397 results

  1. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ...1335–1347. https://doi.org/10.1038/s41590-019-0480-4 LaFleur, M. W., Nguyen, T. H., Coxe, M. A., Yates,...LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in...K.B., Gillis, J.E., Sen, D.R., Gaudiano, E.F., Al Abosy, R., Freeman, G.J., Haining, W.N., & Sharpe, A.H...

  2. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...641–656. https://doi.org/10.1038/s41579-022-00739-4 More resources on addgene.org  Addgene's CRISPR Guide...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...

  3. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...–8 weeks Average shipping time: 1–2 days (USA); 3–4 days (international; varies widely by country and/...educational resources.  We hope you’re as excited about this expansion as we are! Happy packaging! ...

  5. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...: a quantitative comparison . Molecular Therapy, 4 (4), 383–391. https://doi.org/10.1006/mthe.2001.0456...packaging capacity of rAAV vectors to ~2.4 kb. Figure 4: Examples of AAV serotypes, pseudotypes, hybrid capsids... . Current Opinion in Molecular Therapeutics, 11 (4), 442–447. PMID: 19649989 (Link opens in a new window...for human gene therapy . Current Gene Therapy, 3 (4), 281–304. https://doi.org/10.2174/1566523034578285...AAV) as a vector for gene therapy . BioDrugs, 31 (4), 317–334. https://doi.org/10.1007/s40259-017-0234...our adeno-associated viral vector guide to learn about AAV vector components, production and common uses...E2a and VA for replication. For more information about adenovirus, read our Adenoviral Vector Guide . Recombinant...

  6. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...manuscripts. 4. Balance focus and exploration Researchers face a fundamental conflict. Science is about exploration...have finite time and resources. You typically have 4-5 years to make discoveries and publish papers to ...Nature and Science, for example). This blog post is about how to get the most out of your postdoctoral training...your new model?  PFs need to be regularly thinking about manuscripts. The other items will follow. If you...this is a strong indicator that this person cares about your science and your development. Taken together...many PFs are reluctant to give talks or posters about their research. They dread giving a departmental...manuscript reviews, etc. You'll also meet future collaborators and get access to unpublished information and...

  7. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...settings are often limited to off-target sites of <4 bp. The off-target list is also generally weighted...PMID: 25409780. PubMed Central PMCID: PMC4246457. 4. Davidson CJ., et al. "Improving the limit of detection...et al. 2015). Meanwhile, Sanger sequencing is laborious, time consuming and cannot be applied to heterogenous...viable options for multiplex sequencing in many laboratories. Nevertheless, unbiased detection isn’t as sensitive...unbiased sequencing may not be worth the cost and labor when single clones are selected. Conversely, translational... Hough is a Biochemistry PhD candidate in the laboratory of Prof Steve Jackson at the Gurdon Institute...

  8. CRISPR Guide

    Type
    Guide
    ...double-strand break (DSB) within the target DNA, located ∼3–4 nucleotides upstream of the PAM sequence. The resulting...Modifications Using Homology Directed Repair (HDR) Figure 4: Overview of HDR HDR generates specific genomic edits... for In Vivo Genome Editing. Molecular Cell , 73 (4), 714-726.e4. PMID: 30581144 Fu, Y., Foden, J. A.,...engineered CjCas9 orthologs. Molecular Therapy , 31 (4), 1177–1187. PMID: 36733251 Harrington, L. B., Doxzen...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman,...CRISPR/CAS-Mediated Genome Engineering. Cell , 153 (4), 910–918. PMID: 23643243 Zhang, Y., & Marchisio, ...cytidine deaminase fusions. Nature Biotechnology . 35 (4), 371–376. PMID: 28191901 Komor, A. C., Kim, Y. B....

  9. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ... is needed to read your experimental output; and (4) the consequences to your experiment if there’s off-target...neurons (NeuN). Image from Griffin et al., 2019, Figure 4. Used under Creative Commons Attribution 4.0 International...starting the selection process. Factors to think about are (1) your experimental conditions and measurements...

  10. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...Scientists on Twitter here. 4)   If you enjoy writing and are ready for a larger commitment, start your own...take on collaborative roles in academia, and that means it’s important to know how to talk about science...talking about and sharing some science on the one you already have. Read my earlier post about Scientists...important for scientists to get out there and talk about their work. To tell others why that work is cool... than ever before for scientists to communicate about their work and their interest in science more generally...already make it a habit to talk – really talk – about the research you are doing in the lab with your ... the details of your science but also something about how science really works. 2)   Read science writing...

