Plasmids 101: Protein tags
Type
Blog Post
...such as Thioredoxin A (TrxA) that improve disulfide bond formation in order to help keep your protein soluble...
We narrowed to 315 results for: bon;
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Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog Post...addition, we have recently developed the oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method ... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog Post...Click here to view Addgene’s collection of empty backbones for constructing fluorescent fusions. Transcription... -
Luciferase Plasmid Collection
TypeCollection...Luciferase blog post . Empty Backbones Browse or search for popular empty backbone plasmids into which you ... Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs Other...Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α ...pMKV060 Firefly AtUbi10 BeYDV replicon on T-DNA backbone expressing firefly luc for monitoring plant transfection...pMKV057 Firefly AtUbi10 BeYDV replicon on T-DNA backbone expressing WUS2 and IPT, with firefly luc for ... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...interesting behaviors. There is diversity in the social bonds that they form including genetic mechanisms that... -
Finding nucleic acids with SHERLOCK and DETECTR
TypeBlog Post...O, Jinek M (2016) Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog Post...making it a more flexible cutter with the added bonus of low off-target effects. The SpG variant, discovered... -
Antibodies 101: Immunoprecipitation
TypeBlog Post...differing amounts of target proteins or antibody bonding strengths. . Steps of an IP: 1) Protein... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog Post...toxin-antitoxin system is MazE/MazF. The toxin MazF is a ribonuclease that cleaves specific sequences in RNA resulting... -
"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog Post.... These sequences were then cloned into an AAV backbone containing a minimal promoter to drive expression... -
Plasmids 101: Shuttle Vectors
TypeBlog Post...Resources on Addgene.org Collection of empty plasmid backbones ... -
Viral Production at Addgene
TypeBlog Post...responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog Post...with a protocol for a library of similar size and backbone. Verification Once your library has been amplified... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...PMC41617. 7. Metzger, D, J Clifford, H Chiba, and P Chambon. 1995. “Conditional Site-Specific Recombination... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog Post...BioRender.com. Once DnaA breaks the local hydrogen bonds holding the DNA together, it recruits helicases ... -
Viral Vectors 101: Pseudotyping
TypeBlog Post... type, but other envelopes such as the one from gibbon ape leukemia virus (GALV) are more stable (Sandrin... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog Post...oligos are required and the BsaI linearized pEcgRNA backbone can be prepared in large amounts and frozen for... -
Using Video to Share Your Science: We Share Science
TypeBlog Post...rather than on the day-to-day research that is the backbone of science. Facebook is continuing its growth,... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post...for validating your selected gRNA’s, and has the bonus benefit of supplying you with primers for validating... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post...means that the promoter present in your plasmid backbone must to be compatible with the type of RNA that...