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We narrowed to 329 results for: cloning

Showing: 201 - 220 of 329 results

  2. Open Enzyme Collection

    Type
    Collection
    ...Jennifer Molloy Lab Plasmids Blog: Golden Gate Cloning (Link opens in a new window) The Open Enzyme Collection...collection include polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription...the basis of manufacturing their own enzymes by cloning them into an expression vector of their choice ...not functional by themselves. Researchers should clone these enzymes into an expression vector of their...

  3. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...The original green fluorescent protein (GFP) was cloned in 1992 ( Prasher et al., Gene, 1992 ), and since...506 517 93 5.7 10 min Monomer pCS2+mNeonGreen-C Cloning Vector - Mammalian Expression Jump to Top Yellow...529 59 5.7 Monomer (A206K) pcDNA3 mCitrine LIC cloning vector (6C) - Mammalian Expression pCS2+8CmCitrine.../Xenopus/C.elegans/Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression mCitrine-N1... 548 562 49 6.5 2.5 hr Monomer pET mOrange LIC cloning vector (u-mOrange) - Bacterial Expression pCS2+...pmScarlet_C1 - Mammalian Expression pCS2+mScarlet-C Cloning Vector - Mammalian Expression mScarlet-I 569 593...587 610 16 4.5 15 min Monomer pcDNA3 mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry ...

  4. Plan Your Experiment

    Type
    Collection
    ...using standard restriction-ligation cloning. However, the exact cloning strategy will depend on the gRNA ...including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating...Introduction Select Genetic Manipulation Design and Clone gRNA Select Expression and Delivery Validate Genetic...the multi-plasmid SAM system Activate Design and Clone Your gRNA Achieving your desired edit comes down...few factors to keep in mind when designing and cloning your gRNAs. Select Genetic Element to be Targeted... about how to design your gRNA . Synthesize and Clone Desired gRNAs Once your target sequences are selected...selected, it’s time to design your gRNA oligos and clone them into your desired vector. In many cases, you...

  5. Gibson Assembly Protocol

    Type
    Protocol
    ... Protocols Gibson Cloning Gibson Assembly Cloning You may also like... Cloning Methods...Assembly Cloning! Protocols...biologists now use this method extensively. Why Gibson Cloning? No need for specific restriction sites. Join almost...

  7. CRISPR Plasmids - Xenopus

    Type
    Collection
    ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9...

  8. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9...

  9. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...my lab we do a lot of genetic construction and cloning experiments. Not all of our lab enzymes are made...

  14. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...promoter. WPRE has been removed to increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express...well as the Cre and Flp recombinases. Genes can be cloned into these transfer vectors using shuttle vectors...Addgene #73345) is uniquely suited to simplify the cloning process as it can be used in blue–white screening...

  15. Resolute Plasmid Collection

    Type
    Collection
    .... These vectors allow a fast and efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors...NeuroMab Plasmid Collection Addgene Blog: Gateway Cloning Content last reviewed: 12 August 2025...

  17. Hot Plasmids - February 2022

    Type
    Blog Post
    ...popularity of FLAG and HA tags, you may not even need to clone new constructs to label your favorite protein! While...
Showing: 201 - 220 of 329 results