  11. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    ... getting abstracts and plans together for my next 4 webinars—I have them stacked close together for the... post if you debating about an academic or non-academic career Learn more about alternative science careers... written for selfish reasons: I wanted to learn about careers in science communication. When I started...Communication Internship with Addgene, I didn’t know a lot about scicomm, but had enjoyed writing a few Addgene guest...option. So if you’re interested in learning more about science communication careers, you’re in the right...careers here Resources on Addgene.org Learn more about careers at Addgene ...

  12. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...distributed – have changed so dramatically in the past 4 years. I kept busy for the whole poster session, talking...It's been about 14 years since I last attended a Keystone Meeting – far too long. Holding these meetings...CRISPR technology in just 2 years and excitement about how much more there is left to do with the characterization...downstream output. Tuesday morning's talks were also about circuitry and synthetic biology, and I found myself...plasmid sharing to those who were anxious to hear about what was coming next. I also got to show off our... updated Synbio page to people who were curious about these newest features of our website.  I want to...

  13. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ... and technology for bioprocesses (1989): 272-278. 4. Ozturk, Sadettin S., and Bernhard O. Palsson. "Effect...resources on Addgene.org Learn about Addgene's viral service Learn about viral production Find viral protocols... By now you have probably heard about Addgene’s viral service but did you know that Addgene also performs... approach can be adapted by vector cores and laboratories alike as an easy and inexpensive method to improve...Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production." Biotechnology and ...

  14. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...fields not solely the specific area the writer works.  4. Practice writing everyday Overall, the most important...successful writer. When I originally talked to my PI about becoming a better writer, I was unsure if I should... a manuscript and critiquing it, were not too laborious, but helped build my understanding of how to generate...and the writing workshop. SWPI's writing workshop About a month later I received another email notifying... manuscript, scientists have a tendency to be elaborate and over explain the material. The point of writing...

  15. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ... you can use to express your protein of interest. 4. Plasmids 101: Blue-White Screening Getting a lot ...eyes open for any new technologies that will bring about the next revolution in biological research. We’re...Plasmids 101: Control Plasmids Need help thinking about the types of controls you’ll need for your next ...Addgene Browse Our CRISPR Resources Learn More about Viral Vectors Browse the Repository  ...

  16. Lentiviral Vector Guide

    Type
    Guide
    ...as well. Third-generation plasmids include (Figure 4): Transfer plasmid — contains transgene and LTRs (...third-generation systems, please refer to Table 1. Figure 4: Third-generation lentiviral plasmids. Created with...Vectors through Pseudotyping. Current Gene Therapy , 5 (4), 387–398. https://doi.org/10.2174/1566523054546224...gene silencing by RNAi in primary cells. RNA , 9 (4), 493–501. https://doi.org/10.1261/rna.2192803 PMID..., 21 (1). https://doi.org/10.1186/s12864-020-6647-4 PMID: 32151239 Zufferey, R., Dull, T., Mandel, R. ...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...lentiviral vectors that can be safely used in laboratory settings. These engineered lentiviral vectors...

  17. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...cytotoxicity against tumor targets. Nat Med. 2000 Apr;6(4):443-6. doi: 10.1038/74704. PMID: 10742152. Dunkelberger...phagocytosis.  Other functions We’re still learning about Fc effector functions and all that antibodies can...isotypes and Fc receptors that we don’t know much about yet. Who knows what kinds of functions they may ...

  18. RUBY-Red Siliques

    Type
    Blog Post
    ...be collected from heterozygous individuals. Only 1/4 of the descendants of the heterozygous plants, therefore... is difficult for new members of the lab or collaborators unfamiliar with these mutant plants. Using RUBY...resistant disease. (RUBY-red: You can’t beet it!) About the Author  Andrew Willoughby is a graduate student...

  19. Chemogenetics Guide

    Type
    Guide
    ...FFz, CFz). Learn more about optogenetic systems in our Optogenetics Guide . Figure 4: Luminopsins can be...L131G, Q139L, and Y217F and is termed PSAM4 (or PSAM 4 ). Additional varenicline analogs, termed ultrapotent...AAV Preparations of Chemogenetic Plasmids ! Table 4. Common promoters in chemogenetics plasmids Promoter...controlled with cell-type specific promoters. Table 4 lists some common promoters found in chemogenetic .... However, CNO is back-metabolized to clozapine and other clozapine metabolites and these can have off-target...concentrations of clozapine N ‐oxide (CNO). CNO is a metabolite of the antipsychotic clozapine and seems to be...experiments in this species may be entirely due to back-metabolism of CNO to clozapine, demonstrating the need for...
Showing: 201 - 220 of 397 results