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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 326 results for: cloning

Showing: 201 - 220 of 326 results

  2. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... for Cloning 67720 Worm NotI/BamHI none S. pyogenes Colaiacovo pRB17 52527 Fly blunt end cloning none ...or nick. Selection , such as Puromycin or EGFP Cloning enzyme used for insertion of your gRNA sequence... by the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection ...BbsI yes, cut S. pyogenes Puro Zhang sgRNA(MS2) cloning backbone 61424 Mammalian BbsI none S. pyogenes ... U6 yes, cut (enhanced) S. pyogenes Zhang gRNA Cloning Vector Bbs I ver. 2 85586 Mammalian BbsI None S...nickase expression cassette using the Golden Gate cloning method. A separate accessory pack is available ...expressed from independent promoters. sgRNAs are cloned into separate vectors and then assembled into one...

  3. Open Enzyme Collection

    Type
    Collection
    ...collection include polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription...the basis of manufacturing their own enzymes by cloning them into an expression vector of their choice ...not functional by themselves. Researchers should clone these enzymes into an expression vector of their...Plasmids Jennifer Molloy Lab Plasmids Golden Gate cloning...

  4. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...The original green fluorescent protein (GFP) was cloned in 1992 ( Prasher et al., Gene, 1992 ), and since...506 517 93 5.7 10 min Monomer pCS2+mNeonGreen-C Cloning Vector - Mammalian Expression Jump to Top Yellow...529 59 5.7 Monomer (A206K) pcDNA3 mCitrine LIC cloning vector (6C) - Mammalian Expression pCS2+8CmCitrine.../Xenopus/C.elegans/Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression mCitrine-N1... 548 562 49 6.5 2.5 hr Monomer pET mOrange LIC cloning vector (u-mOrange) - Bacterial Expression pCS2+...pmScarlet_C1 - Mammalian Expression pCS2+mScarlet-C Cloning Vector - Mammalian Expression mScarlet-I 569 593...587 610 16 4.5 15 min Monomer pcDNA3 mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry ...

  5. Gibson Assembly Protocol

    Type
    Protocol
    ... Protocols Gibson Cloning Gibson Assembly Cloning You may also like... Cloning Methods...Assembly Cloning! Protocols...biologists now use this method extensively. Why Gibson Cloning? No need for specific restriction sites. Join almost...

  10. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...my lab we do a lot of genetic construction and cloning experiments. Not all of our lab enzymes are made...

  12. CRISPR Plasmids - Xenopus

    Type
    Collection
    ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9...

  13. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ... based on factors such as selectable marker or cloning method. When using CRISPR, you will need to express...-containing plasmids. ID gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9...

  14. Plan Your Experiment

    Type
    Collection
    ...using standard restriction-ligation cloning. However, the exact cloning strategy will depend on the gRNA ...targeted region from DNA, subcloning into a plasmid, and screening individual clones. PCR amplification and...your gRNA . Browse Plasmids: gRNAs Synthesize and clone desired gRNAs Once you have selected your target...sequences it is time to design your gRNA oligos and clone these oligos into your desired vector. In many cases...whether the edit was successful. PCR amplification, subcloning and Sanger sequencing (for HDR or NHEJ): Provides...

  15. Resolute Plasmid Collection

    Type
    Collection
    ...Plasmid Collection You may also like... Gateway Cloning Brain Initiative Collection NeuroMab Collection.... These vectors allow a fast and efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors...

  19. Hot Plasmids - February 2022

    Type
    Blog Post
    ...popularity of FLAG and HA tags, you may not even need to clone new constructs to label your favorite protein! While...
Showing: 201 - 220 of 326 results