We narrowed to 411 results for: abo.4

...knocked out in the insulin signaling cascades in only 4 months compared to a year or greater with older technologies...be used in people until we learn more about safety and more about the how to use genome edited stem cells...because Jennifer did her graduate work one floor above mine in the lab of Dr. Jack Szostak, who went on...ceremony honored Charpentier and Doudna, who talked about their research leading up to the discovery of the...Charpentier met at a 2011 conference and decided to collaborate on what was a “curiosity” driven project. Both...importance of the strong, cross-disciplinary collaboration they shared to the work they did together. They...

...1335–1347. https://doi.org/10.1038/s41590-019-0480-4 LaFleur, M. W., Nguyen, T. H., Coxe, M. A., Yates,...LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in...K.B., Gillis, J.E., Sen, D.R., Gaudiano, E.F., Al Abosy, R., Freeman, G.J., Haining, W.N., & Sharpe, A.H...

...641–656. https://doi.org/10.1038/s41579-022-00739-4 More resources on addgene.org  Addgene's CRISPR Guide...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...

...–8 weeks Average shipping time: 1–2 days (USA); 3–4 days (international; varies widely by country and/...educational resources.  We hope you’re as excited about this expansion as we are! Happy packaging! ...

...involved in bacterial toxin resistance, identifying 4 previously unknown genes.  “With this work, it is ...Biotechnology. 2013 Dec 23. (Article). Read more about CRISPRs: Using CRISPR/Cas to Edit Disease Out of...

...manuscripts. 4. Balance focus and exploration Researchers face a fundamental conflict. Science is about exploration...have finite time and resources. You typically have 4-5 years to make discoveries and publish papers to ...Nature and Science, for example). This blog post is about how to get the most out of your postdoctoral training...your new model?  PFs need to be regularly thinking about manuscripts. The other items will follow. If you...this is a strong indicator that this person cares about your science and your development. Taken together...many PFs are reluctant to give talks or posters about their research. They dread giving a departmental...manuscript reviews, etc. You'll also meet future collaborators and get access to unpublished information and...

...settings are often limited to off-target sites of <4 bp. The off-target list is also generally weighted...PMID: 25409780. PubMed Central PMCID: PMC4246457. 4. Davidson CJ., et al. "Improving the limit of detection...et al. 2015). Meanwhile, Sanger sequencing is laborious, time consuming and cannot be applied to heterogenous...viable options for multiplex sequencing in many laboratories. Nevertheless, unbiased detection isn’t as sensitive...unbiased sequencing may not be worth the cost and labor when single clones are selected. Conversely, translational... Hough is a Biochemistry PhD candidate in the laboratory of Prof Steve Jackson at the Gurdon Institute...

... is needed to read your experimental output; and (4) the consequences to your experiment if there’s off-target...neurons (NeuN). Image from Griffin et al., 2019, Figure 4. Used under Creative Commons Attribution 4.0 International...starting the selection process. Factors to think about are (1) your experimental conditions and measurements...

...Scientists on Twitter here. 4)   If you enjoy writing and are ready for a larger commitment, start your own...take on collaborative roles in academia, and that means it’s important to know how to talk about science...talking about and sharing some science on the one you already have. Read my earlier post about Scientists...important for scientists to get out there and talk about their work. To tell others why that work is cool... than ever before for scientists to communicate about their work and their interest in science more generally...already make it a habit to talk – really talk – about the research you are doing in the lab with your ... the details of your science but also something about how science really works. 2)   Read science writing...

... getting abstracts and plans together for my next 4 webinars—I have them stacked close together for the... post if you debating about an academic or non-academic career Learn more about alternative science careers... written for selfish reasons: I wanted to learn about careers in science communication. When I started...Communication Internship with Addgene, I didn’t know a lot about scicomm, but had enjoyed writing a few Addgene guest...option. So if you’re interested in learning more about science communication careers, you’re in the right...careers here Resources on Addgene.org Learn more about careers at Addgene ...

...distributed – have changed so dramatically in the past 4 years. I kept busy for the whole poster session, talking...It's been about 14 years since I last attended a Keystone Meeting – far too long. Holding these meetings...CRISPR technology in just 2 years and excitement about how much more there is left to do with the characterization...downstream output. Tuesday morning's talks were also about circuitry and synthetic biology, and I found myself...plasmid sharing to those who were anxious to hear about what was coming next. I also got to show off our... updated Synbio page to people who were curious about these newest features of our website.  I want to...

... and technology for bioprocesses (1989): 272-278. 4. Ozturk, Sadettin S., and Bernhard O. Palsson. "Effect...resources on Addgene.org Learn about Addgene's viral service Learn about viral production Find viral protocols... By now you have probably heard about Addgene’s viral service but did you know that Addgene also performs... approach can be adapted by vector cores and laboratories alike as an easy and inexpensive method to improve...Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production." Biotechnology and ...

...fields not solely the specific area the writer works.  4. Practice writing everyday Overall, the most important...successful writer. When I originally talked to my PI about becoming a better writer, I was unsure if I should... a manuscript and critiquing it, were not too laborious, but helped build my understanding of how to generate...and the writing workshop. SWPI's writing workshop About a month later I received another email notifying... manuscript, scientists have a tendency to be elaborate and over explain the material. The point of writing...

... you can use to express your protein of interest. 4. Plasmids 101: Blue-White Screening Getting a lot ...eyes open for any new technologies that will bring about the next revolution in biological research. We’re...Plasmids 101: Control Plasmids Need help thinking about the types of controls you’ll need for your next ...Addgene Browse Our CRISPR Resources Learn More about Viral Vectors Browse the Repository  ...

...cytotoxicity against tumor targets. Nat Med. 2000 Apr;6(4):443-6. doi: 10.1038/74704. PMID: 10742152. Dunkelberger...phagocytosis.  Other functions We’re still learning about Fc effector functions and all that antibodies can...isotypes and Fc receptors that we don’t know much about yet. Who knows what kinds of functions they may ...

...be collected from heterozygous individuals. Only 1/4 of the descendants of the heterozygous plants, therefore... is difficult for new members of the lab or collaborators unfamiliar with these mutant plants. Using RUBY...resistant disease. (RUBY-Red: You can’t beet it!)   About the Author  Andrew Willoughby is a graduate student...

...so it might lend itself more readily to a switch. 4. Do you want more autonomy?  Before my master’s, I... Resources Resources on the Addgene blog Learn about career planning resources What Do I Do Now? Academic...

...2018. PMID: 29735997 bioRxiv preprint posted July 4, 2017 A new method to identify proteins occupying... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here. Listen...

...Ordering” to see what options are offered. Figure 4: Screenshot of the Addgene website. Let us know...variations in the spike protein, teaching us more about viral evolution and antibody binding. Figure...

...for individual cells. Each cell is labeled with 3-4 tags to increase the diversity of the CellTag library...Additional resources on the Addgene blog Learn more about induced pluripotent stems cells (iPSCS) and reprogramming...